中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2014年
8期
607-609
,共3页
王翠翠%刘晓红%毛蕊琪%曹永成%毕利泉%耿明
王翠翠%劉曉紅%毛蕊琪%曹永成%畢利泉%耿明
왕취취%류효홍%모예기%조영성%필리천%경명
减压病%肿瘤坏死因子%胶质原纤维酸性蛋白%脊髓损伤%星形胶质细胞
減壓病%腫瘤壞死因子%膠質原纖維痠性蛋白%脊髓損傷%星形膠質細胞
감압병%종류배사인자%효질원섬유산성단백%척수손상%성형효질세포
Decompression disease%Tumor necrosis factor-alpha%Glial fibrillary acidic protein%Spinal injury%Astrocyte
目的 探讨TNF-α、GFAP基因在减压病过程中的表达变化及作用机制.方法 成年健康雄性新西兰兔21只,随机分为正常组、减压病组、安全减压组,每组7只.采用快速减压方法制备减压病模型.借助光镜、荧光实时定量RT-PCR法和免疫组化方法观察脑和脊髓的病理形态学及TNF-α,GFAP基因mRNA和蛋白的表达变化.结果 减压病组脊髓白质内空泡形成,脊髓中TNF-α、GFAP基因mRNA和蛋白表达量明显增加,与正常对照组和安全减压组相比,差异有统计学意义(P＜0.01);脑内减压病组、正常对照组和安全减压组之间TNF-α、GFAP基因mRNA和蛋白的表达量未见明显差异(P＞0.05).结论 脊髓是减压病致中枢神经系统损伤的主要部位,减压病早期即可引起TNF-α和GFAP基因活化及相应蛋白大量表达,星形胶质细胞及促炎因子TNF-α在减压病脊髓损伤病变进程中发挥了重要作用.
目的 探討TNF-α、GFAP基因在減壓病過程中的錶達變化及作用機製.方法 成年健康雄性新西蘭兔21隻,隨機分為正常組、減壓病組、安全減壓組,每組7隻.採用快速減壓方法製備減壓病模型.藉助光鏡、熒光實時定量RT-PCR法和免疫組化方法觀察腦和脊髓的病理形態學及TNF-α,GFAP基因mRNA和蛋白的錶達變化.結果 減壓病組脊髓白質內空泡形成,脊髓中TNF-α、GFAP基因mRNA和蛋白錶達量明顯增加,與正常對照組和安全減壓組相比,差異有統計學意義(P＜0.01);腦內減壓病組、正常對照組和安全減壓組之間TNF-α、GFAP基因mRNA和蛋白的錶達量未見明顯差異(P＞0.05).結論 脊髓是減壓病緻中樞神經繫統損傷的主要部位,減壓病早期即可引起TNF-α和GFAP基因活化及相應蛋白大量錶達,星形膠質細胞及促炎因子TNF-α在減壓病脊髓損傷病變進程中髮揮瞭重要作用.
목적 탐토TNF-α、GFAP기인재감압병과정중적표체변화급작용궤제.방법 성년건강웅성신서란토21지,수궤분위정상조、감압병조、안전감압조,매조7지.채용쾌속감압방법제비감압병모형.차조광경、형광실시정량RT-PCR법화면역조화방법관찰뇌화척수적병리형태학급TNF-α,GFAP기인mRNA화단백적표체변화.결과 감압병조척수백질내공포형성,척수중TNF-α、GFAP기인mRNA화단백표체량명현증가,여정상대조조화안전감압조상비,차이유통계학의의(P＜0.01);뇌내감압병조、정상대조조화안전감압조지간TNF-α、GFAP기인mRNA화단백적표체량미견명현차이(P＞0.05).결론 척수시감압병치중추신경계통손상적주요부위,감압병조기즉가인기TNF-α화GFAP기인활화급상응단백대량표체,성형효질세포급촉염인자TNF-α재감압병척수손상병변진정중발휘료중요작용.
Objective To investigate the changes in expression of tumor necrosis factor-alpha (TNF-α) and glial fibrillary acidic protein (GFAP) in rabbits with decompression disease (DCS),and to investigate the functioning mechanism.Methods A total of 21 healthy adult rabbits were randomly divided into 3 groups:normal control group,DCS group,and safe relief group,with 7 rabbits in each group.A rabbit DCS model was established by quick decompression.The changes in pathological morphology and mRNA and protein expression of TNF-α and GFAP in the brain and spinal cord of rabbits with DCS were determined by light microscopy,real-time PCR,and immunohistochemistry,respectively.Results Cavity formation was observed in the white matter of spinal cord in DCS group.The mRNA and protein expression of TNF-α and GFAP was significantly higher in the DCS group than in the normal control group and safe relief group (P＜0.01),while no significant differences were observed in the brain (P＞0.05).Conclusion Spinal cord is the main part of central nervous system injury in DCS.Activation of TNF-α and GFAP genes accompanied by increase in their protein expression can be observed at the early stage of DCS.The astrocytes and TNF-α play important roles in the process of spinal cord injury in DCS.
