CAJ | 학술논문

目的探讨胰岛素抵抗(IR)大鼠在心肌缺血状态下心肌Apelin、APJ表达变化. 方法 24只雄性SD大鼠随机分IR组、IR+缺血组、对照组,各8只.IR组及IR+缺血组予高脂饲料喂养.对照组予以基础饲料喂养.判断IR造模成功后,给IR+缺血组皮下注射异丙基肾上腺素1mg/d/kg,连续7d,制造缺血模型,其余两组注射等量生理盐水.取心肌组织real-time-PCR法测定APJ和Apelin mRNA表达水平.免疫组化测定Apelin多肽的阳性表达. 结果 Apelin:IR组较对照组表达升高(0.16±0.004比0.13±0.005,P＜0.05)；IR+缺血组较对照组(0.10±0.002比0.13±0.005,P＜0.05)及IR组(0.10±0.002比0.16±0.004,P＜0.05)表达均明显降低.ApelinmRNA相对表达量比较:IR组较对照组表达量升高(5.89±0.36比4.40±0.24,P＜0.05),IR+缺血组较IR组(5.89±0.36比2.66±0.17,P＜0.05)相对表达量均明显降低.APJ mRNA相对表达量:IR组较对照组相对表达量明显升高(10.46±1.06比6.54±0.63,P＜0.05)；IR+缺血组较IR组相对表达量降低(3.31±0.31比10.46±1.06,P＜0.05). 结论胰岛素抵抗大鼠中Apelin、APJ可能对心脏发挥保护作用,在心肌缺血时可反馈性抑制Apelin、APJ表达,减弱了对心脏的保护作用.
목적 탐토이도소저항(IR)대서재심기결혈상태하심기Apelin、APJ표체변화. 방법 24지웅성SD대서수궤분IR조、IR+결혈조、대조조,각8지.IR조급IR+결혈조여고지사료위양.대조조여이기출사료위양.판단IR조모성공후,급IR+결혈조피하주사이병기신상선소1mg/d/kg,련속7d,제조결혈모형,기여량조주사등량생리염수.취심기조직real-time-PCR법측정APJ화Apelin mRNA표체수평.면역조화측정Apelin다태적양성표체. 결과 Apelin:IR조교대조조표체승고(0.16±0.004비0.13±0.005,P＜0.05)；IR+결혈조교대조조(0.10±0.002비0.13±0.005,P＜0.05)급IR조(0.10±0.002비0.16±0.004,P＜0.05)표체균명현강저.ApelinmRNA상대표체량비교:IR조교대조조표체량승고(5.89±0.36비4.40±0.24,P＜0.05),IR+결혈조교IR조(5.89±0.36비2.66±0.17,P＜0.05)상대표체량균명현강저.APJ mRNA상대표체량:IR조교대조조상대표체량명현승고(10.46±1.06비6.54±0.63,P＜0.05)；IR+결혈조교IR조상대표체량강저(3.31±0.31비10.46±1.06,P＜0.05). 결론 이도소저항대서중Apelin、APJ가능대심장발휘보호작용,재심기결혈시가반궤성억제Apelin、APJ표체,감약료대심장적보호작용.
Objective To investigate the expression of apelin and its recoptor (APJ) in myocardium in insulin-resistant CIR rats with myocardial ischemia.Methods Totally 24 male SD rats were randomly divided into three groups:IR group,IR+ischemia group,the control group (n=8 each).Rats in IR and IR+ischemia groups were fed with the high fat diet.Rats in control group were given the basic diet.The rat model of insulin resistance was assessed by fasting blood glucose (FBG),fasting insulin (Fins) and insulin resistance index (HOMA-IR).The rat model of myocardial ischemia was conducted by subcutaneous injection of isoprenaline 1 mg/kg per day in IR+ ischemia group.The other groups were injected an equal volume of saline.The expression levels of apelin and APJ mRNA in myocardium were determined by real-time-PCR.The positive expression of apelin polypeptide was detected by immunochemistry.Results Compared with the control group,the apelin average optical density was increased in IR group and was decreased in IR+ ischemia group [(0.16±0.004) vs.(0.13±0.005),(0.10±0.002) vs.(0.13±0.0050),both P＜0.05].There was a significant difference in apelin average optical density between IR group and IR+ischemia group [(0.16± 0.004) vs.(0.10±0.002),P＜0.05].Compared with the control group,the relative expression of apelin mRNA was increased in IR group [(5.89±0.36) vs.(4.40±0.24),P＜0.05]The relative expression of apelin mRNA was decreased in IR± ischemia group as compared with IR group [(2.66 ± 0.17) vs.(5.89 ± 0.36),P ＜ 0.05].The APJ mRNA relative expression was increased in IR group as compared with control group and was decreased in IR+ ischemia group as compared with IR group [(10.46±1.06) vs.(6.54±0.63),(3.31±0.31) vs.(10.46±1.06),both P＜0.05].Conclusions The expressions of apelin and APJ are inhibited in insulin-resistant rats with myocardial ischemia,which attenuates their protective effects on myocardium.