CAJ | 학술논문

目的探讨慢性砷暴露人群DNA氧化损伤与血脂异常的关联性.方法采用分层整群抽样方法,随机抽取某饮水型高砷暴露地区407名慢性砷暴露者作为调查对象,检测其血脂水平,并据此分为血脂正常组、HDL-C比值异常组、高TC血症组和HDL-C比值异常合并高TC血症组.采集调查对象晨尿,并使用ELISA试剂盒测定其DNA氧化损伤的关键性标志物8-羟基脱氧鸟苷/肌酐(8-OHdG/Cr)水平.采用广义线性回归模型调整相关混杂因素后,分析慢性砷暴露人群DNA氧化损伤与血脂异常的关联性.结果 4组人群8-OHdG/Cr水平M(Q1～Q3)分别为51.43(36.86～68.57)、55.73(39.90～ 79.94)、58.08(44.94～69.91)和65.28(49.29～ 92.95)ng/mg,各组间差异有统计学意义(P=0.006).广义线性回归模型进一步表明,在校正血压、文化程度、暴露年限、BMI、FPG、性别、主动吸烟、被动吸烟、吸烟指数和饮酒等潜在的混杂因素后,HDL-C比值异常合并高TC血症组8-OHdG/Cr水平与血脂水平呈现出显著正相关(P=0.018),且8-OHdG/Cr水平与其血脂异常程度间存在明显的线性趋势(P=0.016).结论慢性砷暴露人群8-OHdG/Cr水平与其血脂状态间存在显著的关联性,血脂异常程度越严重,其DNA氧化损伤越明显.
목적 탐토만성신폭로인군DNA양화손상여혈지이상적관련성.방법 채용분층정군추양방법,수궤추취모음수형고신폭로지구407명만성신폭로자작위조사대상,검측기혈지수평,병거차분위혈지정상조、HDL-C비치이상조、고TC혈증조화HDL-C비치이상합병고TC혈증조.채집조사대상신뇨,병사용ELISA시제합측정기DNA양화손상적관건성표지물8-간기탈양조감/기항(8-OHdG/Cr)수평.채용엄의선성회귀모형조정상관혼잡인소후,분석만성신폭로인군DNA양화손상여혈지이상적관련성.결과 4조인군8-OHdG/Cr수평M(Q1～Q3)분별위51.43(36.86～68.57)、55.73(39.90～ 79.94)、58.08(44.94～69.91)화65.28(49.29～ 92.95)ng/mg,각조간차이유통계학의의(P=0.006).엄의선성회귀모형진일보표명,재교정혈압、문화정도、폭로년한、BMI、FPG、성별、주동흡연、피동흡연、흡연지수화음주등잠재적혼잡인소후,HDL-C비치이상합병고TC혈증조8-OHdG/Cr수평여혈지수평정현출현저정상관(P=0.018),차8-OHdG/Cr수평여기혈지이상정도간존재명현적선성추세(P=0.016).결론 만성신폭로인군8-OHdG/Cr수평여기혈지상태간존재현저적관련성,혈지이상정도월엄중,기DNA양화손상월명현.
Objective To explore the association between dyslipidemia and the level of 8-OHdG/Cr in urine among a population exposed to chronic arsenic.Methods Four hundred and seven subjects were randomly selected in an arsenic-affected area in Inner Mongolia.After blood biochemical examination,all the subjects were divided into 4 groups based on the results of total cholesterol (TC),triglycerides (TG),high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C).The groups consisted of hypercholesterolemia,HDL-C ratio anomaly,combined hypercholesterolemia and HDL-C ratio anomaly,as well as a normal lipid group.Urine samples were collected and 8-OHdG/Cr was measured using the ELISA method.A generalized linear mixed model was used to analyze the association between dyslipidemia and 8-OHdG/Cr.Results The levels of 8-OHdG/Cr as 55.73 (39.90-79.94) ng/mg,58.08 (44.94-69.91) ng/mg,65.28 (49.29-92.95) ng/mg and 51.43 (36.86-68.57)ng/mgin the HDL-C ratio anomaly,hypercholesterolemia,combined hypercholesterolemia and HDL-C ratio anomaly groups and the control group,respectively,which showed significant differences on the levels of 8-OHdG/Cr in the four groups (P=0.006).From the linear regression analysis results showed that the 8-OHdG/Cr level incombined hypercholesterolemia and HDL-C ratio anomaly group was higher (4.25 ± 0.55 ng/mg) than in the control group (3.96 ± 0.55 ng/mg) (P=0.018).After adjusting for important covariates,there was a linear trend between the levels of 8-OHdG/Cr and dyslipidemia (P=0.016).Conclusion Data from our study showed a linear relation between hypercholesterolemia,HDL-C ratio anomaly and the 8-OHdG/Cr level,suggesting that dyslipidemia was associated with oxidative DNA damage among those arsenic-affected people.