中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2014年
8期
573-577
,共5页
寇杰%范雪云%时亚欣%王晓艳%沈福海%金玉兰
寇傑%範雪雲%時亞訢%王曉豔%瀋福海%金玉蘭
구걸%범설운%시아흔%왕효염%침복해%금옥란
矽肺%基质金属蛋白酶%多态性,单核苷酸
矽肺%基質金屬蛋白酶%多態性,單覈苷痠
석폐%기질금속단백매%다태성,단핵감산
Silicosis%Metal matrix proteinase%Polymorphisms,single nucleotide
目的 探讨基质金属蛋白酶(MMP)-2(-735)、MMP-3(-1171)位点基因多态性与矽肺易感性的关系.方法 用病例对照研究方法,选择已确诊的113例Ⅰ期矽肺患者为病例组,以同一性别、民族、接触相同性质粉尘,年龄、累积接尘工龄相近的非矽肺接尘工人115例作为对照.应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)法检测MMP-2和-3 2个基因位点的基因型并进行分析.结果 两组研究对象的年龄、累积接尘工龄、吸烟率比较,差异均无统计学意义(P＞0.05).病例组MMP-2C-735T位点的C/C、C/T和T/T基因型分布频率分别为57.5％(65/113)、31.0％(35/113)、11.5％(13/113),与对照组[69.6％(80/115)、26.9％(31/115)和3.5％(4/115)]比较,差异有统计学意义(x2=6.542,P＜0.05);病例组和对照组T等位基因分布频率分别为27.0％和17.0％,差异有统计学意义(x2=6.704,P＜0.05),携带T等位基因患矽肺病的风险增加1.811倍(OR=1.811,95％CI:1.151～2.847);病例组MMP-3A-1171A位点的6A/6A、5A/6A和5A/5A基因型分布频率67.2％(76/113)、24.8％(28/113)、8.0％(9/113),与对照组(59.1％(68/115)、37.4％(43/115)和3.5％(4/115))比较,差异无统计学意义(x2=5.519,P＞0.05).结论 MMP-2C-735T位点的多态性与矽肺的发病有关联,携带T等位基因的接尘者会增加患矽肺的危险性,未发现MMP-3A-1171 A位点多态性与矽肺的发生发展有关联.
目的 探討基質金屬蛋白酶(MMP)-2(-735)、MMP-3(-1171)位點基因多態性與矽肺易感性的關繫.方法 用病例對照研究方法,選擇已確診的113例Ⅰ期矽肺患者為病例組,以同一性彆、民族、接觸相同性質粉塵,年齡、纍積接塵工齡相近的非矽肺接塵工人115例作為對照.應用聚閤酶鏈反應-限製性片段長度多態性(PCR-RFLP)法檢測MMP-2和-3 2箇基因位點的基因型併進行分析.結果 兩組研究對象的年齡、纍積接塵工齡、吸煙率比較,差異均無統計學意義(P＞0.05).病例組MMP-2C-735T位點的C/C、C/T和T/T基因型分佈頻率分彆為57.5％(65/113)、31.0％(35/113)、11.5％(13/113),與對照組[69.6％(80/115)、26.9％(31/115)和3.5％(4/115)]比較,差異有統計學意義(x2=6.542,P＜0.05);病例組和對照組T等位基因分佈頻率分彆為27.0％和17.0％,差異有統計學意義(x2=6.704,P＜0.05),攜帶T等位基因患矽肺病的風險增加1.811倍(OR=1.811,95％CI:1.151～2.847);病例組MMP-3A-1171A位點的6A/6A、5A/6A和5A/5A基因型分佈頻率67.2％(76/113)、24.8％(28/113)、8.0％(9/113),與對照組(59.1％(68/115)、37.4％(43/115)和3.5％(4/115))比較,差異無統計學意義(x2=5.519,P＞0.05).結論 MMP-2C-735T位點的多態性與矽肺的髮病有關聯,攜帶T等位基因的接塵者會增加患矽肺的危險性,未髮現MMP-3A-1171 A位點多態性與矽肺的髮生髮展有關聯.
목적 탐토기질금속단백매(MMP)-2(-735)、MMP-3(-1171)위점기인다태성여석폐역감성적관계.방법 용병례대조연구방법,선택이학진적113례Ⅰ기석폐환자위병례조,이동일성별、민족、접촉상동성질분진,년령、루적접진공령상근적비석폐접진공인115례작위대조.응용취합매련반응-한제성편단장도다태성(PCR-RFLP)법검측MMP-2화-3 2개기인위점적기인형병진행분석.결과 량조연구대상적년령、루적접진공령、흡연솔비교,차이균무통계학의의(P＞0.05).병례조MMP-2C-735T위점적C/C、C/T화T/T기인형분포빈솔분별위57.5％(65/113)、31.0％(35/113)、11.5％(13/113),여대조조[69.6％(80/115)、26.9％(31/115)화3.5％(4/115)]비교,차이유통계학의의(x2=6.542,P＜0.05);병례조화대조조T등위기인분포빈솔분별위27.0％화17.0％,차이유통계학의의(x2=6.704,P＜0.05),휴대T등위기인환석폐병적풍험증가1.811배(OR=1.811,95％CI:1.151～2.847);병례조MMP-3A-1171A위점적6A/6A、5A/6A화5A/5A기인형분포빈솔67.2％(76/113)、24.8％(28/113)、8.0％(9/113),여대조조(59.1％(68/115)、37.4％(43/115)화3.5％(4/115))비교,차이무통계학의의(x2=5.519,P＞0.05).결론 MMP-2C-735T위점적다태성여석폐적발병유관련,휴대T등위기인적접진자회증가환석폐적위험성,미발현MMP-3A-1171 A위점다태성여석폐적발생발전유관련.
Objective To investigate the relationship between the genetic polymorphisms of matrix metalloproteinase-2 (MMP-2) (-735) and matrix metalloproteinase-3 (MMP-3) (-1171) and the susceptibility to silicosis.Methods A case-control study was conducted in 113 patients diagnosed with stage Ⅰ silicosis (case group) and 115 dust-exposed workers without silicosis (control group); the two groups had the same sex,ethnic group,and type of dust and similar age and cumulative exposure time.Polymerase chain reaction-restriction fragment length polymorphism was used to determine the genotypes of MMP-2 (-735) and MMP-3 (-1171).Results No significant difference was observed in age,cumulative exposure time,or smoking rate between cases and controls (P＞0.05).The frequencies of genotypes C/C,C/T,and T/T at MMP-2 C-735T in the case group were 57.5％ (65/113),31.0％ (35/113),and 11.5％ (13/113),respectively,which were significantly different from those of the control group (69.6％ (80/115),26.9％ (31/115),and 3.5％ (4/115)),(x2=6.542,P＜0.05).The frequencies of T allele in cases and controls were 27.0％ and 17.0％,respectively,which were significantly different from each other (x2=6.704,P＜0.05).Carriage of T allele at MMP-2 C-735T increased the risk of silicosis (OR=1.811,95％CI:1.151-2.847).The frequencies of genotypes 6A/6A,5A/6A,and 5A/5A at MMP-3 A-1171A were 67.2％ (76/113),24.8％ (28/113),and 8.0％ (9/113),respectively,in the case group,versus 59.1％ (68/115),37.4％ (43/115),and 3.5％ (4/115) in the control group (x2=5.519,P＞0.05).Conclusion Genetic polymorphism at MMP-2 C-735T is significantly associated with the development of silicosis.Carriage of T allele increases the risk of silicosis among workers exposed to dust.No significant association was found between MMP-3 A-1171A polymorphism and silicosis in this study.
