中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2013年
4期
440-443
,共4页
张金%郭军红%严澎%王慧芳
張金%郭軍紅%嚴澎%王慧芳
장금%곽군홍%엄팽%왕혜방
脑缺血%红细胞生成素,重组%肿瘤坏死因子α%白细胞介素6
腦缺血%紅細胞生成素,重組%腫瘤壞死因子α%白細胞介素6
뇌결혈%홍세포생성소,중조%종류배사인자α%백세포개소6
Brain ischemia%Erythropoietin,recombinant%Tumor necrosis factor-alpha%Interleukin-6
目的 观察重组人促红细胞生成素(rhEPO)对大鼠永久性脑缺血脑组织中肿瘤坏死因子α(TNF-a)、白细胞介素6(IL-6)表达的影响,探讨其对脑缺血的保护作用及作用机制. 方法 采用血管内线栓法制备大鼠永久性局灶性脑缺血(pMCAO)模型.健康雄性SD大鼠36只,随机分为假手术组(12只),模型组(12只),手术后rhEPO治疗组(12只),手术后rhEPO治疗组在缺血2h后腹腔注射rhEPO 5000 U/kg,模型组和假手术组在缺血2h后腹腔内注射等量的生理盐水.各组随机选6只用于2,3,5-氯化三苯基四氮唑(TTC)法测量脑梗死体积,另6只用于免疫组织化学方法测定TNFα、IL-6的表达. 结果 TTC测定脑梗死体积显示,假手术组、模型组和rhEPO治疗组脑梗死体积百分比分别为0、(36.67±2.40)％和(27.49±1.47)％(F=823.50,P＜0.01),与模型组及假手术组比较,rhEPO治疗组脑梗死体积明显减小(q=7.98、45.81,均P＜0.01).免疫组织化学检测结果显示,假手术组、模型组和rhEPO治疗组脑组织TNF-α阳性细胞数分别为(9.00±1.41)个、(27.83±2.48)个、(17.50±1.87)个,IL-6阳性细胞数分别为(8.94±2.31)个、(20.33±3.53)个、(14.83±1.70)个；模型组与假手术组比较,TNF-α、IL-6表达均有所增加(q=16.1、19.6,均P＜0.01),而rhEPO治疗组较模型组TNF-a和IL-6的表达有所下降(q=8.19、3.44,均P＜0.01). 结论 在大鼠缺血2h后腹腔内注射rhEPO可以缩小大鼠脑缺血24 h后的脑梗死体积；脑缺血后TNF-α及IL-6的表达升高,rhEPO可能通过降低TNF-a及IL-6的活性来发挥其神经保护作用.
目的 觀察重組人促紅細胞生成素(rhEPO)對大鼠永久性腦缺血腦組織中腫瘤壞死因子α(TNF-a)、白細胞介素6(IL-6)錶達的影響,探討其對腦缺血的保護作用及作用機製. 方法 採用血管內線栓法製備大鼠永久性跼竈性腦缺血(pMCAO)模型.健康雄性SD大鼠36隻,隨機分為假手術組(12隻),模型組(12隻),手術後rhEPO治療組(12隻),手術後rhEPO治療組在缺血2h後腹腔註射rhEPO 5000 U/kg,模型組和假手術組在缺血2h後腹腔內註射等量的生理鹽水.各組隨機選6隻用于2,3,5-氯化三苯基四氮唑(TTC)法測量腦梗死體積,另6隻用于免疫組織化學方法測定TNFα、IL-6的錶達. 結果 TTC測定腦梗死體積顯示,假手術組、模型組和rhEPO治療組腦梗死體積百分比分彆為0、(36.67±2.40)％和(27.49±1.47)％(F=823.50,P＜0.01),與模型組及假手術組比較,rhEPO治療組腦梗死體積明顯減小(q=7.98、45.81,均P＜0.01).免疫組織化學檢測結果顯示,假手術組、模型組和rhEPO治療組腦組織TNF-α暘性細胞數分彆為(9.00±1.41)箇、(27.83±2.48)箇、(17.50±1.87)箇,IL-6暘性細胞數分彆為(8.94±2.31)箇、(20.33±3.53)箇、(14.83±1.70)箇；模型組與假手術組比較,TNF-α、IL-6錶達均有所增加(q=16.1、19.6,均P＜0.01),而rhEPO治療組較模型組TNF-a和IL-6的錶達有所下降(q=8.19、3.44,均P＜0.01). 結論 在大鼠缺血2h後腹腔內註射rhEPO可以縮小大鼠腦缺血24 h後的腦梗死體積；腦缺血後TNF-α及IL-6的錶達升高,rhEPO可能通過降低TNF-a及IL-6的活性來髮揮其神經保護作用.
목적 관찰중조인촉홍세포생성소(rhEPO)대대서영구성뇌결혈뇌조직중종류배사인자α(TNF-a)、백세포개소6(IL-6)표체적영향,탐토기대뇌결혈적보호작용급작용궤제. 방법 채용혈관내선전법제비대서영구성국조성뇌결혈(pMCAO)모형.건강웅성SD대서36지,수궤분위가수술조(12지),모형조(12지),수술후rhEPO치료조(12지),수술후rhEPO치료조재결혈2h후복강주사rhEPO 5000 U/kg,모형조화가수술조재결혈2h후복강내주사등량적생리염수.각조수궤선6지용우2,3,5-록화삼분기사담서(TTC)법측량뇌경사체적,령6지용우면역조직화학방법측정TNFα、IL-6적표체. 결과 TTC측정뇌경사체적현시,가수술조、모형조화rhEPO치료조뇌경사체적백분비분별위0、(36.67±2.40)％화(27.49±1.47)％(F=823.50,P＜0.01),여모형조급가수술조비교,rhEPO치료조뇌경사체적명현감소(q=7.98、45.81,균P＜0.01).면역조직화학검측결과현시,가수술조、모형조화rhEPO치료조뇌조직TNF-α양성세포수분별위(9.00±1.41)개、(27.83±2.48)개、(17.50±1.87)개,IL-6양성세포수분별위(8.94±2.31)개、(20.33±3.53)개、(14.83±1.70)개；모형조여가수술조비교,TNF-α、IL-6표체균유소증가(q=16.1、19.6,균P＜0.01),이rhEPO치료조교모형조TNF-a화IL-6적표체유소하강(q=8.19、3.44,균P＜0.01). 결론 재대서결혈2h후복강내주사rhEPO가이축소대서뇌결혈24 h후적뇌경사체적；뇌결혈후TNF-α급IL-6적표체승고,rhEPO가능통과강저TNF-a급IL-6적활성래발휘기신경보호작용.
Objective To investigate the effects of recombinant human erythropoietin(rhEPO)on expressions of tumon necrosis factor-alpha(TNF-α) and inter leukin-6(IL-6) in rats after focal cerebral ischemia and to explore its neuroprotective mechanism.Methods A total of 36 healthy male SD rats were randomly divided into sham-operated group (n=12),model group (n=12) and rhEPO treatment group (n=12).The suture method to make permanent middle cerebral artery occlusion model was adopted.rhEPO treatment group was injected with rhEPO 5000 U/kg intraperitoneally after 2 h of ischemia,whereas model group and sham-operated group were given identical saline at the same time.All rats were decapitated after 24 h of ischemia.6 rats were randomly selected in each group and the infarct volume of groups were measured by Triphenyl tetrazolium chloride (TTC)staining method.The expressions of TNF-α,IL-6 in other rats were detected by immunohistochemistry.Results No infarction was found in sham-operated group.Percentage of infarct volume in model group and rhEPO group were (36.672.40)％ and (27.49± 1.47)％,respectively.Compared with the model group,the volume of infarction in rhEPO group was significantly reduced.Cells stained by immunohistochemistry showed that The numbers of TNF-α-positive cells in the 3 groups were 9.001.41,27.83±2.48,17.50±1.87 and IL 6 positive cells were 8.94±2.31,20.33±3.53,14.83±1.70,respectively.Compared with sham operated group,the expressions of TNF-α and IL 6 in model group were significantly increased (q=16.1,19.6,P＜0.01).Compared with the model group,the expressions of TNF α and IL-6 in rhEPO group were significantly decreased (q=8.19,3.44,all P＜0.01).Conclusions rhEPO can decrease the infarct volume in SD rats after acute focal cerebral ischemic injure.rhEPO might exert its neuroprotective effect by reducing the expressions of TNF α and IL-6.
