CAJ | 학술논문

目的研究老年人多原发与单发膀胱癌中B细胞淋巴瘤/白血病-2(bcl-2)、表皮生长因子受体(EGFR)、C-erbB-2基因的表达. 方法运用免疫组化方法检测1996年至2012年间收治经病理确诊的老年多原发和单发膀胱癌各20例(临床分期、病理分级与老年多原发膀胱癌相同),老年正常膀胱组织15例,3组共55例中bcl-2、EGFR、C-erbB-2的平均透光度值,比较其阳性表达的差异性. 结果老年多原发及单发膀胱癌中bcl-2、EGFR、C-erbB-2的阳性表达均高于老年正常膀胱组织,bcl 2、EGFR、C-erbB-2平均透光度值多发膀胱癌组织分别为80.11±7.93,118.35±11.84,96.69±12.53；单发膀胱癌组织分别为84.06±6.91,102.73±10.92,89.14±9.84;正常组织分别为92.67±6.56,147.53±11.76,105.88±9.86(均P＜0.01),老年多原发与老年单发膀胱癌组织中bcl-2、EGFR、C-erbB-2表达差异也有统计学意义(P＜0.05或P＜0.01),老年多原发膀胱癌中bcl-2的阳性表达率高于老年单发膀胱癌(P＜0.05),老年多原发膀胱癌中EGFR、C-erbB-2的阳性表达均低于老年单发膀胱癌(均P＜0.01). 结论 bcl-2、EGFR、C-erbB-2在老年多原发与单发膀胱癌中表达有差异性,联合检测bcl-2、EGFR、C-erbB-2在诊断和鉴别老年多原发膀胱癌中具有一定临床价值.本研究也为老年多原发膀胱癌靶向治疗提供了可能.
목적 연구노년인다원발여단발방광암중B세포림파류/백혈병-2(bcl-2)、표피생장인자수체(EGFR)、C-erbB-2기인적표체. 방법 운용면역조화방법검측1996년지2012년간수치경병리학진적노년다원발화단발방광암각20례(림상분기、병리분급여노년다원발방광암상동),노년정상방광조직15례,3조공55례중bcl-2、EGFR、C-erbB-2적평균투광도치,비교기양성표체적차이성. 결과 노년다원발급단발방광암중bcl-2、EGFR、C-erbB-2적양성표체균고우노년정상방광조직,bcl 2、EGFR、C-erbB-2평균투광도치다발방광암조직분별위80.11±7.93,118.35±11.84,96.69±12.53；단발방광암조직분별위84.06±6.91,102.73±10.92,89.14±9.84;정상조직분별위92.67±6.56,147.53±11.76,105.88±9.86(균P＜0.01),노년다원발여노년단발방광암조직중bcl-2、EGFR、C-erbB-2표체차이야유통계학의의(P＜0.05혹P＜0.01),노년다원발방광암중bcl-2적양성표체솔고우노년단발방광암(P＜0.05),노년다원발방광암중EGFR、C-erbB-2적양성표체균저우노년단발방광암(균P＜0.01). 결론 bcl-2、EGFR、C-erbB-2재노년다원발여단발방광암중표체유차이성,연합검측bcl-2、EGFR、C-erbB-2재진단화감별노년다원발방광암중구유일정림상개치.본연구야위노년다원발방광암파향치료제공료가능.
Objective To study the expressions of B cell lymphoma/leukemia-2 (bcl-2),epidermal growth factor receptor (EGFR) and c-erbB-2 in elderly patients with multiple primary and single bladder cancer.Methods From 1996 to 2012,40 patients pathologically diagnosed as bladder cancer were treated in our hospital,and were divided into 2 groups:group 1(n=20,patients with multiple primary bladder cancer) and group 2 (n=20,patients with single bladder cancer).15 elderly people with normal bladder were selected as control group.The expressions of bcl-2,EGFR and c-erbB-2 were detected by immunohistochemical SP method and compared between groups.Results The positive expressions of bcl-2,EGFR and c-erbB-2 were significantly higher in group 1 and group 2 than in control group (average transparency value:80.11±7.93,84.06±6.91 vs.92.67±6.56; 118.35±11.84,102.73±10.92 vs.147.53±11.76; 96.69±12.53,89.14±9.84 vs.105.88±9.86; respectively,all P＜0.01).The positive expression of bcl-2 was higher in group 1 than in group 2 (P＜0.05).The positive expressions of EGFR and c-erbB-2 were lower in group 1 than in group 2 (P＜ 0.01).Conclusions The positive expressions of bcl-2,EGFR and c-erbB-2 have significant differences between elderly patients with multiple primary and single bladder cancer.The combined detection of bcl-2,EGFR and c-erbB-2 has the clinical value in diagnosis and identification of multiple primary bladder cancer in elderly patients.This study may provide the possibility of targeting therapy for multiple primary bladder cancer in elderly patients.