中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2013年
7期
730-733
,共4页
管频%李伟%吴智勇%陈世良%吴洁
管頻%李偉%吳智勇%陳世良%吳潔
관빈%리위%오지용%진세량%오길
肺疾病,阻塞性%转化生长因子-β1%NF-κB
肺疾病,阻塞性%轉化生長因子-β1%NF-κB
폐질병,조새성%전화생장인자-β1%NF-κB
Lung diseases,obstructive%Transforming growth factor beta-1%NF-kappa B
目的 观察转化生长因子-β1(TGF-β1)、核转录因子κB (NF-κB)在慢性阻塞性肺疾病(COPD)肺组织的表达及与肺功能指标的相关性,探讨COPD的发病机制. 方法 收集因肺部肿瘤行肺叶切除术患者38例,其中未达到COPD诊断标准19例(对照组),平均年龄(61.7±8.8)岁,符合COPD诊断标准患者19例(COPD组),平均年龄(60.5±9.4)岁,取其行肺叶切除术后的远离肿瘤病灶的外周肺组织.用免疫组化方法及Western blot法分别检测所取肺组织中NF-κB的定性及定量表达.反转录聚合酶链反应(RT-PCR)方法检测所取肺组织中TGF-β1的mRNA半定量表达.结果 (1) COPD组TGF-β31mRNA相对表达量、NF-κB蛋白质相对表达量及NF-κB胞核阳性率分别为0.42±0.11、0.24±0.08和57.9％,均明显高于对照组(0.34±0.12、0.12±0.04和26.7％),差异均有统计学意义(均P＜0.05).(2) NF-κB、TGF-β1mRNA表达的相关性:两组NF-κB蛋白表达与TGF-β1mRNA表达均呈正相关(r=0.497、0.618,P＜0.01)；(3)COPD组中1秒用力呼气容积/用力肺活量(FEV1/ FVC)百分比分别与TGF-β1mRNA表达、NF-κB蛋白质表达量呈负相关(r=-0.624、-0.659,均P＜0.01). 结论 NF-κB与TGF-β1的表达在COPD外周肺组织中明显升高,且两者呈正相关；NFκB可能参与了TGF-β1的基因调控,从而参与并促进气道重塑,进而影响肺功能.
目的 觀察轉化生長因子-β1(TGF-β1)、覈轉錄因子κB (NF-κB)在慢性阻塞性肺疾病(COPD)肺組織的錶達及與肺功能指標的相關性,探討COPD的髮病機製. 方法 收集因肺部腫瘤行肺葉切除術患者38例,其中未達到COPD診斷標準19例(對照組),平均年齡(61.7±8.8)歲,符閤COPD診斷標準患者19例(COPD組),平均年齡(60.5±9.4)歲,取其行肺葉切除術後的遠離腫瘤病竈的外週肺組織.用免疫組化方法及Western blot法分彆檢測所取肺組織中NF-κB的定性及定量錶達.反轉錄聚閤酶鏈反應(RT-PCR)方法檢測所取肺組織中TGF-β1的mRNA半定量錶達.結果 (1) COPD組TGF-β31mRNA相對錶達量、NF-κB蛋白質相對錶達量及NF-κB胞覈暘性率分彆為0.42±0.11、0.24±0.08和57.9％,均明顯高于對照組(0.34±0.12、0.12±0.04和26.7％),差異均有統計學意義(均P＜0.05).(2) NF-κB、TGF-β1mRNA錶達的相關性:兩組NF-κB蛋白錶達與TGF-β1mRNA錶達均呈正相關(r=0.497、0.618,P＜0.01)；(3)COPD組中1秒用力呼氣容積/用力肺活量(FEV1/ FVC)百分比分彆與TGF-β1mRNA錶達、NF-κB蛋白質錶達量呈負相關(r=-0.624、-0.659,均P＜0.01). 結論 NF-κB與TGF-β1的錶達在COPD外週肺組織中明顯升高,且兩者呈正相關；NFκB可能參與瞭TGF-β1的基因調控,從而參與併促進氣道重塑,進而影響肺功能.
목적 관찰전화생장인자-β1(TGF-β1)、핵전록인자κB (NF-κB)재만성조새성폐질병(COPD)폐조직적표체급여폐공능지표적상관성,탐토COPD적발병궤제. 방법 수집인폐부종류행폐협절제술환자38례,기중미체도COPD진단표준19례(대조조),평균년령(61.7±8.8)세,부합COPD진단표준환자19례(COPD조),평균년령(60.5±9.4)세,취기행폐협절제술후적원리종류병조적외주폐조직.용면역조화방법급Western blot법분별검측소취폐조직중NF-κB적정성급정량표체.반전록취합매련반응(RT-PCR)방법검측소취폐조직중TGF-β1적mRNA반정량표체.결과 (1) COPD조TGF-β31mRNA상대표체량、NF-κB단백질상대표체량급NF-κB포핵양성솔분별위0.42±0.11、0.24±0.08화57.9％,균명현고우대조조(0.34±0.12、0.12±0.04화26.7％),차이균유통계학의의(균P＜0.05).(2) NF-κB、TGF-β1mRNA표체적상관성:량조NF-κB단백표체여TGF-β1mRNA표체균정정상관(r=0.497、0.618,P＜0.01)；(3)COPD조중1초용력호기용적/용력폐활량(FEV1/ FVC)백분비분별여TGF-β1mRNA표체、NF-κB단백질표체량정부상관(r=-0.624、-0.659,균P＜0.01). 결론 NF-κB여TGF-β1적표체재COPD외주폐조직중명현승고,차량자정정상관；NFκB가능삼여료TGF-β1적기인조공,종이삼여병촉진기도중소,진이영향폐공능.
Objective To investigate the expressions of transforming growth factor beta-1 (TGF-β1) and nuclear factor kappa B (NF-κB) in patients with chronic obstructive pulmonary disease (COPD),and to explore the mechanism of pathogenesis in COPD.Methods A total of 40 patients undergoing lung resections for pulmonary tumor were selected.Patients were divided into 2 groups according to COPD diagnostic criteria:the control group [patients without COPD,13 males,7 females,with an average age of (61.7±8.8) years] and the COPD group [patients with COPD,15 males,5 females,with an average age of (60.5 ± 9.4) years].Peripheral lung tissues from tumor lesions were detected in this study.The qualitative and quantitative expressions of NF-κB were determined by immunohistochemistry and Western blot,respectively.TGF-β31 mRNA level was determined by reverse transcription polymerase chain reaction (RT-PCR).Results The levels of TGF β1 mRNA and NF-κB protein and the NF-κB nucleus positive rate were significantly higher in the COPDgroup than in the control group [(0.42±0.11) vs.(0.34±0.13),(0.24±0.08) vs.(0.12±0.04),57.9％ vs.26.7％,respectively,all P＜0.05].The TGF-β31 mRNA level was positively correlated with the NF-κB protein expression in the 2 groups (r=0.497,0.618,both P＜0.01).The ratio of 1 second forced expiratory volume/forced vital capacity (FEV1/ FVC) was negatively correlated with TGF-β1 mRNA level and NF-κB protein expression (r=-0.624,r=-0.659,both P ＜0.01) in the COPD group.Conclusions The expression levels of NF-κB and TGF-β1 are significantly increased in patients with COPD,and there is a positive correlation between TGF-β1mRNA level and NF-κB protein expression.NF-κB may participate in regulating TGF-β1 mRNA expression and in contributing to the airway remodeling,thereby in effecting pulmonary function.
