中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2013年
3期
300-304
,共5页
孙亮%郑陈光%吕泽平%胡才友%黄泽治%梁庆华%陈少科%陈科%范歆%吕渊%杨泽
孫亮%鄭陳光%呂澤平%鬍纔友%黃澤治%樑慶華%陳少科%陳科%範歆%呂淵%楊澤
손량%정진광%려택평%호재우%황택치%량경화%진소과%진과%범흠%려연%양택
长寿%过氧化物酶体增殖物激活受体%载脂蛋白E类%代谢
長壽%過氧化物酶體增殖物激活受體%載脂蛋白E類%代謝
장수%과양화물매체증식물격활수체%재지단백E류%대사
Longevity%Peroxisome proliferators activated receptors%Apolipoprotein E%Metabolism
目的 研究广西永福汉族长寿人群(年龄＞90岁)中过氧化物酶体增殖物激活受体γ辅激活因子1 (PPARGC1)基因Gly482Ser变异和载脂蛋白E(ApoE)基因变异的特征性分布规律,并评价Gly482Ser与ApoE基因的相互作用关系. 方法 基于本课题组2008-2011年在广西永福长寿之乡的现场工作,以长寿区长寿老年人212例(年龄90～105岁)为长寿组,永福无长寿家族史的非长寿人群207例为对照组.通过入户调查,收集长寿相关的体征、血糖、血脂、血压.提取外周血DNA,分型PPARGC1基因Gly482Ser变异和ApoE基因变异,建立基因组和表型相关数据库.通过单因素分析和多因素遗传统计分析,研究基因组变异和长寿及相关代谢性状的相关. 结果 与对照组比较,长寿组空腹血糖、总胆固醇和低密度脂蛋白胆固醇均低于对照组.Gly482Ser在全部人群中进行分型,Hardy-Weinberg平衡检验表明在各组中都具有较好的人群代表性,多重比较的Bonferroni法校正后,隐性遗传模型GG基因型与长寿无相关性.按ApoEε4等位基因分层分析隐性遗传模型表明,在非ApoEε4等位基因的携带者中,GG基因型与长寿呈正相关,经Bonferroni法校正后,差异有统计学意义(OR=1.72,P＜0.05).此外,长寿老人中携带GG基因型的空腹血糖低(P＜0.05),高密度脂蛋白胆固醇水平高(P＜0.05). 结论 广西永福汉族长寿老人较对照组具有更好的代谢状态,PPARGC1基因Gly482Ser变异GG基因型与长寿呈正相关,其依赖于不携带ApoE基因风险等位基因ε4而存在.
目的 研究廣西永福漢族長壽人群(年齡＞90歲)中過氧化物酶體增殖物激活受體γ輔激活因子1 (PPARGC1)基因Gly482Ser變異和載脂蛋白E(ApoE)基因變異的特徵性分佈規律,併評價Gly482Ser與ApoE基因的相互作用關繫. 方法 基于本課題組2008-2011年在廣西永福長壽之鄉的現場工作,以長壽區長壽老年人212例(年齡90～105歲)為長壽組,永福無長壽傢族史的非長壽人群207例為對照組.通過入戶調查,收集長壽相關的體徵、血糖、血脂、血壓.提取外週血DNA,分型PPARGC1基因Gly482Ser變異和ApoE基因變異,建立基因組和錶型相關數據庫.通過單因素分析和多因素遺傳統計分析,研究基因組變異和長壽及相關代謝性狀的相關. 結果 與對照組比較,長壽組空腹血糖、總膽固醇和低密度脂蛋白膽固醇均低于對照組.Gly482Ser在全部人群中進行分型,Hardy-Weinberg平衡檢驗錶明在各組中都具有較好的人群代錶性,多重比較的Bonferroni法校正後,隱性遺傳模型GG基因型與長壽無相關性.按ApoEε4等位基因分層分析隱性遺傳模型錶明,在非ApoEε4等位基因的攜帶者中,GG基因型與長壽呈正相關,經Bonferroni法校正後,差異有統計學意義(OR=1.72,P＜0.05).此外,長壽老人中攜帶GG基因型的空腹血糖低(P＜0.05),高密度脂蛋白膽固醇水平高(P＜0.05). 結論 廣西永福漢族長壽老人較對照組具有更好的代謝狀態,PPARGC1基因Gly482Ser變異GG基因型與長壽呈正相關,其依賴于不攜帶ApoE基因風險等位基因ε4而存在.
목적 연구엄서영복한족장수인군(년령＞90세)중과양화물매체증식물격활수체γ보격활인자1 (PPARGC1)기인Gly482Ser변이화재지단백E(ApoE)기인변이적특정성분포규률,병평개Gly482Ser여ApoE기인적상호작용관계. 방법 기우본과제조2008-2011년재엄서영복장수지향적현장공작,이장수구장수노년인212례(년령90～105세)위장수조,영복무장수가족사적비장수인군207례위대조조.통과입호조사,수집장수상관적체정、혈당、혈지、혈압.제취외주혈DNA,분형PPARGC1기인Gly482Ser변이화ApoE기인변이,건립기인조화표형상관수거고.통과단인소분석화다인소유전통계분석,연구기인조변이화장수급상관대사성상적상관. 결과 여대조조비교,장수조공복혈당、총담고순화저밀도지단백담고순균저우대조조.Gly482Ser재전부인군중진행분형,Hardy-Weinberg평형검험표명재각조중도구유교호적인군대표성,다중비교적Bonferroni법교정후,은성유전모형GG기인형여장수무상관성.안ApoEε4등위기인분층분석은성유전모형표명,재비ApoEε4등위기인적휴대자중,GG기인형여장수정정상관,경Bonferroni법교정후,차이유통계학의의(OR=1.72,P＜0.05).차외,장수노인중휴대GG기인형적공복혈당저(P＜0.05),고밀도지단백담고순수평고(P＜0.05). 결론 엄서영복한족장수노인교대조조구유경호적대사상태,PPARGC1기인Gly482Ser변이GG기인형여장수정정상관,기의뢰우불휴대ApoE기인풍험등위기인ε4이존재.
Objective To explore the association of peroxisome proliferators-activated receptor-γ coactivator-1 (PPARGC1) Gly482Ser with apolipoprotein E (ApoE) variations in longevity (aged above 90 yrs) Hans in Guangxi Yongfu and to explore the potential association between the variations and metabolic traits.Methods Based on our survey in Guangxi Yongfu in 2008-2011,212 elderly cases (aged 90～105 years) were included as longevity group and 207 cases without longevity history were included as control group.By household survey,we collected the longevity related parameters,blood glucose,blood lipid,blood pressure and other related metabolic traits.Peripheral blood was collected to extract DNA,the gene variations of Gly482Ser and ApoE were genotyped,and the database with genome and traits information were set up.By univariate analysis and multivariate genetic statistical analysis,the association between the variations and longevity and metabolic traits was assessed.Results Compared with the control group,the levels of fasting blood glucose,total cholesterol and low density lipoprotein were lower in the longevity group.Gly482Ser was genotyped in all samples and fully fulfilled the Hardy Weinberg equilibrium.After the Bonferroni correction,recessive model failed to find association between GG genotype and longevity.Stratified analyses by ApoEε4 allele revealed that,in the subgroup with no ApoEε4,PPARGC-1 GG genotype was positively associated with longevity in the recessive model,even after Bonferroni correction (OR =1.72,P＜0.05).In addition,longevity group with Gly482Ser GG genotype seemed to have relativelower fasting blood glucose (P ＜ 0.05) and higher high density lipoprotein levels (P ＜ 0.05).Conclusions Longevity Hans in Guangxi Yongfu preserve better metabolic state compared with the control group.GG genotype of Gly482Ser in PPARGC-1 is positively associated with longevity,which depends on not carrying the risk allele of ApoE ε4.
