中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2014年
3期
299-302
,共4页
彭晓旻%刘爽%杨鹏%李静%张代涛%崔淑娟%吴双胜%刘医萌%王全意
彭曉旻%劉爽%楊鵬%李靜%張代濤%崔淑娟%吳雙勝%劉醫萌%王全意
팽효민%류상%양붕%리정%장대도%최숙연%오쌍성%류의맹%왕전의
A组链球菌%超抗原%emm基因分型%儿童
A組鏈毬菌%超抗原%emm基因分型%兒童
A조련구균%초항원%emm기인분형%인동
Group A streptoccccus%Superantigen%emm typing%Children
目的 分析2011年北京市儿童来源A组链球菌(GAS)临床分离株13种超抗原基因的携带情况及其与病例特征和emm分型间的关系.方法 2011年5-7月在北京市36家医院收集儿童来源GAS临床分离株635株.采用实时荧光PCR方法检测菌株超抗原基因speA、speB、speC、speF、speG、speH、speI、speJ、speK、speL、speM、smeZ、ssa的携带情况,PCR法扩增M蛋白N末端基因片段,并对产物测序确定GAS的emm型别.结果 13种超抗原基因的携带率分别为speA (22.4％)、speB (100.0％)、speC (99.4％)、speF (99.7％)、speG (99.7％)、speH (76.4％)、speI(76.2％) 、speJ(21.7％)、speK(0.6％) 、speL(1.1％) 、speM(2.2％) 、smeZ(99.7％)、ssa(98.0％),共观察到26种超抗原谱.超抗原speA、speH、speI、speJ在emm1和emm12型GAS间分布的差异有统计学意义(P＜0.05).致咽部感染菌株超抗原speK和speL的检出率高于致猩红热菌株(P＜0.05)；而对ssa的携带率低于致猩红热菌株(P＜0.05).结论 2011年北京市儿童来源GAS对超抗原基因speB 、speF、smeZ 、speG 、speC和ssa的携带率较高,speM、speL和speK的携带率较低.GAS的emm分型与超抗原基因间存在密切联系.未观察到超抗原基因speA和speC在致猩红热和致咽部感染菌株间的分布差异.
目的 分析2011年北京市兒童來源A組鏈毬菌(GAS)臨床分離株13種超抗原基因的攜帶情況及其與病例特徵和emm分型間的關繫.方法 2011年5-7月在北京市36傢醫院收集兒童來源GAS臨床分離株635株.採用實時熒光PCR方法檢測菌株超抗原基因speA、speB、speC、speF、speG、speH、speI、speJ、speK、speL、speM、smeZ、ssa的攜帶情況,PCR法擴增M蛋白N末耑基因片段,併對產物測序確定GAS的emm型彆.結果 13種超抗原基因的攜帶率分彆為speA (22.4％)、speB (100.0％)、speC (99.4％)、speF (99.7％)、speG (99.7％)、speH (76.4％)、speI(76.2％) 、speJ(21.7％)、speK(0.6％) 、speL(1.1％) 、speM(2.2％) 、smeZ(99.7％)、ssa(98.0％),共觀察到26種超抗原譜.超抗原speA、speH、speI、speJ在emm1和emm12型GAS間分佈的差異有統計學意義(P＜0.05).緻嚥部感染菌株超抗原speK和speL的檢齣率高于緻猩紅熱菌株(P＜0.05)；而對ssa的攜帶率低于緻猩紅熱菌株(P＜0.05).結論 2011年北京市兒童來源GAS對超抗原基因speB 、speF、smeZ 、speG 、speC和ssa的攜帶率較高,speM、speL和speK的攜帶率較低.GAS的emm分型與超抗原基因間存在密切聯繫.未觀察到超抗原基因speA和speC在緻猩紅熱和緻嚥部感染菌株間的分佈差異.
목적 분석2011년북경시인동래원A조련구균(GAS)림상분리주13충초항원기인적휴대정황급기여병례특정화emm분형간적관계.방법 2011년5-7월재북경시36가의원수집인동래원GAS림상분리주635주.채용실시형광PCR방법검측균주초항원기인speA、speB、speC、speF、speG、speH、speI、speJ、speK、speL、speM、smeZ、ssa적휴대정황,PCR법확증M단백N말단기인편단,병대산물측서학정GAS적emm형별.결과 13충초항원기인적휴대솔분별위speA (22.4％)、speB (100.0％)、speC (99.4％)、speF (99.7％)、speG (99.7％)、speH (76.4％)、speI(76.2％) 、speJ(21.7％)、speK(0.6％) 、speL(1.1％) 、speM(2.2％) 、smeZ(99.7％)、ssa(98.0％),공관찰도26충초항원보.초항원speA、speH、speI、speJ재emm1화emm12형GAS간분포적차이유통계학의의(P＜0.05).치인부감염균주초항원speK화speL적검출솔고우치성홍열균주(P＜0.05)；이대ssa적휴대솔저우치성홍열균주(P＜0.05).결론 2011년북경시인동래원GAS대초항원기인speB 、speF、smeZ 、speG 、speC화ssa적휴대솔교고,speM、speL화speK적휴대솔교저.GAS적emm분형여초항원기인간존재밀절련계.미관찰도초항원기인speA화speC재치성홍열화치인부감염균주간적분포차이.
Objective To analyze the prevalence of super-antigens (SAgs) of group A streptococcus (GAS) isolated from Beijing pediatric patients in 2011,and to explore the relationship between emm types,characteristics of patients and SAgs.Methods A total of 635 isolates of GAS were collected from children in 36 hospitals in Beijing from May to July,2011.Thirteen currently known SAg genes were tested by real-time PCR,and emm gene was performed by PCR and sequencing of N-terminal gene fragrnents of M protein.Results Prevalence rates of 13 SAg genes spe A,speB,speC,speF,speG,speH,spe I,speJ,speK,speL,speM,smeZ,ssa were 22.4 ％,100.0 ％,99.4％,99.7％,99.7％,76.4％,76.2％,21.7％,0.6％,1.1％,2.2％,99.7％ and 98.0％,respectively.A total of 26 SAgs profiles were observed according to the SAgs inclusion.There were significant differences in frequencies of speA,speH,spel and speJ between emm 1 and emm 12 strains (P ＜ 0.05).In isolates from patients with pharyngeal infection,the prevalence rates for speK and speL were higher while the frequency for ssa was lower than that from scarlet fever cases (P＜0.05).Conclusion The frequencies of speB,speF,smeZ,speG,,speC,and ssa were high among strains isolated while speM,speL and speK were relatively low from children in Beijing,2011.SAg genes appeared to be associated with the emm types.In this study,differences of frequency for speA and speC from strains colleted from patients with scarlet fever and pharyngeal infection had not been found.
