中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2013年
11期
1228-1232
,共5页
黄树其%杨月嫦%黄流清%邵福源
黃樹其%楊月嫦%黃流清%邵福源
황수기%양월항%황류청%소복원
痴呆,血管性%红细胞生成素%认知%细胞凋亡
癡呆,血管性%紅細胞生成素%認知%細胞凋亡
치태,혈관성%홍세포생성소%인지%세포조망
Dementia,vascular%Erythropoietin%Cognition%Apoptosis
目的 探讨促红细胞生成素(EPO)对血管性痴呆(VaD)大鼠认知功能及其海马CA1区神经细胞凋亡的影响. 方法 将54只Wistar大鼠分为假手术组、VaD组和EPO组,采用永久性结扎双侧颈总动脉的方法建立VaD模型.采用y-迷宫观察术后4周、8周、12周大鼠的空间学习和记忆能力,应用TUNEL染色检测大鼠海马CA1的神经细胞凋亡数,通过免疫组织化学和逆转录聚合酶链反应检测大鼠海马CA1区Bcl-2和Bax mRNA表达水平的变化. 结果 与VaD组大鼠比较,术后4周、8周、12周EPO治疗组大鼠迷宫作业错误反应次数明显减少,总反应时间明显缩短(均P＜0.05);术后4周、8周和12周VaD组大鼠海马CA1区凋亡细胞数目分别为(20.50±3.29)个、(33.58±3.48)个和(54.17±4.26)个,比EPO治疗组的(10.50±2.43)个、(23.92±3.18)个和(36.92±4.10)个多(t=4.23、3.54、5.05,P=0.013、0.024、0.007);术后4周、8周和12周EPO治疗组Bc[-2阳性细胞数和Bcl-2 mRNA表达较VaD组明显增多,Bax阳性细胞数和Bax mRNA表达较VaD组明显减少(均P＜0.05). 结论 EPO可能通过调控VaD大鼠海马CA1区Bcl-2和Bax表达抑制神经细胞凋亡,改善其认知功能障碍.
目的 探討促紅細胞生成素(EPO)對血管性癡呆(VaD)大鼠認知功能及其海馬CA1區神經細胞凋亡的影響. 方法 將54隻Wistar大鼠分為假手術組、VaD組和EPO組,採用永久性結扎雙側頸總動脈的方法建立VaD模型.採用y-迷宮觀察術後4週、8週、12週大鼠的空間學習和記憶能力,應用TUNEL染色檢測大鼠海馬CA1的神經細胞凋亡數,通過免疫組織化學和逆轉錄聚閤酶鏈反應檢測大鼠海馬CA1區Bcl-2和Bax mRNA錶達水平的變化. 結果 與VaD組大鼠比較,術後4週、8週、12週EPO治療組大鼠迷宮作業錯誤反應次數明顯減少,總反應時間明顯縮短(均P＜0.05);術後4週、8週和12週VaD組大鼠海馬CA1區凋亡細胞數目分彆為(20.50±3.29)箇、(33.58±3.48)箇和(54.17±4.26)箇,比EPO治療組的(10.50±2.43)箇、(23.92±3.18)箇和(36.92±4.10)箇多(t=4.23、3.54、5.05,P=0.013、0.024、0.007);術後4週、8週和12週EPO治療組Bc[-2暘性細胞數和Bcl-2 mRNA錶達較VaD組明顯增多,Bax暘性細胞數和Bax mRNA錶達較VaD組明顯減少(均P＜0.05). 結論 EPO可能通過調控VaD大鼠海馬CA1區Bcl-2和Bax錶達抑製神經細胞凋亡,改善其認知功能障礙.
목적 탐토촉홍세포생성소(EPO)대혈관성치태(VaD)대서인지공능급기해마CA1구신경세포조망적영향. 방법 장54지Wistar대서분위가수술조、VaD조화EPO조,채용영구성결찰쌍측경총동맥적방법건립VaD모형.채용y-미궁관찰술후4주、8주、12주대서적공간학습화기억능력,응용TUNEL염색검측대서해마CA1적신경세포조망수,통과면역조직화학화역전록취합매련반응검측대서해마CA1구Bcl-2화Bax mRNA표체수평적변화. 결과 여VaD조대서비교,술후4주、8주、12주EPO치료조대서미궁작업착오반응차수명현감소,총반응시간명현축단(균P＜0.05);술후4주、8주화12주VaD조대서해마CA1구조망세포수목분별위(20.50±3.29)개、(33.58±3.48)개화(54.17±4.26)개,비EPO치료조적(10.50±2.43)개、(23.92±3.18)개화(36.92±4.10)개다(t=4.23、3.54、5.05,P=0.013、0.024、0.007);술후4주、8주화12주EPO치료조Bc[-2양성세포수화Bcl-2 mRNA표체교VaD조명현증다,Bax양성세포수화Bax mRNA표체교VaD조명현감소(균P＜0.05). 결론 EPO가능통과조공VaD대서해마CA1구Bcl-2화Bax표체억제신경세포조망,개선기인지공능장애.
Objective To explore the effect of erythropoietin (EPO) on cognition and neuron apoptosis in CA1 region of hippocampus in vascular dementia (VaD) rats.Methods 54 Wistar rats were randomly divided into three groups:sham control group,VaD group,VaD+ EPO group (n=18,each).The bilateral common carotid arteries of Wistar rats were permanently ligated to establish VaD models.Spatial study and memory were observed by Y maze test at 4,8 and 12 weeks after operation.Neuron apoptosis in CA1 area of hippocampus was measured by terminal deoxynucleotidyl-transferase (TdT) mediated dUTP-biotin nick end labeling (TUNEL) method.The protein and mRNA expressions of Bcl 2 and Bax in CA1 region of hippocampus were detected by immunohistochemistry and RT-PCR at 4,8,12 weeks after operation.Results Compared with VaD group,the VaD+ EPO group had better performances including less error reaction times and shorter total reaction time in Y-maze (both P＜0.05) at 4,8,12 weeks after operation.The apoptotic neuronal cells in CA1 region of hippocampus was decreased in VaD+ EPO group than in VaD group at 4,8,12 weeks after operation [(10.50±±2.43) vs.(20.50±± 3.29),(23.92±±3.18) vs.(33.58±3.48) and (36.92±4.10) vs.(54.17±4.26),t=4.23,3.54,5.05,P=0.013,0.024,0.007,respectively].The Bcl-2-positive cells and Bcl-2 mRNA expression were increased,and the Baxpositive cells and Bax mRNA expression were decreased in VaD+ EPO group than in VaD group (all P ＜0.05).Conclusions Erythropoietin can improve cognitive function by inhibiting neuron apoptosis through regulation of Bcl-2 and Bax expression in CA1 region of hippocampus.
