CAJ | 학술논문

目的分析广东省2009-2013年霍乱病例及环境来源O1/O139群霍乱弧菌病原学特征.方法选取2009-2013年广东省霍乱病例来源、环境(水体和海水产品)来源的O1/O139群霍乱弧菌.采用血清分型、抗菌药物敏感性试验、毒力基因PCR检测和脉冲场凝胶电泳(PFGE)分子分型方法,研究不同来源的霍乱弧菌血清型、抗菌药物敏感性、毒力基因携带以及分子分型方面的异同.结果 2009-2013年广东省共分离得到各类来源O1/O139群霍乱弧菌190株(病例16株,外环境174株).病例来源菌株分为O1群稻叶型(3株)、小川型(7株)和O139群(6株)3种菌型;其中10株ctxA基因阳性,2株小川型菌株携带不完整CTXΦ噬菌体;5株菌对11种抗菌药物完全敏感,3株对4种抗菌药物表现出耐受.外环境来源菌株中53株稻叶型,22株小川型和2株O139群菌株携带不完整CTXΦ噬菌体;2株O139菌株检出ctxA基因阳性;25株对≥4种抗菌药物耐受,其中有2株同时对11种抗菌药物中的7种耐受,以水产品中的稻叶型菌株为主(13株).PFGE分子分型结果显示,菌株经Not Ⅰ酶切后的PFGE型别表现出明显的多样性.稻叶型和O139群病例菌株的带型聚集在同一个聚类中,小川型病例菌株带型分散在不同的聚类中,病例来源菌株与环境来源菌株的带型差别较大.结论广东省O1/O139群霍乱弧菌毒力基因和遗传特征复杂多样,菌株多重耐药形势严峻,需要加强菌株型别变异及耐药监测.
목적 분석광동성2009-2013년곽란병례급배경래원O1/O139군곽란호균병원학특정.방법 선취2009-2013년광동성곽란병례래원、배경(수체화해수산품)래원적O1/O139군곽란호균.채용혈청분형、항균약물민감성시험、독력기인PCR검측화맥충장응효전영(PFGE)분자분형방법,연구불동래원적곽란호균혈청형、항균약물민감성、독력기인휴대이급분자분형방면적이동.결과 2009-2013년광동성공분리득도각류래원O1/O139군곽란호균190주(병례16주,외배경174주).병례래원균주분위O1군도협형(3주)、소천형(7주)화O139군(6주)3충균형;기중10주ctxA기인양성,2주소천형균주휴대불완정CTXΦ서균체;5주균대11충항균약물완전민감,3주대4충항균약물표현출내수.외배경래원균주중53주도협형,22주소천형화2주O139군균주휴대불완정CTXΦ서균체;2주O139균주검출ctxA기인양성;25주대≥4충항균약물내수,기중유2주동시대11충항균약물중적7충내수,이수산품중적도협형균주위주(13주).PFGE분자분형결과현시,균주경Not Ⅰ매절후적PFGE형별표현출명현적다양성.도협형화O139군병례균주적대형취집재동일개취류중,소천형병례균주대형분산재불동적취류중,병례래원균주여배경래원균주적대형차별교대.결론 광동성O1/O139군곽란호균독력기인화유전특정복잡다양,균주다중내약형세엄준,수요가강균주형별변이급내약감측.
Objective To analyze the etiologic characteristics of O1/O139 Vibrio cholerae in Guangdong province in 2009-2013.Methods Isolates from cholera cases and from the environment surveillance points were investigated by serological typing,antibiotic susceptibility testings,toxic genes detection and molecular typing to analyze the similarities and differences of the identified species.Results Totally,190 isolations of O1/O139 V.cholerae were obtained from cholera cases (16 strains) and environmental samples (174 strains) in Guangdong province in 2009-2013.The sero-types would include Inaba (3 isolates),Ogawa (7 isolates) and O139 (6 isolates) in all the isolates from the cholera cases.Ten strains from the ctxA positive cases were detected by PCR.Two Ogawa strains carried incomplete CTXΦ phage.Results from the antibiotic susceptibility test indicated that 5 strains were absolutely sensitive to 11 antibiotic discs in vitro,while another 3 strains were simultaneously resistant to 4 antibiotic discs.Except for 2 stains,all the O139 strains from the environment were ctxA negative,detected by PCR.Incomplete CTXΦ phage was found in the Inaba (53 isolates),Ogawa (22 isolates) and O139 (2 isolates),respectively.Results from the antibiotic susceptibility test exhibited that 25 strains were resistant simultaneously to 4 and/or more antibiotic discs,especially the Inaba strains from the seafoods (13 isolates).2 Inaba strains from seafood were simultaneously resistant to 7 antibiotic discs.Results from PFGE molecular typing indicated that the PFGE types digested by Not Ⅰ expressed significant diversity.Inaba and O139 strains from cases were gathered in the same clusters,while the Ogawa strains from cases scattered in different clusters but no significant correlation smong these strains were found.Our results suggested that a distant genetic relationship might exist between these two different sources strains.Conclusion Complex and diverse as the virulence genes and genetic characteristics and with the grim situation of multi-drug resistant strains,all seemed important to strengthen the surveillance programs on the variation of strain types and antibiotics resistance ofO1/O139 V.cholerae in Guangdong province.