CAJ | 학술논문

目的探讨河南汉族老年患者纤溶酶原激活抑制物(PAI-1)基因多态性与缺血性脑血管病的关系及其与相关的危险因素的相互作用. 方法应用病例对照研究方法,选取408例缺血性脑血管病患者以及年龄、性别匹配的418例健康对照人群为研究对象,采用聚合酶链式反应-限制性片段多态性(PCR-RFLP)法进行PAI-1-844G/A多态性位点检测. 结果患者组PAI-1-844G/A位点AA、AG、GG基因型频率依次为18.1％、41.4％、40.4％,对照组分别为15.8％、47.4％、36.8％；患者组和对照组A等位基因频率分别为38.8％、39.5％,G等位基因频率分别为61.2％、60.5％.PAI-1-844G/A位点基因型和等位基因频率差异均无统计学意义(P＞0.05).PAI-1基因844G/A位点多态性的不同基因型与缺血性脑血管病患者的血压、血糖、血浆同型半胱氨酸(Hcy)水平等指标间无相关性(均P＞0.05). 结论 PAI-1基因-844G/A位点多态性可能不是河南汉族老年人群缺血性脑血管病发病的独立危险因素.
목적 탐토하남한족노년환자섬용매원격활억제물(PAI-1)기인다태성여결혈성뇌혈관병적관계급기여상관적위험인소적상호작용. 방법 응용병례대조연구방법,선취408례결혈성뇌혈관병환자이급년령、성별필배적418례건강대조인군위연구대상,채용취합매련식반응-한제성편단다태성(PCR-RFLP)법진행PAI-1-844G/A다태성위점검측. 결과 환자조PAI-1-844G/A위점AA、AG、GG기인형빈솔의차위18.1％、41.4％、40.4％,대조조분별위15.8％、47.4％、36.8％；환자조화대조조A등위기인빈솔분별위38.8％、39.5％,G등위기인빈솔분별위61.2％、60.5％.PAI-1-844G/A위점기인형화등위기인빈솔차이균무통계학의의(P＞0.05).PAI-1기인844G/A위점다태성적불동기인형여결혈성뇌혈관병환자적혈압、혈당、혈장동형반광안산(Hcy)수평등지표간무상관성(균P＞0.05). 결론 PAI-1기인-844G/A위점다태성가능불시하남한족노년인군결혈성뇌혈관병발병적독립위험인소.
Objective To investigate the association between gene polymorphism of plasminogen activator inhibitor (PAI-1)and ischemic cerebrovascular disease in aged people of Henan Han nationality.Methods A case control method was used,including 408 patients with ischemic cerebrovascular disease and 418 age and gender matched healthy controls.Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) method was used to determine the distribution of allele and genotype frequencies of PAI-1 844G/A.Results In patient group,the gene frequencies of AA,AG,GG were 18.1％,41.4％,40.4％,and the A and G allele frequencies were 38.8％ and 61.2％,respectively.In the control group,the gene frequencies of AA,AG,GG were 15.8％,47.4％,36.8％,and the A and G allele frequencies were 39.5％ and 60.5％.There were no significant differences in the frequencies of genotypes and alleles between the two groups (P＞0.05).The different genotypes of 844G/A of PAI-1 gene were not associated with hypertension,hyperglycemia,plasma homocysteic acid levels (P ＞ 0.05).Conclusions PAI-1 gene-844G/A polymorphism may not be independent risk factor of ischemic cerebrovascular disease in aged people of Henan Han nationality.