CAJ | 학술논문

目的用荧光定量聚合酶链式反应(qRT-PCR)方法检测急性心肌梗死(AMI)患者血浆中的MicroRNAs水平,评价其在AMI早期诊断中的价值. 方法选取AMI患者24例,对照组20例为研究对象,分别在症状发作后3h、6h、12 h、24 h、48 h、72 h检测血浆中microRNA-1、microRNA-133a、microRNA-208a、microRNA-499的水平. 结果 miRNA-1在缺血性胸痛发作后3 h血浆水平明显升高达到高峰,72 h基本降至正常；miRNA-133a在6h后血浆水平明显升高,12h达到高峰,48 h降至正常;miRNA-1和miRNA-133a表达水平与心肌肌钙蛋白I(cTnI)相关,miRNA-1的峰值出现在cTnI之前,miRNA-133a峰值与cTnI发生在同一时间. 结论循环中升高的miRNA-1、miRNA-133a可能是诊断早期AMI的一种有潜力的、新型的生物标志物.
목적 용형광정량취합매련식반응(qRT-PCR)방법검측급성심기경사(AMI)환자혈장중적MicroRNAs수평,평개기재AMI조기진단중적개치. 방법 선취AMI환자24례,대조조20례위연구대상,분별재증상발작후3h、6h、12 h、24 h、48 h、72 h검측혈장중microRNA-1、microRNA-133a、microRNA-208a、microRNA-499적수평. 결과 miRNA-1재결혈성흉통발작후3 h혈장수평명현승고체도고봉,72 h기본강지정상；miRNA-133a재6h후혈장수평명현승고,12h체도고봉,48 h강지정상;miRNA-1화miRNA-133a표체수평여심기기개단백I(cTnI)상관,miRNA-1적봉치출현재cTnI지전,miRNA-133a봉치여cTnI발생재동일시간. 결론 순배중승고적miRNA-1、miRNA-133a가능시진단조기AMI적일충유잠력적、신형적생물표지물.
Objective To evaluate the importance of plasma MicroRNAs in early diagnosis of acute myocardial infarction (AMI).Methods 24 patients with AMI as the test group and 20healthy volunteers as the control group were enrolled in this study.Plasma levels of microRNA-1,microRNA-133a,microRNA-208a and microRNA-499 were detected by quantitative real time polymerase chain reaction (qRT-PCR) at 3 h,6 h,12 h,24 h,48 h,72 h after the onset of AMI.Results Plasma microRNA-1 level was greatly increased and reached the peak at 3 h after AMI,then was decreased gradually to normal level at 72 h after AMI.Plasma microRNA-133a level was significantly elevated at 6 h after AMI,reached peak at 12 h after AMI,then was decreased to normal level at 48 h after AMI.Plasma microRNA-1 and microRNA-133a levels were correlated with cTnI expression.The peak time of microRNA-1 was earlier than that of cTnI,while the peak time of microRNA-133a was the same as that of cTnI.Conclusions Increased circulating microRNA-1 and microRNA-133a may serve as potential and novel biomarkers for early diagnosis of AMI.