中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2013年
12期
1213-1215
,共3页
马红霞%潘静静%康锴%谢志强%茹维萍%陈豪敏%黄学勇%许汴利
馬紅霞%潘靜靜%康鍇%謝誌彊%茹維萍%陳豪敏%黃學勇%許汴利
마홍하%반정정%강개%사지강%여유평%진호민%황학용%허변리
柯萨奇病毒B5型%脑炎%系统进化分析
柯薩奇病毒B5型%腦炎%繫統進化分析
가살기병독B5형%뇌염%계통진화분석
Coxsackievirus B5%Encephalitis%Phylogenetic analysis
目的 分析河南省柯萨奇病毒B5(CVB5)分离株全基因序列,了解其遗传特性.方法 采集2例脑炎患者临床标本,分离病毒,提取病毒阳性分离物RNA,进行RT-PCR扩增,产物直接测序.利用DNAStar 5.01和Mega 5.05软件进行全基因序列拼接及亲缘进化分析.结果 获得2株CVB5分离株(03001N和17Y)全基因组序列,核苷酸长度分别为7408 bp和7404 bp,两者核苷酸同源性为97％,同2010年河南CVB5分离株同源性最高,分别为98％和99％.5'-UTR分别为747 bp和743 bp;3’-UTR区均为103bp;病毒基因组编码区全长同为6558 bp,编码含2185个氨基酸残基的多聚蛋白,氨基酸同源性为99％.VP1区系统发生树分析显示,2株CVB5分离株进化关系较近,同近年来国内其他分离株成一簇,而2009年的河南省CVB5分离株同山东省CVB5分离株成一簇.结论 河南省内存在不同CVB5株传播链的流行,此次分离株为近几年内的主要流行株.
目的 分析河南省柯薩奇病毒B5(CVB5)分離株全基因序列,瞭解其遺傳特性.方法 採集2例腦炎患者臨床標本,分離病毒,提取病毒暘性分離物RNA,進行RT-PCR擴增,產物直接測序.利用DNAStar 5.01和Mega 5.05軟件進行全基因序列拼接及親緣進化分析.結果 穫得2株CVB5分離株(03001N和17Y)全基因組序列,覈苷痠長度分彆為7408 bp和7404 bp,兩者覈苷痠同源性為97％,同2010年河南CVB5分離株同源性最高,分彆為98％和99％.5'-UTR分彆為747 bp和743 bp;3’-UTR區均為103bp;病毒基因組編碼區全長同為6558 bp,編碼含2185箇氨基痠殘基的多聚蛋白,氨基痠同源性為99％.VP1區繫統髮生樹分析顯示,2株CVB5分離株進化關繫較近,同近年來國內其他分離株成一簇,而2009年的河南省CVB5分離株同山東省CVB5分離株成一簇.結論 河南省內存在不同CVB5株傳播鏈的流行,此次分離株為近幾年內的主要流行株.
목적 분석하남성가살기병독B5(CVB5)분리주전기인서렬,료해기유전특성.방법 채집2례뇌염환자림상표본,분리병독,제취병독양성분리물RNA,진행RT-PCR확증,산물직접측서.이용DNAStar 5.01화Mega 5.05연건진행전기인서렬병접급친연진화분석.결과 획득2주CVB5분리주(03001N화17Y)전기인조서렬,핵감산장도분별위7408 bp화7404 bp,량자핵감산동원성위97％,동2010년하남CVB5분리주동원성최고,분별위98％화99％.5'-UTR분별위747 bp화743 bp;3’-UTR구균위103bp;병독기인조편마구전장동위6558 bp,편마함2185개안기산잔기적다취단백,안기산동원성위99％.VP1구계통발생수분석현시,2주CVB5분리주진화관계교근,동근년래국내기타분리주성일족,이2009년적하남성CVB5분리주동산동성CVB5분리주성일족.결론 하남성내존재불동CVB5주전파련적류행,차차분리주위근궤년내적주요류행주.
Objective To analyze the complete genome sequences of two coxsackievirus B5 (CVB5) isolated in Henan province,2011.Methods Specimens were collected from viral encephalitis patients and followed by viral isolation on them.RNA were extracted from positive isolates and the amplified products were sequenced.The full-length genomes of them were acquired by assembling the fragments,using DNAStar 5.01 software while phylogenetic analysis were performed with Mega 5.05 and other software.Results The genomes RNA of 03001N and 17Y showed 7408 bp and 7404 bp long,and the 5'-and 3'-untranslated regions were 747 bp,743 bp and 103 bp,103 bp,respectively.BLAST analysis of these two isolates,based on the complete genome,showed 97％ identity,with both of them having the highest similarity (98％,99％) to the CVB5 strain isolated from Henan in 2010 rather than other CVB5 strains.Coding regions of both isolates were 6558 bp,code for a polyprotein of 2185 amino acids (aa) and both of them showed 99％ amino acid identity.Phylogenetic tree in VP1 region showed that the two isolates belonged to the same clade with other strains isolated from all over the country in the past years,except for some CVB5 strains isolated from Henan and Shandong province in 2009 that formed the other cluster.Conclusion It seemed that more than one group of CVB5 were circulating in Henan province and these two isolates appeared the main epidemic strains circulating in the past years.
