CAJ | 학술논문

目的分析2012-2013年冬季中国地区诺如病毒新流行株GⅡ.4/Sydney 2012的衣壳蛋白区核苷酸与氨基酸变异特点.方法对已获得的22份2012-2013年冬季北京地区GⅡ.4/Sydney 2012株进行全衣壳蛋白区基因扩增.从GenBank检索中国其他地区的GⅡ.4/Sydney 2012株衣壳蛋白区核苷酸序列.对获得的GⅡ.4/Sydney 2012株的衣壳蛋白区核苷酸及氨基酸序列与往年GⅡ.4流行株进行系谱分析.结果获得中国7个地区(北京、上海、江苏、湖州、荆州、香港和台湾)的GⅡ.4/Sydney 2012株资料共38份(至少包含完整VP1核苷酸序列).GⅡ.4/Sydney 2012株之间VP1核苷酸差异为0.1％～3.3％,氨基酸差异为0～3.1％.系谱分析发现GⅡ.4/Sydney2012株与Apeldoom 2008和New Orleans 2009起自共同的分支.中国和悉尼GⅡ.4/Sydney 2012代表株的A、D、E抗原表位氨基酸序列组合有两种:TSRN-GTT-SNT和TSRN-STT-SNT.结论 GⅡ.4/Sydney 2012株已在中国多个地区出现,各地区病毒株同源性较高.GⅡ.4/Sydney 2012株的抗原表位氨基酸组合发生明显改变.
목적 분석2012-2013년동계중국지구낙여병독신류행주GⅡ.4/Sydney 2012적의각단백구핵감산여안기산변이특점.방법 대이획득적22빈2012-2013년동계북경지구GⅡ.4/Sydney 2012주진행전의각단백구기인확증.종GenBank검색중국기타지구적GⅡ.4/Sydney 2012주의각단백구핵감산서렬.대획득적GⅡ.4/Sydney 2012주적의각단백구핵감산급안기산서렬여왕년GⅡ.4류행주진행계보분석.결과 획득중국7개지구(북경、상해、강소、호주、형주、향항화태만)적GⅡ.4/Sydney 2012주자료공38빈(지소포함완정VP1핵감산서렬).GⅡ.4/Sydney 2012주지간VP1핵감산차이위0.1％～3.3％,안기산차이위0～3.1％.계보분석발현GⅡ.4/Sydney2012주여Apeldoom 2008화New Orleans 2009기자공동적분지.중국화실니GⅡ.4/Sydney 2012대표주적A、D、E항원표위안기산서렬조합유량충:TSRN-GTT-SNT화TSRN-STT-SNT.결론 GⅡ.4/Sydney 2012주이재중국다개지구출현,각지구병독주동원성교고.GⅡ.4/Sydney 2012주적항원표위안기산조합발생명현개변.
Objective To study the nucleotide and amino acid sequences of norovirus G Ⅱ.4/Sydney 2012 variants,in China.Methods Twenty-two stool specimens,confirmed as G Ⅱ.4/Sydney 2012-positive were collected from Beijing in the winter of 2012-2013.RT-PCR was performed to target the complete capsid gene.G Ⅱ.4/Sydney 2012 strains from other regions in China were searched and obtained from the GenBank.Nucleotide and amino acid sequences of G Ⅱ.4/Sydney 2012 strains were analyzed,using the CLUSTAL X (Version 1.83)and followed by phylogenetic analysis using Mega version 5.1.Results The complete major capsid nucleotide sequences of thirty-eight G Ⅱ.4/Sydney 2012 strains from seven regions in China were obtained.The VP1 nucleotide and amino acid sequences diversity were 0.1％-3.3％ and 0-3.1％,respectively.Result from phylogenetic analysis demonstrated that the G Ⅱ.4/Sydney 2012 variant shared a common ancestor with both the dominant norovirus G Ⅱ.4 variants Apeldoom 2008 and the New Orleans 2009.G Ⅱ.4/Sydney 2012 variants appeared to have had two A/D/E site combinations at the amino acid level,TSRN-GTT-SNT and TSRN-STT-SNT.Conclusion G Ⅱ.4/Sydney 2012 variant had been circulating in many regions in China.There seemed a high nucleotide and amino acid identity among G Ⅱ.4/Sydney 2012 strains collected from China.G Ⅱ.4/Sydney 2012 variants showed different A/D/E site combination from other pandemic G Ⅱ.4 variants.