中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2013年
3期
210-213
,共4页
董晓莲%姚晴青%王学才%徐海涛%王晓丽%陈胜玉%唐智峰%郑英杰
董曉蓮%姚晴青%王學纔%徐海濤%王曉麗%陳勝玉%唐智峰%鄭英傑
동효련%요청청%왕학재%서해도%왕효려%진성옥%당지봉%정영걸
隐匿性乙型肝炎病毒感染%显性乙型肝炎病毒感染%进化特征分析%基因型
隱匿性乙型肝炎病毒感染%顯性乙型肝炎病毒感染%進化特徵分析%基因型
은닉성을형간염병독감염%현성을형간염병독감염%진화특정분석%기인형
Occult hepatitis B virus infection%Overt hepatitis B virus Infection%Phylogenetic analysis%Genotype
目的 了解母婴匹配人群隐匿性HBV感染(OBI)及显性和隐匿性病毒株S基因的变异.方法 基础调查时随访135对母婴,填写调查表及采血样,采用ELISA检测HBsAg,Real-time PCR测定病毒载量,PCR扩增HBV S基因并进行进化分析.结果 共采集母血94份,其子女血样101份,OBI感染率分别为10.0％和2.0％,母亲显著高于其子女;显性和隐匿性病毒株病毒载量中位数分别为399.9 IU/ml和247.6 IU/ml,两者差异无统计学意义;OBI病毒株有1株为B基因型、adw血清型,其余7株均为C基因型、adr血清型;显性HBV感染病毒株有15株为B基因型、adw血清型,其余8株为C基因型、adr血清型;OBI病毒株中C基因型比例显著高于显性HBV病毒株.结论 母婴人群存在一定比例的OBI,应重视其预防.
目的 瞭解母嬰匹配人群隱匿性HBV感染(OBI)及顯性和隱匿性病毒株S基因的變異.方法 基礎調查時隨訪135對母嬰,填寫調查錶及採血樣,採用ELISA檢測HBsAg,Real-time PCR測定病毒載量,PCR擴增HBV S基因併進行進化分析.結果 共採集母血94份,其子女血樣101份,OBI感染率分彆為10.0％和2.0％,母親顯著高于其子女;顯性和隱匿性病毒株病毒載量中位數分彆為399.9 IU/ml和247.6 IU/ml,兩者差異無統計學意義;OBI病毒株有1株為B基因型、adw血清型,其餘7株均為C基因型、adr血清型;顯性HBV感染病毒株有15株為B基因型、adw血清型,其餘8株為C基因型、adr血清型;OBI病毒株中C基因型比例顯著高于顯性HBV病毒株.結論 母嬰人群存在一定比例的OBI,應重視其預防.
목적 료해모영필배인군은닉성HBV감염(OBI)급현성화은닉성병독주S기인적변이.방법 기출조사시수방135대모영,전사조사표급채혈양,채용ELISA검측HBsAg,Real-time PCR측정병독재량,PCR확증HBV S기인병진행진화분석.결과 공채집모혈94빈,기자녀혈양101빈,OBI감염솔분별위10.0％화2.0％,모친현저고우기자녀;현성화은닉성병독주병독재량중위수분별위399.9 IU/ml화247.6 IU/ml,량자차이무통계학의의;OBI병독주유1주위B기인형、adw혈청형,기여7주균위C기인형、adr혈청형;현성HBV감염병독주유15주위B기인형、adw혈청형,기여8주위C기인형、adr혈청형;OBI병독주중C기인형비례현저고우현성HBV병독주.결론 모영인군존재일정비례적OBI,응중시기예방.
Objective Prevalence of occult hepatitis B virus (HBV) infection (OBI) was investigated in a paired mother-teenager population and HBV S gene variation including overt and occult HBV,was determined.Methods A follow-up study based on an initial survey of 135 mother-teenager pairs was carried out through collection of questionnaires and blood samples HBsAg were detected by ELISA method,viral load by PCR amplification and HBV S gene by phylogenetic analysis.Results 102 pairs of subjects were followed-up.Blood samples from 94 mothers and 101children were collected.OBI prevalence in mothers was 10.0％ (6/60),significantly higher than 2.0％(2/101) in teenagers.Medians of viral load were 399.9 IU/ml and 247.6 IU/ml in overt and occult HBV strains,but without significant difference.1 occult HBV strain belonged to genotype B with serotype adw while the other 7 were genotype C with serotype adr.15 of the overt HBV strains belonged to genotype B with serotype adw and the other 8 were genotype C with serotype adr.Proportions of genotype-C strains were significantly higher in occult HBV strains than in overt HBV strains.Conclusion OBI was seen in teenage-mother population.
