中华流行病学杂志
中華流行病學雜誌
중화류행병학잡지
CHINESE JOURNAL OF EPIDEMIOLOGY
2014年
7期
837-841
,共5页
王晓光%雷永良%陶晓燕%李浩%申辛欣%于鹏程%尹翠萍%孟胜利%王欣莹
王曉光%雷永良%陶曉燕%李浩%申辛訢%于鵬程%尹翠萍%孟勝利%王訢瑩
왕효광%뢰영량%도효연%리호%신신흔%우붕정%윤취평%맹성리%왕흔형
狂犬病病毒%宿主动物%基因%序列分析
狂犬病病毒%宿主動物%基因%序列分析
광견병병독%숙주동물%기인%서렬분석
Rabies virus%Host%Gene%Sequencing analyses
目的 测定浙江省不同宿主(人、鼬獾、犬)来源的狂犬病病毒街毒株N基因序列,分析病毒遗传变异特征及其与流行的关系.方法 采用直接免疫荧光试验和反转录聚合酶链式反应检测狂犬病病毒阳性标本N基因核苷酸序列,利用生物信息学软件分析基因序列和编码蛋白.结果 共获得浙江省2个人源、5个鼬獾源和11个犬源狂犬病病毒街毒株N基因核苷酸序列,18个核苷酸和氨基酸序列同源性在89.7％～ 100.0％和98.4％～ 100.0％,N蛋白一级结构上绝大部分为稳定区域,编码基因的核苷酸变异多为无义突变,系统发育分析显示18个街毒株均属于传统的基因1型.结论 浙江省不同宿主来源狂犬病病毒的流行具有地域性特征,同类宿主动物病毒株或来自同一县域/相邻县域的毒株在地理位置上最为近缘,但人源株病毒更具有复杂性.浙江省狂犬病病毒街毒株的流行具有通过犬向鼬獾和人传播,并在犬、鼬獾中跨区域循环传播的特点.
目的 測定浙江省不同宿主(人、鼬獾、犬)來源的狂犬病病毒街毒株N基因序列,分析病毒遺傳變異特徵及其與流行的關繫.方法 採用直接免疫熒光試驗和反轉錄聚閤酶鏈式反應檢測狂犬病病毒暘性標本N基因覈苷痠序列,利用生物信息學軟件分析基因序列和編碼蛋白.結果 共穫得浙江省2箇人源、5箇鼬獾源和11箇犬源狂犬病病毒街毒株N基因覈苷痠序列,18箇覈苷痠和氨基痠序列同源性在89.7％～ 100.0％和98.4％～ 100.0％,N蛋白一級結構上絕大部分為穩定區域,編碼基因的覈苷痠變異多為無義突變,繫統髮育分析顯示18箇街毒株均屬于傳統的基因1型.結論 浙江省不同宿主來源狂犬病病毒的流行具有地域性特徵,同類宿主動物病毒株或來自同一縣域/相鄰縣域的毒株在地理位置上最為近緣,但人源株病毒更具有複雜性.浙江省狂犬病病毒街毒株的流行具有通過犬嚮鼬獾和人傳播,併在犬、鼬獾中跨區域循環傳播的特點.
목적 측정절강성불동숙주(인、유환、견)래원적광견병병독가독주N기인서렬,분석병독유전변이특정급기여류행적관계.방법 채용직접면역형광시험화반전록취합매련식반응검측광견병병독양성표본N기인핵감산서렬,이용생물신식학연건분석기인서렬화편마단백.결과 공획득절강성2개인원、5개유환원화11개견원광견병병독가독주N기인핵감산서렬,18개핵감산화안기산서렬동원성재89.7％～ 100.0％화98.4％～ 100.0％,N단백일급결구상절대부분위은정구역,편마기인적핵감산변이다위무의돌변,계통발육분석현시18개가독주균속우전통적기인1형.결론 절강성불동숙주래원광견병병독적류행구유지역성특정,동류숙주동물병독주혹래자동일현역/상린현역적독주재지리위치상최위근연,단인원주병독경구유복잡성.절강성광견병병독가독주적류행구유통과견향유환화인전파,병재견、유환중과구역순배전파적특점.
Objective To elucidate the characteristics of genetic variability and its relationship with prevalence,through sequencing and analysis of N gene among street rabies virus isolated from different hosts (homo sapiens,ferret badger,dog) in Zhejiang province.Methods Samples were screened and confirmed by direct fluorescence assay and reverse transcript PCR.Sequences were analyzed using bio-information software.Results Eighteen street rabies virus strains were identified,including 2 from homo sapiens,5 from ferret badger,and 11 from dog.Similarities of N gene and N protein were calculated to be 89.7％-100.0％ and 98.4％-100.0％ respectively.Mutations occurred in N gene were almost non-sense mutations.In addition,Data from phylogenetic analysis showed that all these strains could be classified into traditional genotype 1.Conclusion The prevalence of rabies viruses among different hosts in Zhejiang province had certain regional properties.Rabies viruses isolated from the same kind of host or from the same/adjacent county/ counties had the closest relationship.However,the characteristics of rabies virus prevalent in homo sapiens were somewhat complicated.In summary,the transmission of street rabies virus in Zhejiang province was from dogs to ferret badgers and homo sapiens,and the virus could circulate and cross-regional transmit among dogs and ferret badgers.
