CAJ | 학술논문

目的调查2012年云南省艾滋病高流行区德宏州未经抗病毒治疗的HIV感染者的基因型分布和耐药株传播水平.方法根据HIV耐药警戒线调查实施方案,对2012年1-8月德宏州符合要求的60份年龄在16 ～ 25岁新发现的HIV-1感染者的血浆样本进行HIV-1耐药基因型检测和耐药株传播水平分析.结果 52份样本完成基因型及耐药鉴定,其中中国籍占59.6％,缅甸籍占40.4％.通过进化分析对pol区进行分型,38.5％的样本为独特型重组(URF),其他依次为C亚型(34.6％)、CRF01_AE(21.2％)、CRF08_BC(3.8％)和B亚型(1.9％).在2个序列中各检测到1个针对非核苷类反转录酶抑制剂(NNRTI)类药物的耐药突变位点,按照耐药警戒线的统计方法估算耐药株流行率为5％～15％,属于中度水平.结论德宏州新感染人群中存在复杂的HIV-1基因型,HIV-1耐药株已处于中度流行水平.
목적 조사2012년운남성애자병고류행구덕굉주미경항병독치료적HIV감염자적기인형분포화내약주전파수평.방법 근거HIV내약경계선조사실시방안,대2012년1-8월덕굉주부합요구적60빈년령재16 ～ 25세신발현적HIV-1감염자적혈장양본진행HIV-1내약기인형검측화내약주전파수평분석.결과 52빈양본완성기인형급내약감정,기중중국적점59.6％,면전적점40.4％.통과진화분석대pol구진행분형,38.5％적양본위독특형중조(URF),기타의차위C아형(34.6％)、CRF01_AE(21.2％)、CRF08_BC(3.8％)화B아형(1.9％).재2개서렬중각검측도1개침대비핵감류반전록매억제제(NNRTI)류약물적내약돌변위점,안조내약경계선적통계방법고산내약주류행솔위5％～15％,속우중도수평.결론 덕굉주신감염인군중존재복잡적HIV-1기인형,HIV-1내약주이처우중도류행수평.
Objective To study the HIV-1 genotypes and transmitted drug resistance (TDR) in Dehong prefecture of Yunnan province in 2012.Methods Referring to the guidelines for HIV drug resistance threshold survey (HIVDR-TS),60 plasma samples of recently reported HIV-infected individuals between 16 and 25 years old were collected in Dehong prefecture from January to August 2012.Genotyping of partial pol gene was performed by using reverse transcriptional PCR.HIV-1 genotype and the prevalent levels of HIV-1 drug resistance transmission were analyzed.Results 52 plasma samples were successfully sequenced and analyzed.Among them,59.6％ were Chinese,and the rest (40.4％) were Burmese.Based on pol sequences,identified HIV genotypes would include unique recombinant forms (URFs,38.5％),subtype C (34.6％),CRF01_AE (21.2％),CRF08_BC (3.8％),and subtype B (1.9％).One drug resistant mutation to non-nucleoside reverse transcriptase inhibitors (NNRTIs) was detected in respective two sequences.Based on the statistical method of HIVDR-TS,the prevalence of transmitted HIV-1 drug resistance was adjusted as a moderate level (5％-15％).Conclusion Diverse HIV-1 genotypes were found in this study,and the current HIV-1 drug resistant strains transmission was catalogued as moderate prevalence level in Dehong.