中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2014年
7期
487-492
,共6页
吕震%王取南%陆林玲%夏新%张龙
呂震%王取南%陸林玲%夏新%張龍
려진%왕취남%륙림령%하신%장룡
氰戊菊酯%海马%雌激素
氰戊菊酯%海馬%雌激素
청무국지%해마%자격소
Fenvalerate%Hippocampal%Estrogen
目的 探讨雌激素干扰特性在氰戊菊酯神经发育毒性中的作用.方法 4周龄健康雌性ICR小鼠30只,随机分为6组:假手术组、假手术给予氰戊菊酯(5μg/g)处理组、卵巢切除后对照组、卵巢切除后给予雌激素(E2,10μg/g)处理组、卵巢切除后给予氰戊菊酯处理组、卵巢切除后给予雌激素和氰戊菊酯处理组,每组5只,腹腔注射给药,每天1次,连续7d,末次染毒24 h后分离海马.用免疫荧光组织化学技术检测海马CA1、CA3和DG区神经元标志物(NeuN)和星形胶质细胞标志物(GFAP).结果 与假手术组[CA1(54.00±1.73)、CA3(59.00±1.73)、DG(100.00±4.58)]比较,假手术给予氰戊菊酯处理组[CA1(37.67±2.08)、CA3(41.33±1.15)、DG(80.67±0.58)]和卵巢切除后对照组[CA1(44.00±3.00)、CA3(51.00±3.00)、DG(83.00±1.72)]海马各区神经元(NeuN阳性)细胞数均明显减少,差异有统计学意义(P＜0.05).与卵巢切除后对照组比较,卵巢切除后给予氰戊菊酯处理组海马各分区NeuN阳性细胞数无明显改变.与卵巢切除后给予氰戊菊酯处理组[CA1 (47.67±3.21)、DG(87.33±4.04)]比较,假手术后给予氰戊菊酯处理和卵巢切除后给予雌激素和氰戊菊酯处理[CA1 (40.00±1.00)、DG(78.67±2.31)]NeuN阳性细胞数均明显减少,差异有统计学意义(P＜0.05).与假手术组[CA3(11.00±1.12)、DG(10.67±1.15)]比较,假手术后给予氰戊菊酯处理组[CA3(18.67±2.07)、DG(16.33±1.53)]星形胶质细胞(GFAP阳性)细胞明显增加,差异有统计学意义(P＜0.05).与假手术后给予氰戊菊酯处理组比较,卵巢切除后给予氰戊菊酯处理组[CA3(12.00±1.00)、DG(11.68±1.16)]GFAP阳性细胞明显减少,差异有统计学意义(P＜0.05).与卵巢切除后给予氰戊菊酯处理组比较,假手术后给予氰戊菊酯处理组和卵巢切除后给予雌激素和氰戊菊酯处理组[CA3(16.67±2.13)、DG(15.38±1.42)] GFAP阳性细胞数明显增加,差异有统计学意义(P＜0.05).结论 氰戊菊酯干扰循环雌激素作用是其发挥神经发育毒性的重要作用机制.
目的 探討雌激素榦擾特性在氰戊菊酯神經髮育毒性中的作用.方法 4週齡健康雌性ICR小鼠30隻,隨機分為6組:假手術組、假手術給予氰戊菊酯(5μg/g)處理組、卵巢切除後對照組、卵巢切除後給予雌激素(E2,10μg/g)處理組、卵巢切除後給予氰戊菊酯處理組、卵巢切除後給予雌激素和氰戊菊酯處理組,每組5隻,腹腔註射給藥,每天1次,連續7d,末次染毒24 h後分離海馬.用免疫熒光組織化學技術檢測海馬CA1、CA3和DG區神經元標誌物(NeuN)和星形膠質細胞標誌物(GFAP).結果 與假手術組[CA1(54.00±1.73)、CA3(59.00±1.73)、DG(100.00±4.58)]比較,假手術給予氰戊菊酯處理組[CA1(37.67±2.08)、CA3(41.33±1.15)、DG(80.67±0.58)]和卵巢切除後對照組[CA1(44.00±3.00)、CA3(51.00±3.00)、DG(83.00±1.72)]海馬各區神經元(NeuN暘性)細胞數均明顯減少,差異有統計學意義(P＜0.05).與卵巢切除後對照組比較,卵巢切除後給予氰戊菊酯處理組海馬各分區NeuN暘性細胞數無明顯改變.與卵巢切除後給予氰戊菊酯處理組[CA1 (47.67±3.21)、DG(87.33±4.04)]比較,假手術後給予氰戊菊酯處理和卵巢切除後給予雌激素和氰戊菊酯處理[CA1 (40.00±1.00)、DG(78.67±2.31)]NeuN暘性細胞數均明顯減少,差異有統計學意義(P＜0.05).與假手術組[CA3(11.00±1.12)、DG(10.67±1.15)]比較,假手術後給予氰戊菊酯處理組[CA3(18.67±2.07)、DG(16.33±1.53)]星形膠質細胞(GFAP暘性)細胞明顯增加,差異有統計學意義(P＜0.05).與假手術後給予氰戊菊酯處理組比較,卵巢切除後給予氰戊菊酯處理組[CA3(12.00±1.00)、DG(11.68±1.16)]GFAP暘性細胞明顯減少,差異有統計學意義(P＜0.05).與卵巢切除後給予氰戊菊酯處理組比較,假手術後給予氰戊菊酯處理組和卵巢切除後給予雌激素和氰戊菊酯處理組[CA3(16.67±2.13)、DG(15.38±1.42)] GFAP暘性細胞數明顯增加,差異有統計學意義(P＜0.05).結論 氰戊菊酯榦擾循環雌激素作用是其髮揮神經髮育毒性的重要作用機製.
목적 탐토자격소간우특성재청무국지신경발육독성중적작용.방법 4주령건강자성ICR소서30지,수궤분위6조:가수술조、가수술급여청무국지(5μg/g)처리조、란소절제후대조조、란소절제후급여자격소(E2,10μg/g)처리조、란소절제후급여청무국지처리조、란소절제후급여자격소화청무국지처리조,매조5지,복강주사급약,매천1차,련속7d,말차염독24 h후분리해마.용면역형광조직화학기술검측해마CA1、CA3화DG구신경원표지물(NeuN)화성형효질세포표지물(GFAP).결과 여가수술조[CA1(54.00±1.73)、CA3(59.00±1.73)、DG(100.00±4.58)]비교,가수술급여청무국지처리조[CA1(37.67±2.08)、CA3(41.33±1.15)、DG(80.67±0.58)]화란소절제후대조조[CA1(44.00±3.00)、CA3(51.00±3.00)、DG(83.00±1.72)]해마각구신경원(NeuN양성)세포수균명현감소,차이유통계학의의(P＜0.05).여란소절제후대조조비교,란소절제후급여청무국지처리조해마각분구NeuN양성세포수무명현개변.여란소절제후급여청무국지처리조[CA1 (47.67±3.21)、DG(87.33±4.04)]비교,가수술후급여청무국지처리화란소절제후급여자격소화청무국지처리[CA1 (40.00±1.00)、DG(78.67±2.31)]NeuN양성세포수균명현감소,차이유통계학의의(P＜0.05).여가수술조[CA3(11.00±1.12)、DG(10.67±1.15)]비교,가수술후급여청무국지처리조[CA3(18.67±2.07)、DG(16.33±1.53)]성형효질세포(GFAP양성)세포명현증가,차이유통계학의의(P＜0.05).여가수술후급여청무국지처리조비교,란소절제후급여청무국지처리조[CA3(12.00±1.00)、DG(11.68±1.16)]GFAP양성세포명현감소,차이유통계학의의(P＜0.05).여란소절제후급여청무국지처리조비교,가수술후급여청무국지처리조화란소절제후급여자격소화청무국지처리조[CA3(16.67±2.13)、DG(15.38±1.42)] GFAP양성세포수명현증가,차이유통계학의의(P＜0.05).결론 청무국지간우순배자격소작용시기발휘신경발육독성적중요작용궤제.
Objective To investigate the estrogen interference property of fenvalerate in ncurodevelopmental toxicity.Methods Thirty 4-week-old healthy female ICR mice were randomly divided into 6 groups:sham operation group,ovariectomized control group,ovariectomized with estrogen (10 μg/g) group,ovariectomized with fenvalerate (5 μg/g) group,sham operation with fenvalerate group,and ovariectomized with estrogen and fenvalerate group,with 5 mice in each group.Fenvalerate was injected intraperitoneally once a day for 7 consecutive days.Mice were sacrificed at 24 h after the last exposure to separate the hippocampus.Immunofluorescence was used to detect neuron marker (NeuN) and astrocyte marker (GFAP) in hippocampal CA1,CA3,and DG regions.Results Compared with the sham operation group (numbers of NeuN-positive cells:CA 1 (54.00± 1.73),CA3 (59.00± 1.73),DG (100.00±4.58)),the sham operation with fenvalerate group (CA1 (37.67±2.08),CA3 (41.33±1.15),DG (80.67±0.58)) and ovariectomized control group (CA1 (44.00± 3.00),CA3 (51.00±3.00),DG (83.00±1.72)) showed significant decreases in number of neurons (NeuNpositive cells) in the hippocampus (P＜0.05).Compared with the ovariectomized control group,the ovariectomized with fenvalerate group (CA1 (47.67±3.21),CA3 (49.00±1.73),DG (87.33±4.04)) showed no significant change in number of hippocampal NeuN-positive cells.Compared with the ovariectomized with fenvalerate group (CA1 (47.67±3.21),DG (87.33±4.04)),the sham operation with fenvalerate group and ovariectomized with estrogen and fenvalerate group (CA1 (40.00±1.00),DG (78.67±2.31)) experienced significant decreases in NeuN-positive cells (P＜0.05).Compared with the sham operation group (CA3 (11.00± 1.12),DG (10.67±1.15)),the sham operation with fenvalerate group (CA3 (18.67±2.07),DG (16.33±1.53)) showed significant increase in number of astrocytes (GFAP-positive) cells (P＜0.05).Compared with the sham operation with fenvalerate group,the ovariectomized with fenvalerate group (CA3 (12.00± 1.00),DG (11.68± 1.16)) showed significant decrease in GFAP-positive cells (P＜0.05).Compared with the ovariectomized with fenvalerate group,the sham operation with fenvalerate group and ovariectomized with estrogen and fenvalerate group (CA3 (16.67±2.13),DG (15.38±1.42)) showed significant increases in GFAP-positive cells (P＜0.05).Conclusion The interference with circulating estrogen is an important mechanism underlying the neurodevelopmental toxicity of fenvalerate.
