CAJ | 학술논문

目的探讨北京地区急性胃肠炎患者诺如病毒分子流行病学特征.方法采用RT-PCR扩增核酸和测序分型方法.结果共收集腹泻标本685份,检测诺如病毒阻性66份,阳性率为9.6％.检出率在患者性别、年龄、住院和门诊间的差异均无统计学意义;诺如病毒胃肠炎患者时间分布也无明显季节性.66份阳性标本采用ORF2区测序分型,其中43份可分型.GⅠ组占22.7％,包括GⅠ.3型2份、G Ⅰ.4型1份、GⅠ.5型4份、GⅠ.7型3份;GⅡ组占77.3％,其中以GⅡ.4型为主(60.6％),其他包括GⅡ.2型1份、GⅡ.3型1份,GⅡ.5型1份、GⅡ.6型2份、GⅡ.7型1份、GⅡ.12型7份.6例出现GⅠ组和GⅡ组混合感染,其中可分型的有G Ⅰ.3/GⅡ.7、GⅠ.5/GⅡ.5、G Ⅰ.4/GⅡ.4.结论北京地区急性胃肠炎患者诺如病毒呈现基因多样性,且以GⅡ组为主,其中GⅡ.4/2006a和2006b是主要的流行变种,存在GⅠ和GⅡ组混合感染现象.
목적 탐토북경지구급성위장염환자낙여병독분자류행병학특정.방법 채용RT-PCR확증핵산화측서분형방법.결과 공수집복사표본685빈,검측낙여병독조성66빈,양성솔위9.6％.검출솔재환자성별、년령、주원화문진간적차이균무통계학의의;낙여병독위장염환자시간분포야무명현계절성.66빈양성표본채용ORF2구측서분형,기중43빈가분형.GⅠ조점22.7％,포괄GⅠ.3형2빈、G Ⅰ.4형1빈、GⅠ.5형4빈、GⅠ.7형3빈;GⅡ조점77.3％,기중이GⅡ.4형위주(60.6％),기타포괄GⅡ.2형1빈、GⅡ.3형1빈,GⅡ.5형1빈、GⅡ.6형2빈、GⅡ.7형1빈、GⅡ.12형7빈.6례출현GⅠ조화GⅡ조혼합감염,기중가분형적유G Ⅰ.3/GⅡ.7、GⅠ.5/GⅡ.5、G Ⅰ.4/GⅡ.4.결론 북경지구급성위장염환자낙여병독정현기인다양성,차이GⅡ조위주,기중GⅡ.4/2006a화2006b시주요적류행변충,존재GⅠ화GⅡ조혼합감염현상.
Objective To study the prevalence,genotypes and molecular characteristics of norovirus (NoV) in acute gastroenteritis.Methods RT-PCR was used to determine the molecular epidemiology of NoV.Results Out of 685 samples,66 positive specimens were identified and the prevalence was 9.6％ (66/685),9.9％ in males and 9.4％ in females,respectively,with no significant difference.The prevalence rates showed no differences between age groups or between inpatients and outpatients.NoV gastroenteritis did not present any seasonal distribution.43 out of the 66 specimens were classified,with 10 (22.7％) belonged to G Ⅰ including 2 G Ⅰ.3,1 G Ⅰ.4,4 G Ⅰ.5 and 3 G Ⅰ.7.Other 33 (77.3％) belonged to G Ⅱ genogroup,including G Ⅱ.4 accounted for 60.6％ (20/33) and followed by 7 G Ⅱ.12,2 G Ⅱ.6,1 G Ⅱ.2,1 G ⅡⅡ.3,1 G Ⅱ.5.Six specimens mixed with G Ⅰ and G Ⅱ and 3 specimens were classified as G Ⅰ.3/G Ⅱ.7,G Ⅰ.5/G Ⅱ.5 and G Ⅰ.4/G Ⅱ.4.Conclusion The main symptoms of acute gastroenteritis were abdominal pain,nausea,vomit and fever.There were many genotypes identified in our study and the main genotypes were G Ⅱ.4/2006a and 2006b.G Ⅰ and G Ⅱ could be coinfected with each other.