中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2014年
3期
307-311
,共5页
刘建生%邵聪文%赵卫中%张云昆%吉玛%朱艳菊%马忠飞%马绍辉
劉建生%邵聰文%趙衛中%張雲昆%吉瑪%硃豔菊%馬忠飛%馬紹輝
류건생%소총문%조위중%장운곤%길마%주염국%마충비%마소휘
柯萨奇B组5型病毒%全基因组%序列分析%进化树图
柯薩奇B組5型病毒%全基因組%序列分析%進化樹圖
가살기B조5형병독%전기인조%서렬분석%진화수도
Coxsackievirus B5%Complete genome%Sequence analysis%Phylogenetic analysis
目的 分析柯萨奇病毒B5(CVB5)云南分离株A210/KM/09全基因序列及其遗传特性.方法 设计针对CVB5引物,提取病毒RNA,RT-PCR扩增和产物直接测序获得序列,利用Mega 4.1、RDP3和SimPlot 3.5.1软件分析全基因序列.结果 CVB5 A210/KM/09全基因组核苷酸序列长度为7 372 bp,编码含2 185个氨基酸残基的多聚蛋白；A210/KM/09全基因组核苷酸及所推导的氨基酸与CVB5/CC10/10同源性最高,其同源性分别为92.5％和97.3％；而与其他CVB5毒株如17Y、19CSF、20CSF、1954/85/US、2000/CSF/KOR、03001N、CoxB5/Henan/2010、CVB5/SD/09和Faulkner核苷酸和氨基酸同源性分别为80.1％ ～ 92.5％和95.0％～ 97.3％;A210/KM/09与其他CVB5毒株的各区段核苷酸和氨基酸同源性分别为75.3％ ～ 96.3％和85.3％～100.0％,其中与VP3、3D区段核苷酸的同源性最低.基于CVB5全长VP1基因序列进行种系进化分析,可将CVB5分为A、B、C和D4个基因型,其中C基因型可再分为C1～C4基因亚型,D基因型可再分为D1～D4基因亚型.中国CVB5流行株主要聚集在C4基因亚型,国外流行株主要聚集在D1、C2亚型.结论 A210/KM/09分离株为C4基因型.
目的 分析柯薩奇病毒B5(CVB5)雲南分離株A210/KM/09全基因序列及其遺傳特性.方法 設計針對CVB5引物,提取病毒RNA,RT-PCR擴增和產物直接測序穫得序列,利用Mega 4.1、RDP3和SimPlot 3.5.1軟件分析全基因序列.結果 CVB5 A210/KM/09全基因組覈苷痠序列長度為7 372 bp,編碼含2 185箇氨基痠殘基的多聚蛋白；A210/KM/09全基因組覈苷痠及所推導的氨基痠與CVB5/CC10/10同源性最高,其同源性分彆為92.5％和97.3％；而與其他CVB5毒株如17Y、19CSF、20CSF、1954/85/US、2000/CSF/KOR、03001N、CoxB5/Henan/2010、CVB5/SD/09和Faulkner覈苷痠和氨基痠同源性分彆為80.1％ ～ 92.5％和95.0％～ 97.3％;A210/KM/09與其他CVB5毒株的各區段覈苷痠和氨基痠同源性分彆為75.3％ ～ 96.3％和85.3％～100.0％,其中與VP3、3D區段覈苷痠的同源性最低.基于CVB5全長VP1基因序列進行種繫進化分析,可將CVB5分為A、B、C和D4箇基因型,其中C基因型可再分為C1～C4基因亞型,D基因型可再分為D1～D4基因亞型.中國CVB5流行株主要聚集在C4基因亞型,國外流行株主要聚集在D1、C2亞型.結論 A210/KM/09分離株為C4基因型.
목적 분석가살기병독B5(CVB5)운남분리주A210/KM/09전기인서렬급기유전특성.방법 설계침대CVB5인물,제취병독RNA,RT-PCR확증화산물직접측서획득서렬,이용Mega 4.1、RDP3화SimPlot 3.5.1연건분석전기인서렬.결과 CVB5 A210/KM/09전기인조핵감산서렬장도위7 372 bp,편마함2 185개안기산잔기적다취단백；A210/KM/09전기인조핵감산급소추도적안기산여CVB5/CC10/10동원성최고,기동원성분별위92.5％화97.3％；이여기타CVB5독주여17Y、19CSF、20CSF、1954/85/US、2000/CSF/KOR、03001N、CoxB5/Henan/2010、CVB5/SD/09화Faulkner핵감산화안기산동원성분별위80.1％ ～ 92.5％화95.0％～ 97.3％;A210/KM/09여기타CVB5독주적각구단핵감산화안기산동원성분별위75.3％ ～ 96.3％화85.3％～100.0％,기중여VP3、3D구단핵감산적동원성최저.기우CVB5전장VP1기인서렬진행충계진화분석,가장CVB5분위A、B、C화D4개기인형,기중C기인형가재분위C1～C4기인아형,D기인형가재분위D1～D4기인아형.중국CVB5류행주주요취집재C4기인아형,국외류행주주요취집재D1、C2아형.결론 A210/KM/09분리주위C4기인형.
Objective To characterize the complete genome sequence of coxsackievirus B5 (CVB5) A210/KM/09 strain which was isolated from Yunnan,China,2009.Methods Eight overlapping clones covering the whole viral genome (excluding the poly-A tail) were obtained by RT-PCR and sequenced,with their nucleotide and amino acid sequences compared with other known CVB5 strains.Results The genome of the CVB5 A210/KM/09 strain had 7 372 nucleotides in length,and containing a 742-nt non-translated region (NTR) at the 5' end and a 98-nt NTR at the 3' end.The entire open reading frame contained 6 555 nt,encoding a 2 185-aa polyprotein.In the coding region,there appeared no nucleotide deletion or insertion,but some changes of amino acid seemed unique.Based on the complete genome sequence alignments,CVB5 isolate A210/KM/09 strain showed the highest nucleotide (92.5％) and amino acid (97.3％) identities to the CVB5/CC10/10.It also shared nucleotide(80.1％-92.5％) and amino acid (95.0％-97.3％) homology with other CVB5 strains:17Y,19CSF,20CSF,1954/85/US,2000/CSF/KOR,03001N,CoxBS/Henan/2010,VB5/SD/09 and Faulkner.Blast between genome fragments,A210/KM/09 showed similarity on nucleotide (80.1％-92.5％) and amino acid (95.0％-97.3％) identities with other CVB5 strains.The phylogenetic tree,constructed on the complete VP1 regions,indicated that CVB5 could be divided into genotype A,B,C and D.while Genotype C and D could be further divided into C1-C4 and D1-D4 subgenotypes.Conclusion A210/KM/09 and other CVB5 predominant strains isolated in China belonged to CVB5 subgenotype C4.
