CAJ | 학술논문

目的评估沙门菌常规检测方法分段控制技术在网络实验室建设中的基础性作用.方法建立经过关键点技术控制评价的沙门菌检测方法,评估上海市参加世界卫生组织-全球沙门菌监测项目(WHO-GSS)、中美新发和再发传染病项目(GFN)网络实验室的实施,培训云南省玉溪市疾病预防控制中心腹泻标本沙门菌常规检测能力,收集2006-2012年省级GSS-GFN监测点年度沙门菌监测阳性率.结果基于分段控制技术设计的沙门菌分离、鉴定和种属鉴定、血清分群方法,能同时满足网络实验室对伤寒、非伤寒沙门菌检测敏感性需求；上海市网络实验室建设从2006年的5个公共卫生实验室和8个临床实验室发展到2011年的9和22个,伤寒、非伤寒沙门菌临床分离菌株从2006年的196株增加到2011年1442株；2012年云南省玉溪市临床腹泻病例沙门菌阳性率为2.4％；除上海外还有3个省级监测点将亚硒酸盐磺绿增菌液(SBG)作为沙门菌选择性增菌液,以上海市沙门菌监测基线最稳定.结论常规沙门菌检测分段优化的方法是构建区域网络实验室的基础,由此可上升为具有精确表型鉴定和分子分型能力的国家网络实验室.
목적 평고사문균상규검측방법분단공제기술재망락실험실건설중적기출성작용.방법 건립경과관건점기술공제평개적사문균검측방법,평고상해시삼가세계위생조직-전구사문균감측항목(WHO-GSS)、중미신발화재발전염병항목(GFN)망락실험실적실시,배훈운남성옥계시질병예방공제중심복사표본사문균상규검측능력,수집2006-2012년성급GSS-GFN감측점년도사문균감측양성솔.결과 기우분단공제기술설계적사문균분리、감정화충속감정、혈청분군방법,능동시만족망락실험실대상한、비상한사문균검측민감성수구；상해시망락실험실건설종2006년적5개공공위생실험실화8개림상실험실발전도2011년적9화22개,상한、비상한사문균림상분리균주종2006년적196주증가도2011년1442주；2012년운남성옥계시림상복사병례사문균양성솔위2.4％；제상해외환유3개성급감측점장아서산염광록증균액(SBG)작위사문균선택성증균액,이상해시사문균감측기선최은정.결론 상규사문균검측분단우화적방법시구건구역망락실험실적기출,유차가상승위구유정학표형감정화분자분형능력적국가망락실험실.
Objective To evaluated the fundamental role of stage control technology (SCT)on the detectability for Salmonella networking laboratories.Methods Appropriate Salmonella detection methods after key point control being evaluated,were establishment and optimized.Our training and evaluation networking laboratories participated in the World Health Organization-Global Salmonella Surveillance Project (WHO-GSS) and China-U.S.Collaborative Program on Emerging and Re-emerging infectious diseases Project (GFN) in Shanghai.Staff members from the Yunnan Yuxi city Center for Disease Control and Prevention were trained on Salmonella isolation from diarrhea specimens.Data on annual Salmonella positive rates was collected from the provincial-level monitoring sites to be part of the GSS and GFN projects from 2006 to 2012.Results The methodology was designed based on the conventional detection procedure of Salmonella which involved the processes as enrichment,isolation,species identification and sero-typing.These methods were simultaneously used to satisfy the sensitivity requirements on non-typhoid Salmonella detection for networking laboratories.Public Health Laboratories in Shanghai had developed from 5 in 2006 to 9 in 2011,and Clinical laboratories from 8 to 22.Number of clinical isolates,including typhoid and non-typhoid Salmonella increased from 196 in 2006 to 1442 in 2011.The positive rate of Salmonella isolated from the clinical diarrhea cases was 2.4％ in Yuxi county,in 2012.At present,three other provincial monitoring sites were using the SBG technique as selectivity enrichment broth for Salmonella isolation,with Shanghai having the most stable positive baseline.Conclusion The method of SCT was proved the premise of the network laboratory construction.Based on this,the improvement of precise phenotypic identification and molecular typing capabilities could reach the level equivalent to the national networking laboratory.