中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2013年
9期
1006-1009
,共4页
吴克芬%李希%任卫英%胡予
吳剋芬%李希%任衛英%鬍予
오극분%리희%임위영%호여
半乳糖%衰老%肠黏膜%连接蛋白类
半乳糖%衰老%腸黏膜%連接蛋白類
반유당%쇠로%장점막%련접단백류
Galactose%Aging%Intestinal mucosa%Connexins
目的 了解大鼠肠道上皮屏障功能随增龄所发生的变化. 方法 选用3月龄组、12月龄组、24月龄衰老模型鼠组,每组各10只.取大鼠末端回肠制作切片观察小肠黏膜形态学变化及绒毛高度、厚度等.采用免疫组化Envision两步法、反转录-聚合酶链反应(RT-PCR)方法检测大鼠末端回肠黏膜的紧密连接蛋白闭锁蛋白(Occludin)、闭锁小带蛋白(ZO-1)的分布和表达. 结果 24月龄衰老模型鼠、12月龄大鼠小肠黏膜厚度及绒毛高度均较3月龄降低[小肠黏膜厚度:24月龄(87.6±6.32)μm,12月龄(131.8±5.22) μm,3月龄(162.9±7.28)μm,P＜0.05;绒毛高度:24月龄(56.4±5.38)μm,12月龄(76.7±5.40)μm,3月龄(108.1±6.42)μm,P＜0.05],且24月龄衰老模型鼠小肠黏膜厚度及绒毛高度较12月龄亦降低(P＜0.05).24月龄衰老模型鼠、12月龄大鼠小肠黏膜组织中紧密连接蛋白(Occludin、ZO-1)表达均较3月龄减少[Occludin蛋白:24月龄(2.23±0.60)％,12月龄(4.21±0.61)％,3月龄(12.31±0.94)％,P＜0.05; ZO-1蛋白:24月龄(2.03±0.54)％,12月龄(4.02±0.65)％,3月龄(12.21±0.81)％,P＜0.05],24月龄衰老模型鼠小肠黏膜组织中紧密连接蛋白(Occludin、ZO-1)表达较12月龄亦降低(P＜0.05).24月龄衰老模型鼠和12月龄大鼠小肠黏膜组织中Occludin mRNA和ZO-1mRNA相对表达量较3月龄减少(OccludinmRNA:24月龄0.20±0.03,12月龄0.38±0.02,3月龄0.66±0.03,P＜0.05；ZO-1mRNA:24月龄0.18±0.03,12月龄0.37±0.02,3月龄0.63±0.03,P＜0.05),24月龄衰老模型组与12月龄组比较差异有统计学意义(P＜0.05). 结论 随着年龄的增长大鼠小肠黏膜厚度及绒毛高度逐渐降低,小肠黏膜的紧密连接蛋白逐渐减少,小肠黏膜屏障功能受损.
目的 瞭解大鼠腸道上皮屏障功能隨增齡所髮生的變化. 方法 選用3月齡組、12月齡組、24月齡衰老模型鼠組,每組各10隻.取大鼠末耑迴腸製作切片觀察小腸黏膜形態學變化及絨毛高度、厚度等.採用免疫組化Envision兩步法、反轉錄-聚閤酶鏈反應(RT-PCR)方法檢測大鼠末耑迴腸黏膜的緊密連接蛋白閉鎖蛋白(Occludin)、閉鎖小帶蛋白(ZO-1)的分佈和錶達. 結果 24月齡衰老模型鼠、12月齡大鼠小腸黏膜厚度及絨毛高度均較3月齡降低[小腸黏膜厚度:24月齡(87.6±6.32)μm,12月齡(131.8±5.22) μm,3月齡(162.9±7.28)μm,P＜0.05;絨毛高度:24月齡(56.4±5.38)μm,12月齡(76.7±5.40)μm,3月齡(108.1±6.42)μm,P＜0.05],且24月齡衰老模型鼠小腸黏膜厚度及絨毛高度較12月齡亦降低(P＜0.05).24月齡衰老模型鼠、12月齡大鼠小腸黏膜組織中緊密連接蛋白(Occludin、ZO-1)錶達均較3月齡減少[Occludin蛋白:24月齡(2.23±0.60)％,12月齡(4.21±0.61)％,3月齡(12.31±0.94)％,P＜0.05; ZO-1蛋白:24月齡(2.03±0.54)％,12月齡(4.02±0.65)％,3月齡(12.21±0.81)％,P＜0.05],24月齡衰老模型鼠小腸黏膜組織中緊密連接蛋白(Occludin、ZO-1)錶達較12月齡亦降低(P＜0.05).24月齡衰老模型鼠和12月齡大鼠小腸黏膜組織中Occludin mRNA和ZO-1mRNA相對錶達量較3月齡減少(OccludinmRNA:24月齡0.20±0.03,12月齡0.38±0.02,3月齡0.66±0.03,P＜0.05；ZO-1mRNA:24月齡0.18±0.03,12月齡0.37±0.02,3月齡0.63±0.03,P＜0.05),24月齡衰老模型組與12月齡組比較差異有統計學意義(P＜0.05). 結論 隨著年齡的增長大鼠小腸黏膜厚度及絨毛高度逐漸降低,小腸黏膜的緊密連接蛋白逐漸減少,小腸黏膜屏障功能受損.
목적 료해대서장도상피병장공능수증령소발생적변화. 방법 선용3월령조、12월령조、24월령쇠로모형서조,매조각10지.취대서말단회장제작절편관찰소장점막형태학변화급융모고도、후도등.채용면역조화Envision량보법、반전록-취합매련반응(RT-PCR)방법검측대서말단회장점막적긴밀련접단백폐쇄단백(Occludin)、폐쇄소대단백(ZO-1)적분포화표체. 결과 24월령쇠로모형서、12월령대서소장점막후도급융모고도균교3월령강저[소장점막후도:24월령(87.6±6.32)μm,12월령(131.8±5.22) μm,3월령(162.9±7.28)μm,P＜0.05;융모고도:24월령(56.4±5.38)μm,12월령(76.7±5.40)μm,3월령(108.1±6.42)μm,P＜0.05],차24월령쇠로모형서소장점막후도급융모고도교12월령역강저(P＜0.05).24월령쇠로모형서、12월령대서소장점막조직중긴밀련접단백(Occludin、ZO-1)표체균교3월령감소[Occludin단백:24월령(2.23±0.60)％,12월령(4.21±0.61)％,3월령(12.31±0.94)％,P＜0.05; ZO-1단백:24월령(2.03±0.54)％,12월령(4.02±0.65)％,3월령(12.21±0.81)％,P＜0.05],24월령쇠로모형서소장점막조직중긴밀련접단백(Occludin、ZO-1)표체교12월령역강저(P＜0.05).24월령쇠로모형서화12월령대서소장점막조직중Occludin mRNA화ZO-1mRNA상대표체량교3월령감소(OccludinmRNA:24월령0.20±0.03,12월령0.38±0.02,3월령0.66±0.03,P＜0.05；ZO-1mRNA:24월령0.18±0.03,12월령0.37±0.02,3월령0.63±0.03,P＜0.05),24월령쇠로모형조여12월령조비교차이유통계학의의(P＜0.05). 결론 수착년령적증장대서소장점막후도급융모고도축점강저,소장점막적긴밀련접단백축점감소,소장점막병장공능수손.
Objective To study the changes of intestinal epithelial barrier function in rats with aging.Methods SD rats were divided into 3 groups:3-month-old group (group A),12-month-old group (group B) and 24-month-old group (group C,established by D-galactose injection with the dose of 0.125 g· kg-1 · d-1subcultaneously for 6 weeks) (n=10,each).The terminal ileum was obtained to make microtome section,and the morphology of small intestine mucous membrane,trophonema altitude and thickness were observed under light microscope.Occludin and ZO-1 protein expressions in terminal ileum mucous membrane were detected by immunohistochemistry.The expressions of Occludin and ZO 1 mRNA were determined by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR).Results The small intestinal mucosa thickness and villus height were lower in group C and B than in group A [thickness:(87.6± 6.32) μm,(131.8± 5.22) μm vs.(162.9±7.28) μm; villus height:(56.4±5.38) μm,(76.7±5.40) μm vs.(108.1±6.42) μm;both P＜0.05].The small intestinal mucosa thickness and villus height was lower in group C than in group B (both P＜0.05).Occludin and ZO-1 protein expressions in small intestine tissue were reduced in group C and B as compared with group A [Occludin protein:(2.23±0.60)％,(4.21±0.61)％ vs.(12.31±0.94)％; ZO-1 protein:(2.03±0.54)％,(4.02±0.65) ％ vs.(12.21±0.81)％ ; both P＜0.05],and Occludin and ZO-1 protein expressions were less in group C than in group B (both P＜0.05).The levels of Occludin and ZO-1 mRNA in small intestine tissue were reduced in group C and B as compared with group A [Occludin:(0.20±0.03),(0.38±0.02) vs.(0.66±0.03) ; ZO-1:(0.18±0.03),(0.37±0.02) vs.(0.63±0.03); both P＜0.05],and Occludin and ZO-1 mRNA expressions were less in group C than in group B (both P ＜ 0.05).Conclusions The small intestinal mucosa thickness and villus height are reduced,the levels of Occludin and ZO-1 expressions are significantly decreased in small intestinal mucosa,and the intestinal barrier function is impaired with rat aging.
