CAJ | 학술논문

目的探讨广西巴马地区壮族长寿家系口腔黏膜脱落上皮细胞端粒的长度及其潜在意义. 方法应用实时荧光定量PCR方法测定巴马地区长寿家系(1250例)口腔黏膜脱落细胞的相对端粒长度,并与当地非长寿家系(556例)及外地区(广西平果)长寿家系(630例)比较. 结果 (1)在所有的研究人群(2436例)中,口腔黏膜脱落细胞端粒长度均与年龄呈负相关(R =-0.215,P=0.000),即年龄越大,端粒长度越短,而且这种变化不受性别的影响；(2)在巴马地区长寿家系中,各年龄段男性和女性的端粒长度均差异无统计学意义(均P＞0.05),其他家系除个别年龄段外,也表现为类似的趋势；(3)巴马地区长寿家系65～年龄段(相当于长寿老人的第一胎子女)的端粒长度长于非长寿家系(1.969比1.622,P=0.004),同时,90～高龄段的端粒长度与非长寿家系65～年龄段的水平相当(1.662比1.622,P=0.955). 结论巴马地区长寿家系口腔细胞的端粒长度随年龄的增大而缩短；端粒的长短有一定的遗传倾向,可能是该地区长寿的机制之一.
목적 탐토엄서파마지구장족장수가계구강점막탈락상피세포단립적장도급기잠재의의. 방법 응용실시형광정량PCR방법측정파마지구장수가계(1250례)구강점막탈락세포적상대단립장도,병여당지비장수가계(556례)급외지구(엄서평과)장수가계(630례)비교. 결과 (1)재소유적연구인군(2436례)중,구강점막탈락세포단립장도균여년령정부상관(R =-0.215,P=0.000),즉년령월대,단립장도월단,이차저충변화불수성별적영향；(2)재파마지구장수가계중,각년령단남성화녀성적단립장도균차이무통계학의의(균P＞0.05),기타가계제개별년령단외,야표현위유사적추세；(3)파마지구장수가계65～년령단(상당우장수노인적제일태자녀)적단립장도장우비장수가계(1.969비1.622,P=0.004),동시,90～고령단적단립장도여비장수가계65～년령단적수평상당(1.662비1.622,P=0.955). 결론 파마지구장수가계구강세포적단립장도수년령적증대이축단；단립적장단유일정적유전경향,가능시해지구장수적궤제지일.
Objective To explore the telomere length (TL) in buccal cells and its possible implications for long-lived families of Zhuang nationality in Bama area of Guangxi.Methods Relative TL in buccal cells from Bama long-lived families (BLF,n=1250) was determined by real time PCR and compared between Bama non-long-lived families (BNLF,n=556) and Pingguo longlived families (PLF,n=630).All participants were Zhuang ethnic.Results The TL in buccal cells was negatively correlated with age (R=-0.215,P=0.000) and was independent of sex in all subjects (n=2436).There were no significant differences in TL between males and females in different of ages in BLF (all P＞0.05).Similar tendency was observed in most but not all age groups in the two other families.The TL was significantly longer in BLF aged over 65 years (the first offspring of the longlived individual) than in BNLF aged over 65 years (1.969 vs.1.622,P=0.004) and the TL of BLF aged over 90 years was comparable to that of BNLF aged over 65 years (1.662 vs.1.622,P=0.955),which indicating that the offspring of long-lived individuals inherited longer TL from their parental generation.Conclusions Telomere length is shorten with aging in long-lived families in Bama area.The TL of BLF has a tendency of inheritance,which may be one of the mechanisms of longevity in Bama area.