CAJ | 학술논문

目的了解西安市MSM感染HIV-1毒株亚型分布.方法采集2010--2012年西安市确证的经男男同性传播的HIV感染者外周静脉血5 ml,抗凝后分离血浆,提取总RNA,利用巢式反转录PCR扩增HIV-1 gag和enw基因,利用Mega 5.2软件与国际参考序列进行拼接、比对、计算基因离散率和构建系统进化树;同时进行流行病学问卷调查,行为学调查重点包括性行为特征、吸毒史、献血史等.结果 168份样本中,165例gag与env序列分型结果一致,分别为79例(47.0％)CRF01_AE、74例(44.0％)CRF07_BC、12例(7.1％)B亚型.3例gag与env序列分型结果不一致,分别为2例(1.2％)CRF01_AE/A1、1例(0.6％)CRF07_BC/CRF0 1_AE.结论西安市MSM感染HIV-1毒株亚型主要为CRF01 _AE、CRF07_BC.
목적 료해서안시MSM감염HIV-1독주아형분포.방법 채집2010--2012년서안시학증적경남남동성전파적HIV감염자외주정맥혈5 ml,항응후분리혈장,제취총RNA,이용소식반전록PCR확증HIV-1 gag화enw기인,이용Mega 5.2연건여국제삼고서렬진행병접、비대、계산기인리산솔화구건계통진화수;동시진행류행병학문권조사,행위학조사중점포괄성행위특정、흡독사、헌혈사등.결과 168빈양본중,165례gag여env서렬분형결과일치,분별위79례(47.0％)CRF01_AE、74례(44.0％)CRF07_BC、12례(7.1％)B아형.3례gag여env서렬분형결과불일치,분별위2례(1.2％)CRF01_AE/A1、1례(0.6％)CRF07_BC/CRF0 1_AE.결론 서안시MSM감염HIV-1독주아형주요위CRF01 _AE、CRF07_BC.
Objective To investigate the subtype distribution of HIV-1 infection among men having sex with men (MSM) in Xi' an city.Methods 5 ml anti-coagulating blood samples were collected from MSM who had been reported during 2010-2012 in Xi'an.Both gag and env genes were amplified by nested RT-PCR from the extracted RNA and then sequenced.The acquired sequences were compared with international subtype references,and then the genetic distances were calculated and phylogenetic trees were constructed by Mega 5.2.Epidemiological information including sexual behavior characteristics,history of drug use,blood donation,etc.were gathered.Results 168 samples were successfully amplified and sequenced.Results from Phylogenic analysis showed that the gag and env sequences of 165 samples shared the same subtypes,of which 79 (47.0％) were CRF01_AE,74 (44.0％) were CRF07_BC and 12 (7.1％) belonged to subtype B.There were 3 samples with gag and env sequences classified into different subtypes,of which 2 (1.2％) were CRF01_AE/A1,and the other 1 was CRF07_BC/CRF01_AE.Conclusion At least three HIV-1 subtypes including CRF01_AE,CRF07_BC were identified among MSM population in Xi'an city.