CAJ | 학술논문

目的观察根皮苷对2型糖尿病小鼠主动脉的保护作用,探讨其可能的作用机制.方法以db/m小鼠8只作为正常对照组(对照组),16只糖尿病db/db小鼠随机分为2组:糖尿病组和糖尿病干预组.干预组给根皮苷(20mg/kg)盐水灌胃10周.对照组和糖尿病组小鼠每天用相同体积的生理盐水灌胃10周.所有小鼠每周称体质量.10周末空腹处死全部小鼠,留取血清和主动脉.检测血清空腹血糖、三酰甘油、总胆固醇、糖基化终产物和丙二醛浓度.主动脉做病理学检查.结果 10周末,糖尿病组体质量(57.53±3.40)g、空腹血糖(31.21±2.16)mmol/L、三酰甘油(0.39±0.12)mmol/L、总胆固醇(3.15±0.30) mmol/L、糖基化终产物(0.28±0.04) AU/mg和丙二醛(15.18±1.60)mol/L水平高于对照组(P＜0.01).干预组体质量(54.24±1.28)g、血清空腹血糖(29.17±1.41)mmol/L、三酰甘油(0.26±0.06) mmol/L、总胆固醇(2.71±0.26) mmol/L、糖基化终产物(0.24±0.03) AU/mg和丙二醛(13.46±1.28) nmol/L水平较糖尿病组降低(P＜0.05或P＜0.01).干预组主动脉病变较糖尿病组减轻. 结论根皮苷对糖尿病小鼠的大血管具有保护作用,该作用与其降糖调脂、降低糖基化终产物水平和抗氧化有关.
목적 관찰근피감대2형당뇨병소서주동맥적보호작용,탐토기가능적작용궤제.방법 이db/m소서8지작위정상대조조(대조조),16지당뇨병db/db소서수궤분위2조:당뇨병조화당뇨병간예조.간예조급근피감(20mg/kg)염수관위10주.대조조화당뇨병조소서매천용상동체적적생리염수관위10주.소유소서매주칭체질량.10주말공복처사전부소서,류취혈청화주동맥.검측혈청공복혈당、삼선감유、총담고순、당기화종산물화병이철농도.주동맥주병이학검사.결과 10주말,당뇨병조체질량(57.53±3.40)g、공복혈당(31.21±2.16)mmol/L、삼선감유(0.39±0.12)mmol/L、총담고순(3.15±0.30) mmol/L、당기화종산물(0.28±0.04) AU/mg화병이철(15.18±1.60)mol/L수평고우대조조(P＜0.01).간예조체질량(54.24±1.28)g、혈청공복혈당(29.17±1.41)mmol/L、삼선감유(0.26±0.06) mmol/L、총담고순(2.71±0.26) mmol/L、당기화종산물(0.24±0.03) AU/mg화병이철(13.46±1.28) nmol/L수평교당뇨병조강저(P＜0.05혹P＜0.01).간예조주동맥병변교당뇨병조감경. 결론 근피감대당뇨병소서적대혈관구유보호작용,해작용여기강당조지、강저당기화종산물수평화항양화유관.
Objective To observe the effects of phlorizin on aorta of diabetes db/db mice and to explore its mechanism.Methods Sixteen diabetes db/db mice were randomized into two groups:untreated diabetic group (DM group) and diabetic group treated by phlorizin(DMT group) with eight db/m mice as normal control group(CC group).Phlorizin (20 mg/kg) was given in normal saline solution intragastrically to the mice of the DMT group for 10 weeks.Mice of the other two groups were given the same amount of saline solution intragastrically for 10 weeks.Animals were weighed weekly.At 10th weekend,all mice were fasted overnight and then sacrificed.Fasting blood was collected,and aortas were dissected.The blood samples were analyzed for fasting blood glucose (FBG),triglycerides (TG),total cholesterol (TC),Serum advanced glycation end products(AGEs)and malondialdehyde (MDA).Aortic tissue were examined microscopically.Results At 10th weekend,the weight (57.53±3.40)g and serum concentration of FBG(31.21±2.16) mmol/L,TG (0.39±0.12) mmol/L,TC(3.15±0.30)mmol/L,AGEs (0.28±0.04) AU/mg and MDA (15.18± 1.60)mol/L in DM group were increased than those in CC group (P＜0.01),and the weight(54.24± 1.28)g,FBG(29.17±1.41) mmol/L,TG(0.26±0.06) mmol/L,TC(2.71±0.26) mmol/L,AGEs (0.24±0.03) AU/mg and MDA(13.46±1.28)mol/L were lowered significantly in DMT group than those in DM group (P＜0.05 or P＜0.01).The severity of aorta damage in DMT group was less than that in DM group.Conclusions Phlorizin can protect the db/db mice from diabetic macrovascular complications,which may be attributed to its decreasing of blood glucose,TG,TC,and AGEs levels,and its antioxidant potential.