中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2009年
12期
1277-1280
,共4页
边长玲%龚正达%张丽云%栗冬梅%葛军旗%李四全%李璋鸿%魏丽荣
邊長玲%龔正達%張麗雲%慄鼕梅%葛軍旂%李四全%李璋鴻%魏麗榮
변장령%공정체%장려운%률동매%갈군기%리사전%리장홍%위려영
嗜吞噬细胞无形体%16S rRNA基因%Msp4基因%小型兽类
嗜吞噬細胞無形體%16S rRNA基因%Msp4基因%小型獸類
기탄서세포무형체%16S rRNA기인%Msp4기인%소형수류
Anaplasma phagocytophilum%16S rRNA gene%Msp4 gene%Small mammals
目的 了解中国西南横断山区小型兽类自然感染嗜吞噬细胞无形体的情况.方法 采集位于滇西北横断山区的高黎贡山山脉、香格里拉雪山等山地(海拔1000～4500m)林区的小型兽类脏器标本以低温保存和运输,所获标本在实验室应用聚合酶链反应(PCR)方法对嗜吞噬细胞无形体16S rRNA基因和Msp4基因片段进行扩增测序,并将所测序列与GenBank中注册的基因序列进行相似性比较.结果 共检测小型兽类5目18属35种共436只,从6属11种小型兽类中发现阳性标本32份,总阳性率为7.34%.其中,高黎贡山林区检测小型兽类标本25种301只,阳性26份,阳性率为8.64%(26/301);香格里拉雪山等林区检测小型兽类标本19种135只,阳性6份,阳性率为4.44%(6/135);阳性标本绝大部分发现于小型兽类中的啮齿类动物.序列比较分析表明:不同小型兽类间的16S rRNA基因序列相似性为99%～100%,且与吉林野鼠中检测的无形体相对应片段(GenBank:DQ449948)最相近,相似性达99%～100%.对其Msp4基因核苷酸序列进一步分析发现与GenBank中相应片段相似性为95%～97%,提示中国西南横断山区小型兽类所感染无形体株变异较大.结论 首次证实和发现中国西南横断山区6属11种小型兽类自然感染嗜吞噬细胞无形体,其中啮齿类动物可能是这一地区嗜吞噬细胞无形体的主要宿主.
目的 瞭解中國西南橫斷山區小型獸類自然感染嗜吞噬細胞無形體的情況.方法 採集位于滇西北橫斷山區的高黎貢山山脈、香格裏拉雪山等山地(海拔1000～4500m)林區的小型獸類髒器標本以低溫保存和運輸,所穫標本在實驗室應用聚閤酶鏈反應(PCR)方法對嗜吞噬細胞無形體16S rRNA基因和Msp4基因片段進行擴增測序,併將所測序列與GenBank中註冊的基因序列進行相似性比較.結果 共檢測小型獸類5目18屬35種共436隻,從6屬11種小型獸類中髮現暘性標本32份,總暘性率為7.34%.其中,高黎貢山林區檢測小型獸類標本25種301隻,暘性26份,暘性率為8.64%(26/301);香格裏拉雪山等林區檢測小型獸類標本19種135隻,暘性6份,暘性率為4.44%(6/135);暘性標本絕大部分髮現于小型獸類中的齧齒類動物.序列比較分析錶明:不同小型獸類間的16S rRNA基因序列相似性為99%～100%,且與吉林野鼠中檢測的無形體相對應片段(GenBank:DQ449948)最相近,相似性達99%～100%.對其Msp4基因覈苷痠序列進一步分析髮現與GenBank中相應片段相似性為95%～97%,提示中國西南橫斷山區小型獸類所感染無形體株變異較大.結論 首次證實和髮現中國西南橫斷山區6屬11種小型獸類自然感染嗜吞噬細胞無形體,其中齧齒類動物可能是這一地區嗜吞噬細胞無形體的主要宿主.
목적 료해중국서남횡단산구소형수류자연감염기탄서세포무형체적정황.방법 채집위우전서북횡단산구적고려공산산맥、향격리랍설산등산지(해발1000～4500m)림구적소형수류장기표본이저온보존화운수,소획표본재실험실응용취합매련반응(PCR)방법대기탄서세포무형체16S rRNA기인화Msp4기인편단진행확증측서,병장소측서렬여GenBank중주책적기인서렬진행상사성비교.결과 공검측소형수류5목18속35충공436지,종6속11충소형수류중발현양성표본32빈,총양성솔위7.34%.기중,고려공산림구검측소형수류표본25충301지,양성26빈,양성솔위8.64%(26/301);향격리랍설산등림구검측소형수류표본19충135지,양성6빈,양성솔위4.44%(6/135);양성표본절대부분발현우소형수류중적교치류동물.서렬비교분석표명:불동소형수류간적16S rRNA기인서렬상사성위99%～100%,차여길림야서중검측적무형체상대응편단(GenBank:DQ449948)최상근,상사성체99%～100%.대기Msp4기인핵감산서렬진일보분석발현여GenBank중상응편단상사성위95%～97%,제시중국서남횡단산구소형수류소감염무형체주변이교대.결론 수차증실화발현중국서남횡단산구6속11충소형수류자연감염기탄서세포무형체,기중교치류동물가능시저일지구기탄서세포무형체적주요숙주.
Objective To investigate the prevalence of Anaplasma phagocytophilum in small mammals from the forest area of Hengduan Mountains in southwestern China.Methods Small mammals captured from Gaoligong and Xianggelila mountainous area of Yunnan province were detected by PCR amplification.The sequences of 16S rRNA and Msp4 gene fragments from positive samples were compared with corresponding sequences deposited in GenBank.Results A total number of 436 small animals,which belongs to 5 orders 18 genera 35 species were tested,32(7.34%)were positive in 6 genera 11 species.There were 8.64%(26/301)positive in 25 species at Goligong mountainous areas,and 4.44%(6/135)were positive in 19 species at the Xianggelila mountainous areas.Positive small mammals were most rodents.The nucleotide sequences of A.phagocytophilum 16S rRNA gene amplified from small mammals varied from 99%-100% and were 99%-100% similar with the corresponding segments of A.phagocytophilum from Jilin deposited in GeneBank.The sequences of A.phagocytophilum Msp4 gene showed that there was 95%-97% similarity with the corresponding sequences registered in GenBank.Conclusion A.phagocytophilum was firstly identified in 6 genera 11 species small mammals from a forest area of Hengduan Mountainous areas in southwestern China.Rodents might serve as the primary hosts indicating the potential risk to the domestic animals and human beings in this area.
