中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2013年
6期
560-565
,共6页
赵小元%张美仙%程红%闫银坤%吴丽君%沈玥%米杰
趙小元%張美仙%程紅%閆銀坤%吳麗君%瀋玥%米傑
조소원%장미선%정홍%염은곤%오려군%침모%미걸
肥胖%基因%单核苷酸多态性%随访%儿童期
肥胖%基因%單覈苷痠多態性%隨訪%兒童期
비반%기인%단핵감산다태성%수방%인동기
Obesity%Gene%Single nucleotide polymorphism%Follow up%Childhood
目的 前瞻性分析肥胖相关基因多态性对儿童期肥胖发生及持续状态的影响.方法 基于北京市儿童青少年代谢综合征(BCAMS)研究,对部分仍在校者进行6年后随访,测量身高和体重,以随访到的780名儿童青少年为研究对象.采用盐析法从外周血白细胞中提取DNA.通过查阅文献和专家研讨,确定6个基因的7个多态性位点(SNP),其中FTO rs9939609分型使用ABI-5700实时定量PCR仪,其他6个SNP位点(FTO rs6499640、FAIM2 rs7138803、NPCIrs1805081、MC4R rs 17782313、BDNF rs6265、GNPDA2 rs10938397)的分型使用ABI PrismsTM-7900实时荧光定量PCR仪.采用中国肥胖问题工作组推荐的BMI分类标准判定超重和肥胖.计量资料组间比较采用独立样本t检验,分类资料组间比较采用x2检验.采用多因素logistic回归分析肥胖相关基因多态性对儿童期肥胖发生风险和持续状态的影响.结果 随访6年肥胖累积发病率为8.5％,基线肥胖的儿童青少年有65.1％随访6年后仍持续肥胖.FTO rs9939609基因型TT、TA和AA组肥胖发病率依次升高(趋势检验x2=8.030,P＜0.05)；控制随访时点年龄、性别和城乡居住地后,携带FTO rs9939609A等位基因者相对于无该等位基因者发生肥胖的OR值及95％CI为2.42(1.31 ～ 4.47).与基线非肥胖且不携带rs9939609A等位基因组比较,携带rs9939609A等位基因组、基线肥胖组、基线肥胖同时携带rs9939609 A危险等位基因组随访时点罹患肥胖的风险分别为OR=2.07 (95％ CI:0.95 ～ 4.47)、OR=22.01 (95％CI:13.27 ～ 36.49)和OR=45.95 (95％CI:24.00～87.94).其他SNP与肥胖的关联无统计学意义(P＞0.05).综合分析多个SNP的共同效应发现,遗传危险得分每增加1个单位,肥胖的发病风险增加16.42倍(95％CI:3.59～75.10,P＜0.001).结论 FTO rs9939609对儿童期新发肥胖及肥胖的持续状态都具有一定影响.多个肥胖相关基因的综合效应对肥胖发病的影响不可忽视.
目的 前瞻性分析肥胖相關基因多態性對兒童期肥胖髮生及持續狀態的影響.方法 基于北京市兒童青少年代謝綜閤徵(BCAMS)研究,對部分仍在校者進行6年後隨訪,測量身高和體重,以隨訪到的780名兒童青少年為研究對象.採用鹽析法從外週血白細胞中提取DNA.通過查閱文獻和專傢研討,確定6箇基因的7箇多態性位點(SNP),其中FTO rs9939609分型使用ABI-5700實時定量PCR儀,其他6箇SNP位點(FTO rs6499640、FAIM2 rs7138803、NPCIrs1805081、MC4R rs 17782313、BDNF rs6265、GNPDA2 rs10938397)的分型使用ABI PrismsTM-7900實時熒光定量PCR儀.採用中國肥胖問題工作組推薦的BMI分類標準判定超重和肥胖.計量資料組間比較採用獨立樣本t檢驗,分類資料組間比較採用x2檢驗.採用多因素logistic迴歸分析肥胖相關基因多態性對兒童期肥胖髮生風險和持續狀態的影響.結果 隨訪6年肥胖纍積髮病率為8.5％,基線肥胖的兒童青少年有65.1％隨訪6年後仍持續肥胖.FTO rs9939609基因型TT、TA和AA組肥胖髮病率依次升高(趨勢檢驗x2=8.030,P＜0.05)；控製隨訪時點年齡、性彆和城鄉居住地後,攜帶FTO rs9939609A等位基因者相對于無該等位基因者髮生肥胖的OR值及95％CI為2.42(1.31 ～ 4.47).與基線非肥胖且不攜帶rs9939609A等位基因組比較,攜帶rs9939609A等位基因組、基線肥胖組、基線肥胖同時攜帶rs9939609 A危險等位基因組隨訪時點罹患肥胖的風險分彆為OR=2.07 (95％ CI:0.95 ～ 4.47)、OR=22.01 (95％CI:13.27 ～ 36.49)和OR=45.95 (95％CI:24.00～87.94).其他SNP與肥胖的關聯無統計學意義(P＞0.05).綜閤分析多箇SNP的共同效應髮現,遺傳危險得分每增加1箇單位,肥胖的髮病風險增加16.42倍(95％CI:3.59～75.10,P＜0.001).結論 FTO rs9939609對兒童期新髮肥胖及肥胖的持續狀態都具有一定影響.多箇肥胖相關基因的綜閤效應對肥胖髮病的影響不可忽視.
목적 전첨성분석비반상관기인다태성대인동기비반발생급지속상태적영향.방법 기우북경시인동청소년대사종합정(BCAMS)연구,대부분잉재교자진행6년후수방,측량신고화체중,이수방도적780명인동청소년위연구대상.채용염석법종외주혈백세포중제취DNA.통과사열문헌화전가연토,학정6개기인적7개다태성위점(SNP),기중FTO rs9939609분형사용ABI-5700실시정량PCR의,기타6개SNP위점(FTO rs6499640、FAIM2 rs7138803、NPCIrs1805081、MC4R rs 17782313、BDNF rs6265、GNPDA2 rs10938397)적분형사용ABI PrismsTM-7900실시형광정량PCR의.채용중국비반문제공작조추천적BMI분류표준판정초중화비반.계량자료조간비교채용독립양본t검험,분류자료조간비교채용x2검험.채용다인소logistic회귀분석비반상관기인다태성대인동기비반발생풍험화지속상태적영향.결과 수방6년비반루적발병솔위8.5％,기선비반적인동청소년유65.1％수방6년후잉지속비반.FTO rs9939609기인형TT、TA화AA조비반발병솔의차승고(추세검험x2=8.030,P＜0.05)；공제수방시점년령、성별화성향거주지후,휴대FTO rs9939609A등위기인자상대우무해등위기인자발생비반적OR치급95％CI위2.42(1.31 ～ 4.47).여기선비비반차불휴대rs9939609A등위기인조비교,휴대rs9939609A등위기인조、기선비반조、기선비반동시휴대rs9939609 A위험등위기인조수방시점리환비반적풍험분별위OR=2.07 (95％ CI:0.95 ～ 4.47)、OR=22.01 (95％CI:13.27 ～ 36.49)화OR=45.95 (95％CI:24.00～87.94).기타SNP여비반적관련무통계학의의(P＞0.05).종합분석다개SNP적공동효응발현,유전위험득분매증가1개단위,비반적발병풍험증가16.42배(95％CI:3.59～75.10,P＜0.001).결론 FTO rs9939609대인동기신발비반급비반적지속상태도구유일정영향.다개비반상관기인적종합효응대비반발병적영향불가홀시.
Objective To examine the impact of single nucleotide polymorphisms (SNPs) in obesity-related genes on the incidence and durative of obesity in childhood and adolescence.Methods Based on the Beijing Child and Adolescent Metabolic Syndrome (BCAMS) Study,780 school children aged 6 to 16 years were followed-up in 2010,and assessed for body size parameters.Venipuncture blood samples were collected after a 12-hour overnight fast.Genomic DNA was isolated from peripheral blood white cells under the salt fractionation method.SNPs were genotyped by ABI 5700 real time PCR (FTO rs9939609) and TaqMan Allelic Discrimination Assays with the GeneAmp 7900 Sequence Detection System (Applied Biosystems,Foster City,CA,USA) (FTO rs6499640,FAIM2 rs7138803,NPC1 rs1805081,MC4R rs17782313,BDNF rs6265,GNPDA2 rs10938397).Both overweight and obesity were diagnosed by the Chinese age-and sex-specific body mass index (BMI)cutoffs.Two independent sample t-test,Chi-square test and multiple logistic regression analysis were performed.Results During the 6 years follow-up period,the incidence of obesity in the total sample 8.5％,and 65.1％ individuals had persisted their obese status.The genotypes of the SNPs except BDNF rs6265 were in Hardy-Weinberg equilibrium in each group (P＞0.05).The incidence rates of obesity increased with FTO rs9939609 TT,TA and AA genotypes (x2 for trend=8.030,P＜0.05).In the non-obese sub-cohort,after adjusted for sex,age at the initial time of follow up and residential area,when compared with children carrying FTO rs9939609 T-allele,a significantly relative risk of obesity was observed for children carrying the rs9939609 A-allele (OR=2.42,95％CI:1.31-4.47,P=0.005).In the obese sub-cohort,FTO rs9939609 A-allele was significantly associated with durative of obesity (OR=1.72,95％CI:1.07-2.77,P=0.026).However,no statistical significant associations were seen between other SNPs (FTO rs6499640,FAIM2 rs7138803,NPCI rs1805081,MC4R rs17782313,GNPDA2 rs10938397) and the incidence or durative of obesity (all P＞0.05).The genetic risk scorewas associated with the risk of occurrence of obesity (OR=16.42,95％ CI:3.59-75.10,P＜0.001)after adjusted for residential area,sex,age at the initial time of follow up and baseline BMI.Conclusion We confirmed the association of FTO rs9939609 with incidence and durative of obesityin children.Early intervention was recommended on the high risk individuals who carrying more riskalleles in obesity-related genes.
