中华泌尿外科杂志
中華泌尿外科雜誌
중화비뇨외과잡지
CHINESE JOURNAL OF UROLOGY
2013年
11期
823-826
,共4页
闫兵%任雅丽%何群%席志军
閆兵%任雅麗%何群%席誌軍
염병%임아려%하군%석지군
自噬%自噬相关蛋白p-mTOR%自噬相关蛋白Beclin 1%自噬相关蛋白LC3%肾肿瘤
自噬%自噬相關蛋白p-mTOR%自噬相關蛋白Beclin 1%自噬相關蛋白LC3%腎腫瘤
자서%자서상관단백p-mTOR%자서상관단백Beclin 1%자서상관단백LC3%신종류
Autophagy%Autophagy related protein p-mTOR%Autophagy related protein Beclin 1%Autophagy related protein LC3%Kidney neoplasms
目的 检测自噬表达水平在正常组织和肾透明细胞癌组织中的差异. 方法 2011年1-4月采用免疫组化方法检测69例肾透明细胞癌患者的正常组织和肿瘤组织中自噬相关蛋白(p-mTOR、P62、LC3、Beclin 1、ULK1、ATG5、ATG7)表达的差异,并对其表达差异及各蛋白间表达量变化的相关性进行统计学分析.2011年8月采用透射电镜对4例肾癌患者的正常组织和肿瘤组织进行细胞超微结构的分析. 结果 与正常组织相比,p-mTOR(P=0.004)、P62(P=0.000)和ULK1(P=0.000)在肿瘤组织中的表达上调,而Beclin 1(P=0.000)、LC3(P=0.000)和ATG7(P=0.000)的表达下调,差异均有统计学意义.ATG5表达量无明显变化(P=0.349).形态分析结果显示肿瘤细胞内的自噬体数量少于正常组织.p-mTOR与P62(P=0.026)、LC3(P=0.027)和ATG7(P=0.049)的表达水平变化之间具有相关性,而与Beclin 1不相关. 结论 自噬的表达水平在肾透明细胞癌中下调,其下调受mTOR等多种自噬相关蛋白的协同影响.
目的 檢測自噬錶達水平在正常組織和腎透明細胞癌組織中的差異. 方法 2011年1-4月採用免疫組化方法檢測69例腎透明細胞癌患者的正常組織和腫瘤組織中自噬相關蛋白(p-mTOR、P62、LC3、Beclin 1、ULK1、ATG5、ATG7)錶達的差異,併對其錶達差異及各蛋白間錶達量變化的相關性進行統計學分析.2011年8月採用透射電鏡對4例腎癌患者的正常組織和腫瘤組織進行細胞超微結構的分析. 結果 與正常組織相比,p-mTOR(P=0.004)、P62(P=0.000)和ULK1(P=0.000)在腫瘤組織中的錶達上調,而Beclin 1(P=0.000)、LC3(P=0.000)和ATG7(P=0.000)的錶達下調,差異均有統計學意義.ATG5錶達量無明顯變化(P=0.349).形態分析結果顯示腫瘤細胞內的自噬體數量少于正常組織.p-mTOR與P62(P=0.026)、LC3(P=0.027)和ATG7(P=0.049)的錶達水平變化之間具有相關性,而與Beclin 1不相關. 結論 自噬的錶達水平在腎透明細胞癌中下調,其下調受mTOR等多種自噬相關蛋白的協同影響.
목적 검측자서표체수평재정상조직화신투명세포암조직중적차이. 방법 2011년1-4월채용면역조화방법검측69례신투명세포암환자적정상조직화종류조직중자서상관단백(p-mTOR、P62、LC3、Beclin 1、ULK1、ATG5、ATG7)표체적차이,병대기표체차이급각단백간표체량변화적상관성진행통계학분석.2011년8월채용투사전경대4례신암환자적정상조직화종류조직진행세포초미결구적분석. 결과 여정상조직상비,p-mTOR(P=0.004)、P62(P=0.000)화ULK1(P=0.000)재종류조직중적표체상조,이Beclin 1(P=0.000)、LC3(P=0.000)화ATG7(P=0.000)적표체하조,차이균유통계학의의.ATG5표체량무명현변화(P=0.349).형태분석결과현시종류세포내적자서체수량소우정상조직.p-mTOR여P62(P=0.026)、LC3(P=0.027)화ATG7(P=0.049)적표체수평변화지간구유상관성,이여Beclin 1불상관. 결론 자서적표체수평재신투명세포암중하조,기하조수mTOR등다충자서상관단백적협동영향.
Objective To investigate the autophagy capacity in clear cell renal cell carcinoma tissue compared with adjacent normal tissue.Methods Sixty-nine pairs of samples from human clear cell renal cell carcinoma tissues and relatively healthy renal tissues adjacent to the tumor were collected during surgical resection.The expressions of proteins that were participating in the regulation and execution of autophagy were detected by immunohistochemisty.Electron-microscopy was also carried out for the morphometrical analysis.Results The protein expression of p-mTOR (P =0.004),P62 (P =0.000) and ULK1 (P =0.000) were up-regulated in the carcinoma tissue,while the expression of Beclin1 (P=0.000),LC3 (P =0.000) and ATG7 (P =0.000) were down-regulated,and the expression of ATG5 (P =0.349) had no signif-icant difference compared with adjacent normal tissue.Morphometrical analysis showed that the basal autophagic activity (measured by the presence of autophagy vacuole compartment) was remarkably down-regulated in carcinoma tissue,compared with adjacent normal tissue.The expression level of mTOR was correlated with P62,LC3 and ATG7,but results showed no correlation between mTOR and Beclin 1.Conclusion Our studies show a relatively reduced autophagy capacity in clear cell renal cell carcinoma,which is regulated by multiple autophagy-related proteins such as mTOR,Beclin 1 and LC3.
