CAJ | 학술논문

目的探讨脑源性神经营养因子(BDNF)基因多态性与内化性精神障碍之间的关联.方法采用多重聚合酶链反应(PCR)和连接酶检测(LDR)的方法,对259例诊断为内化性精神障碍大学生和269名健康大学生BDNF基因上的3个tag SNPs位点进行基因型检测,对结果进行单倍型分析和多位点联合分析.结果各位点在病例组和对照组中的基因型分布均符合Hardy-Weinberg平衡.rs12273539位点在病例和对照组中的基因型和等位基因的频数分布差异均无统计学意义(P值均＞0.05).rs10835210和rs2030324位点在病例和对照组中的基因型和等位基因的频数分布差异均有统计学意义(P值均＜0.05).病例组中rs10835210的A等位基因和rs2030324的C等位基因频率明显高于对照组,携带有rs10835210的A等位基因和rs2030324的C等位基因是内化性精神障碍发生的危险因素(OR=1.877,P＜0.001;OR=1.347,P＜0.05).多位点联合作用分析显示,这3个位点的联合作用与内化性精神障碍相关联(χ~2=23.537,P＜0.001).结论 BDNF基因可能是引起内化性精神障碍发病的易感基因.
목적 탐토뇌원성신경영양인자(BDNF)기인다태성여내화성정신장애지간적관련.방법 채용다중취합매련반응(PCR)화련접매검측(LDR)적방법,대259례진단위내화성정신장애대학생화269명건강대학생BDNF기인상적3개tag SNPs위점진행기인형검측,대결과진행단배형분석화다위점연합분석.결과 각위점재병례조화대조조중적기인형분포균부합Hardy-Weinberg평형.rs12273539위점재병례화대조조중적기인형화등위기인적빈수분포차이균무통계학의의(P치균＞0.05).rs10835210화rs2030324위점재병례화대조조중적기인형화등위기인적빈수분포차이균유통계학의의(P치균＜0.05).병례조중rs10835210적A등위기인화rs2030324적C등위기인빈솔명현고우대조조,휴대유rs10835210적A등위기인화rs2030324적C등위기인시내화성정신장애발생적위험인소(OR=1.877,P＜0.001;OR=1.347,P＜0.05).다위점연합작용분석현시,저3개위점적연합작용여내화성정신장애상관련(χ~2=23.537,P＜0.001).결론 BDNF기인가능시인기내화성정신장애발병적역감기인.
Objective To investigate the genetic association between brain-derived neurotrophic factor(BDNF)gene polymorphism and internalizing disorders,to provide the theoretical basis to explore the etiology of internalizing disorders.Methods PCR-based ligase detection reaction(PCR-LDR)was applied to tag single nucleotide lengh polymorphism(SNPs)of BDNF gene among 259 undergraduates affected by internalizing disorders and 269 healthy undergraduates.Haplotype analysis and multiple locus analysis were conducted to analyze the genotyping data.Results The genotypic frequency of tag SNPs of BDNF gene did not deviate from Hardy-Weinberg equilibrium in both case and control groups.Rsl2273539 was not associated with internalizing disorders(P＞0.05),but rs10835210 and rs2030324 were related to internalizing disorders(P＜0.05).The case group had more A allele of rs10835210 and C allele of rs2030324 when compared to the controls while A allele of rs 10835210 and C allele of rs2030324 seemed to be the risk factors of internalizing disorder(OR=1.877,P＜0.001;OR=1.347,P＜0.05).Results of multiple locus analysis showed that the haplotype composed by the three tag SNPs which was related to internalizing disorders(χ~2=23.537,P＜0.001).Conclusion BDNF gene might serve as the susceptible gene for internalizing disorder.