CAJ | 학술논문

目的探讨缺氧诱导因子-1α(HIF-1α)在缺氧后大脑皮层神经元的表达情况,为临床治疗缺血缺氧性脑血管病提供更多的实验依据.方法制备体外培养大鼠大脑皮层神经元原代培养及缺氧的模型;免疫细胞化学鉴定大鼠大脑皮层神经元;在12h,24 h,48 h及72 h采用免疫组化方法检测正常及缺氧神经元中HIF-1α的表达情况.结果正常对照组神经元各时间点HIF-1α阳性细胞表达较少,组内各时间点比较差异无统计学意义.缺氧组神经元在缺氧12 h时已观察到HIF-1α的弱阳性表达,随缺氧时间的延长阳性表达增强,24 h表达明显增加(光密度为0.27±0.02,F＝35.703,t＝11.795,P＜0.01),48 h达高峰,一直持续到72 h下降,两组各时间点相比差异有统计学意义.结论神经元缺氧后HIF-1α表达上升,对缺氧后的神经元起保护作用.
목적 탐토결양유도인자-1α(HIF-1α)재결양후대뇌피층신경원적표체정황,위림상치료결혈결양성뇌혈관병제공경다적실험의거.방법 제비체외배양대서대뇌피층신경원원대배양급결양적모형;면역세포화학감정대서대뇌피층신경원;재12h,24 h,48 h급72 h채용면역조화방법검측정상급결양신경원중HIF-1α적표체정황.결과 정상대조조신경원각시간점HIF-1α양성세포표체교소,조내각시간점비교차이무통계학의의.결양조신경원재결양12 h시이관찰도HIF-1α적약양성표체,수결양시간적연장양성표체증강,24 h표체명현증가(광밀도위0.27±0.02,F＝35.703,t＝11.795,P＜0.01),48 h체고봉,일직지속도72 h하강,량조각시간점상비차이유통계학의의.결론 신경원결양후HIF-1α표체상승,대결양후적신경원기보호작용.
Objective To investigate the expression of hypoxia-inducible factor 1α (HIF-1α)gene in rat cerebral cortex neurons under hypoxia conditions,and provide more experimental basis for clinical treatment of hypoxic brain disease.Methods The rat cerebral cortical neurons in primary culture and hypoxia model were prepared and identified by immunocytochemistry analysis.The expression of HIF-1α in normal and hypoxic neurons was detected at 4 time points (12 h,24 h,48 h and 72 h) by immunohistochemical analysis.Results The expression of HIF-1α positive cells in normal control (NC) group was less at each time point,and it showed no statistically significant within groups.The weak expression of HIF-1α was found at 12 h in hypoxic group,and the expression augmented along with the time extended,it increased obviously at 24 h,reached the peak at 48 h(IOD＝0.27±0.02,F＝35.703,t＝11.795,P＜0.01),and declined until 72 h,it showed statistical significance between two groups at each time point.Conclusions The expression of HIF-1α is increased after hypoxia.HIF-1α has a protective effect on neurons after hypoxia.