CAJ | 학술논문

目的了解2011年广州市登革热的流行情况,分析新分离毒株的E基因分子特征.方法收集2011年广州市登革热的流行病学资料和血清标本.采用荧光定量PCR检测并确定血清型,C6/36细胞进行病毒分离,测定新分离毒株的E基因序列,利用Mega 4.0软件分析.结果 2011年广州市登革热发病高峰在9-11月.在患者血清中检测出登革热病毒1、2、4型,分离出5株登革热1型毒株.在基因型上,4株属于亚洲型,1株属于美洲/非洲型.结论广州市登革病毒与东南亚地区的毒株有较高同源性,且存在登革热暴发的潜在风险,该病毒在广州市可能已出现本地化趋势.
목적 료해2011년엄주시등혁열적류행정황,분석신분리독주적E기인분자특정.방법 수집2011년엄주시등혁열적류행병학자료화혈청표본.채용형광정량PCR검측병학정혈청형,C6/36세포진행병독분리,측정신분리독주적E기인서렬,이용Mega 4.0연건분석.결과 2011년엄주시등혁열발병고봉재9-11월.재환자혈청중검측출등혁열병독1、2、4형,분리출5주등혁열1형독주.재기인형상,4주속우아주형,1주속우미주/비주형.결론 엄주시등혁병독여동남아지구적독주유교고동원성,차존재등혁열폭발적잠재풍험,해병독재엄주시가능이출현본지화추세.
Objective To investigate the epidemiological characteristics of Dengue and the E gene of the new isolated strains.Methods Epidemiological data and serum samples were collected.Serotypes were detected by real-time PCR and virus was isolated in C6/36.E gene of the new isolated strains were sequenced and analyzed by Mega 4.0.Results The cases of Dengue reached at the peak during September and November,with Serotype 1,2 and 4 were involved.Five strains of serotype 1 were isolated,with 4 of them fell into the clad of Asia genotype,and 1 belonged to America/Africa genotype.Conclusion The strains isolated in Guangzhou showed a high identity to the Southeast Asian strains.There seemed high risk of outbreak of Dengue in this area,However,the Dengue virus might have already been localized.