CAJ | 학술논문

目的明确苏州地区腹泻婴幼儿博卡病毒感染情况、病毒主要基因型别及其流行病学特征.方法收集2010-2011年苏州市儿童医院疑似病毒性腹泻患儿粪便标本832例(份),采用荧光定量PCR方法检测粪便中博卡病毒DNA,并对部分阳性标本测序明确病毒基因型.结果 832份粪便标本检出博卡病毒阳性51份,阳性率为6.1％.7-9月为博卡病毒感染高峰;96％的患儿为＜2岁,其中7～12月龄为高发年龄段;男性患儿多于女性;36例合并其他腹泻病毒感染.对35份博卡病毒阳性标本进行测序,其中28份为HBoV-1型,5份为HBoV-2型,2份型别未确定,未发现HBoV-3型及HBoV-4型.结论苏州地区婴幼儿腹泻标本中博卡病毒以HBoV-l型为主,同时存在HBoV-2型感染.
목적 명학소주지구복사영유인박잡병독감염정황、병독주요기인형별급기류행병학특정.방법 수집2010-2011년소주시인동의원의사병독성복사환인분편표본832례(빈),채용형광정량PCR방법검측분편중박잡병독DNA,병대부분양성표본측서명학병독기인형.결과 832빈분편표본검출박잡병독양성51빈,양성솔위6.1％.7-9월위박잡병독감염고봉;96％적환인위＜2세,기중7～12월령위고발년령단;남성환인다우녀성;36례합병기타복사병독감염.대35빈박잡병독양성표본진행측서,기중28빈위HBoV-1형,5빈위HBoV-2형,2빈형별미학정,미발현HBoV-3형급HBoV-4형.결론 소주지구영유인복사표본중박잡병독이HBoV-l형위주,동시존재HBoV-2형감염.
Objective To investigate the status of human bocavirus and to indentify its epidemio[ogical characteristics as well as genotype distribution in patients with infantile viral diarrhea in Suzhou,Jiangsu province.Methods 832 fecal specimens from patients with infantile virus diarrhea cases were collected from Suzhou Children's Hospital in 2010-2011.Human bocavirus were detected by Real-Time RT-PCR,and genotype were determined by sequence analysis.Results Among all the fecal specimens,51 (6.1％) cases were positive for human bocavirus.The peak season of rotavirus infection was between July and September.Of all the episodes on rotavirus diarrhea,96％ occurred before 2 years of age,with peaks in children with 7-12 months of age.Data from Nucleotide Sequence analysis showed that among 28 samples that carrying HBoV-1,5 strains belonged to HBoV-2,HBoV type 3 but type 4 were absent.Conclusion Human bocavirus were detected from fecal specimens of infantile virus diarrhea in Suzhou,with genotype HBoV-1 as the major strain.HBoV-2 genotype was also found.