中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2013年
7期
695-698
,共4页
张秀锦%李小鹰%曹甜甜%叶玲
張秀錦%李小鷹%曹甜甜%葉玲
장수금%리소응%조첨첨%협령
冠心病%受体,雄激素%多态性,单核苷酸
冠心病%受體,雄激素%多態性,單覈苷痠
관심병%수체,웅격소%다태성,단핵감산
Coronary disease%Receptor,androgen%Polymorphism,single nucleotide
目的 探讨雄激素受体基因CAG重复数多态性对老年男性冠心病发病风险的影响及可能机制. 方法 选择296例有冠状动脉造影证据的老年男性,检测血清中游离睾酮(FT)和总睾酮(TT)水平,流式细胞术检测外周血淋巴细胞雄激素受体(AR)表达水平,并提取全血基因组DNA,通过聚合酶链反应扩增AR基因片段(含CAG重复数多态性位点),所得产物经微卫星扫描分析和基因分型,最终得到相应的AR CAG重复数. 结果 老年男性患者的AR CAG重复数波动在11～28(P25～P75:18～22;中位数为20),以P75％为界分为长AR组[(CAG)n≥22,82例]和短AR组(CAG)n＜22,214例].两组比较,短AR组的FT水平低于长AR组(24.1±23.1)×10-6 mmol/L与(31.2±27.8)×10-6mmol/L (P＜0.05),短AR组合并冠心病者FT水平低于对照组(22.4±20.5)×10-6mmol/L与(33.6±32.4)×10-6 mmol/L (P＜0.01)；而短AR组冠心病发病率84.1％ (180例)高于长AR组69.5％(57例)(P＜0.01).长AR组与短AR组的TT水平和淋巴细胞AR表达水平差异无统计学意义.未发现AR基因(CAG)n重复数多态性对雄激素水平及淋巴细胞表达水平有影响,年龄是FT和AR水平的主要影响因素.Logistic回归分析结果显示,FT水平与冠心病风险呈负相关(OR=0.98,95％CI:0.973～0.998,P=0.01),短AR增加冠心病发病风险(OR=3.44,95％CI:1.887～6.264,P＜0.01). 结论 在老年男性中,雄激素受体CAG重复数短者,年龄相关的雄激素(主要是FT)水平下降更显著,与冠心病发病风险增加有关.
目的 探討雄激素受體基因CAG重複數多態性對老年男性冠心病髮病風險的影響及可能機製. 方法 選擇296例有冠狀動脈造影證據的老年男性,檢測血清中遊離睪酮(FT)和總睪酮(TT)水平,流式細胞術檢測外週血淋巴細胞雄激素受體(AR)錶達水平,併提取全血基因組DNA,通過聚閤酶鏈反應擴增AR基因片段(含CAG重複數多態性位點),所得產物經微衛星掃描分析和基因分型,最終得到相應的AR CAG重複數. 結果 老年男性患者的AR CAG重複數波動在11～28(P25～P75:18～22;中位數為20),以P75％為界分為長AR組[(CAG)n≥22,82例]和短AR組(CAG)n＜22,214例].兩組比較,短AR組的FT水平低于長AR組(24.1±23.1)×10-6 mmol/L與(31.2±27.8)×10-6mmol/L (P＜0.05),短AR組閤併冠心病者FT水平低于對照組(22.4±20.5)×10-6mmol/L與(33.6±32.4)×10-6 mmol/L (P＜0.01)；而短AR組冠心病髮病率84.1％ (180例)高于長AR組69.5％(57例)(P＜0.01).長AR組與短AR組的TT水平和淋巴細胞AR錶達水平差異無統計學意義.未髮現AR基因(CAG)n重複數多態性對雄激素水平及淋巴細胞錶達水平有影響,年齡是FT和AR水平的主要影響因素.Logistic迴歸分析結果顯示,FT水平與冠心病風險呈負相關(OR=0.98,95％CI:0.973～0.998,P=0.01),短AR增加冠心病髮病風險(OR=3.44,95％CI:1.887～6.264,P＜0.01). 結論 在老年男性中,雄激素受體CAG重複數短者,年齡相關的雄激素(主要是FT)水平下降更顯著,與冠心病髮病風險增加有關.
목적 탐토웅격소수체기인CAG중복수다태성대노년남성관심병발병풍험적영향급가능궤제. 방법 선택296례유관상동맥조영증거적노년남성,검측혈청중유리고동(FT)화총고동(TT)수평,류식세포술검측외주혈림파세포웅격소수체(AR)표체수평,병제취전혈기인조DNA,통과취합매련반응확증AR기인편단(함CAG중복수다태성위점),소득산물경미위성소묘분석화기인분형,최종득도상응적AR CAG중복수. 결과 노년남성환자적AR CAG중복수파동재11～28(P25～P75:18～22;중위수위20),이P75％위계분위장AR조[(CAG)n≥22,82례]화단AR조(CAG)n＜22,214례].량조비교,단AR조적FT수평저우장AR조(24.1±23.1)×10-6 mmol/L여(31.2±27.8)×10-6mmol/L (P＜0.05),단AR조합병관심병자FT수평저우대조조(22.4±20.5)×10-6mmol/L여(33.6±32.4)×10-6 mmol/L (P＜0.01)；이단AR조관심병발병솔84.1％ (180례)고우장AR조69.5％(57례)(P＜0.01).장AR조여단AR조적TT수평화림파세포AR표체수평차이무통계학의의.미발현AR기인(CAG)n중복수다태성대웅격소수평급림파세포표체수평유영향,년령시FT화AR수평적주요영향인소.Logistic회귀분석결과현시,FT수평여관심병풍험정부상관(OR=0.98,95％CI:0.973～0.998,P=0.01),단AR증가관심병발병풍험(OR=3.44,95％CI:1.887～6.264,P＜0.01). 결론 재노년남성중,웅격소수체CAG중복수단자,년령상관적웅격소(주요시FT)수평하강경현저,여관심병발병풍험증가유관.
Objective To investigate the relationship of androgen receptor (AR) CAG repeat polymorphism and coronary artery disease (CAD) in elderly men and its potential mechanism.Methods Totally 296 elderly men undergoing coronary angiography were enrolled in this study.Serum total testosterone (TT) and free testosterone (FT) levels were measured.Androgen receptors (ARs) in peripheral lymphocytes were determined by flow cytometry.Genome DNA was extracted from peripheral leucocytes using standard techniques.Gene fragments containing AR CAG repeats were amplified by PCR with specific fluorescent labeled primers.PCR products were separated with agarose gels.CAG repeat number of each sample was obtained by genotyping.Results AR CAG repeats varied from 11 to 28 (P25-P75:18-22; median:20) in elderly male patients.They were divided into the long AR group (CAG repeats≥22,n=82) and the short AR group (CAG repeats＜22,n=214).Compared with the long AR group,serum FT level was much lower in the short AR group [(24.1±23.1) ×10-6mmol/L vs.(31.2±27.8)×10-6mmol/L,P＜0.05].The prevalence of coronary artery disease was higher in the short AR group than in the long AR group [84.1％ (180 cases) vs.69.5％(57 cases),P＜0.05].The FT level was lower in the short AR group combined with CAD than in the control group [(22.4±20.5) ×10-6mmol/L vs.(33.6±32.4)×10 6mmol/L,P＜0.01].There were no significant differences in serum TT and AR levels between the long and short AR groups.No significant correlations were found in the AR CAG repeats polymorphism with FT,TT or AR levels.Age was the main risk factor for FT and AR levels.Logistic regression analysis showed that FT level was negatively correlated with CAD (OR=0.98,95 ％ CI:0.973-0.998,P=0.01),and short AR increased the risk of CAD in elderly male patients (OR=3.44,95％CI:1.887-6.264,P＜0.01).Conclusions Serum FT level is correlated with age and is significantly decreased in elderly male patients with short AR repeats,which may increase the risk of CAD in elderly men.
