CAJ | 학술논문

目的观察芝麻素对氯化镉染毒大鼠心肌损伤的保护作用.方法 50只雄性Wistar大鼠随机分为对照组(生理盐水)、氯化镉组(腹腔注射氯化镉1.5 mg/kg·2d)、低、中、高芝麻素组(腹腔注射氯化镉1.5 mg/kg·2d后分别加20、40、80 mg/kg芝麻素),不同处理连续4周.生理记录仪测定心电图;生化法测定血清中磷酸肌酸激酶(CK)及其同工酶(CK-MB)的含量;心脏称重,部分心脏组织用于组织病理学检查,部分匀浆测定心肌组织丙二醛(MDA)含量.结果与对照组比较,氯化镉组大鼠心脏系数升高、血清中CK及CK-MB均明显升高,ST段明显抬高,心肌组织MDA含量升高,差异均有统计学意义(P＜0.05).组织病理学观察显示,氯化镉组心肌组织、细胞水肿,心肌纤维紊乱,细胞核固缩,核染色质不均匀、深染.各芝麻素剂量组可不同程度缓解氯化镉所致心肌病理学改变,以大剂量组的影响最明显.高剂量芝麻素组大鼠心脏系数(0.19％±0.01％)较氯化镉组(0.21％±0.01％)明显降低,差异有统计学意义(P＜0.05).与氯化镉组比较,中、高芝麻素剂量组血清中CK及CK-MB活力均明显降低,差异有统计学意义(P＜0.05).3个芝麻素剂量组大鼠心肌组织MDA含量[(42.32±4.65)、(36.71±5.34)、(33.12±4.62) nmol/mg pro]明显低于氯化镉组[(55.87±3.65) nmol/mg pro],差异均有统计学意义(P＜0.05).结论芝麻素可对抗氯化镉的心肌损伤,其机制可能与其增强心肌组织的抗氧化能力有关.
목적 관찰지마소대록화력염독대서심기손상적보호작용.방법 50지웅성Wistar대서수궤분위대조조(생리염수)、록화력조(복강주사록화력1.5 mg/kg·2d)、저、중、고지마소조(복강주사록화력1.5 mg/kg·2d후분별가20、40、80 mg/kg지마소),불동처리련속4주.생리기록의측정심전도;생화법측정혈청중린산기산격매(CK)급기동공매(CK-MB)적함량;심장칭중,부분심장조직용우조직병이학검사,부분균장측정심기조직병이철(MDA)함량.결과 여대조조비교,록화력조대서심장계수승고、혈청중CK급CK-MB균명현승고,ST단명현태고,심기조직MDA함량승고,차이균유통계학의의(P＜0.05).조직병이학관찰현시,록화력조심기조직、세포수종,심기섬유문란,세포핵고축,핵염색질불균균、심염.각지마소제량조가불동정도완해록화력소치심기병이학개변,이대제량조적영향최명현.고제량지마소조대서심장계수(0.19％±0.01％)교록화력조(0.21％±0.01％)명현강저,차이유통계학의의(P＜0.05).여록화력조비교,중、고지마소제량조혈청중CK급CK-MB활력균명현강저,차이유통계학의의(P＜0.05).3개지마소제량조대서심기조직MDA함량[(42.32±4.65)、(36.71±5.34)、(33.12±4.62) nmol/mg pro]명현저우록화력조[(55.87±3.65) nmol/mg pro],차이균유통계학의의(P＜0.05).결론 지마소가대항록화력적심기손상,기궤제가능여기증강심기조직적항양화능력유관.
Objective To investigate the protective effect of sesamin against cadmium chloride (CdC12)-induced cardiotoxicity in rats.Methods Fifty male Wistar rats were randomly assigned to five groups:control group,CdC12 group,and low-,middle-,and high-dose sesamin groups.The control group was given normal saline.The CdC12 group and sesamin groups were intraperitoneally injected with CdCl2 (5 mg/kg·2 d),and the low-,middle-,and high-dose sesamin groups were given 20,40,and 80 mg/kg sesamin,respectively.All treatments lasted for four weeks.ECG was measured by a physiological recorder,and serum myocardial enzyme levels were determined by biochemical assay.The heart was weighed,and heart tissues were used in histopathological examination and determination of malondialdehyde (MDA) level.Results Compared with the control group,the CdC12 group showed significantly higher levels of serum CK and CK-MB,an increased heart coefficient,significant ST-segment elevation,and higher level of MDA in myocardial tissue (P ＜0.05).Histopathological analysis showed edema of myocardial tissues and cells,myocardial fibers disorder,karyopyknosis,and uneven or deep staining of nuclear chromatin.Different doses of sesamin relieved the myocardial pathological changes induced by CdCl2,and high-dose sesamin was the most effective.The middleand high-dose sesamin groups showed significantly reduced serum CK and CK-MB levels compared with the CdC12 group (P＜0.05).The heart coefficient of the high-dose sesamin group (0.19±0.01％) was significantly lower than that of the CdCl2 group (0.21 ±0.01％) (P＜0.05).Myocardial M DA levels of the three sesamin groups (42.32±4.65,36.71 ±5.34,and 33.12±4.62 nmol/mg pro,respectively) were all significantly lower than that of the CdC12 group (55.87±3.65 nmol/mg pro) (P＜0.05).Conclusion Sesamin can relieve myocardial injury induced by CdCl2,and one possible mechanism is the enhancement of antioxidant capacity of myocardial tissue.