中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2012年
8期
933-935
,共3页
褪黑激素%免疫,细胞%妇科外科手术
褪黑激素%免疫,細胞%婦科外科手術
퇴흑격소%면역,세포%부과외과수술
Melatonin%Immunity,cellular%Gynecologic surgical procedures
目的 评价人工建立褪黑素节律对妇科手术病人术后细胞免疫功能的影响.方法 择期行妇科手术病人18例,年龄25 ～ 50岁,体重45 ～ 80 kg,ASA分级Ⅰ或Ⅱ级,采用随机数字表法,将其随机分为3组(n=6):正常对照组(C组)、安慰剂对照组(P组)和褪黑素组(M组)、分别于术前1d、手术当天和术后1 d21:00熄灯前10 min时,M组口服褪黑素6 mg,P组口服安慰剂.术中采用连续硬膜外麻醉,术后采用病人自控硬膜外镇痛,维持VAS评分＜5分.分别于术前1d(基础状态)、术毕及术后1d时采集外周静脉血样,采用流式细胞术测定CD4+、CD8+、CD3+细胞数,并计算CD4+与CD8+细胞数的比值.结果 C组、P组和M组间CD4+、CD8+、CD3+细胞数及CD4+与CD8+细胞数的比值差异无统计学意义(P＞0.05).结论 人工建立褪黑素节律对妇科手术病入术后细胞免疫功能无明显影响.
目的 評價人工建立褪黑素節律對婦科手術病人術後細胞免疫功能的影響.方法 擇期行婦科手術病人18例,年齡25 ～ 50歲,體重45 ～ 80 kg,ASA分級Ⅰ或Ⅱ級,採用隨機數字錶法,將其隨機分為3組(n=6):正常對照組(C組)、安慰劑對照組(P組)和褪黑素組(M組)、分彆于術前1d、手術噹天和術後1 d21:00熄燈前10 min時,M組口服褪黑素6 mg,P組口服安慰劑.術中採用連續硬膜外痳醉,術後採用病人自控硬膜外鎮痛,維持VAS評分＜5分.分彆于術前1d(基礎狀態)、術畢及術後1d時採集外週靜脈血樣,採用流式細胞術測定CD4+、CD8+、CD3+細胞數,併計算CD4+與CD8+細胞數的比值.結果 C組、P組和M組間CD4+、CD8+、CD3+細胞數及CD4+與CD8+細胞數的比值差異無統計學意義(P＞0.05).結論 人工建立褪黑素節律對婦科手術病入術後細胞免疫功能無明顯影響.
목적 평개인공건립퇴흑소절률대부과수술병인술후세포면역공능적영향.방법 택기행부과수술병인18례,년령25 ～ 50세,체중45 ～ 80 kg,ASA분급Ⅰ혹Ⅱ급,채용수궤수자표법,장기수궤분위3조(n=6):정상대조조(C조)、안위제대조조(P조)화퇴흑소조(M조)、분별우술전1d、수술당천화술후1 d21:00식등전10 min시,M조구복퇴흑소6 mg,P조구복안위제.술중채용련속경막외마취,술후채용병인자공경막외진통,유지VAS평분＜5분.분별우술전1d(기출상태)、술필급술후1d시채집외주정맥혈양,채용류식세포술측정CD4+、CD8+、CD3+세포수,병계산CD4+여CD8+세포수적비치.결과 C조、P조화M조간CD4+、CD8+、CD3+세포수급CD4+여CD8+세포수적비치차이무통계학의의(P＞0.05).결론 인공건립퇴흑소절률대부과수술병입술후세포면역공능무명현영향.
Objective To investigate the effect of artifictal circadian rhythm of melatonin on the postoperative cellular immune function in patients undergoing gynecological operation Methods Eighteen ASA Ⅰ or Ⅱ patients,aged 25-50 yr,weighing 45-80 kg,scheduled for elective gynecological operation,were randomly divided into 3 groups (n =6 each):control group (group C),placebo control group (group P) and melatonin group (group M).In group M.melatonin 6 mg was given orally at 10 min before lights-out (21:00) on 1 day before operation,on the day of operation and on 1 day after operation,while placebo was given orally instead of melatonin in group P.The operation was performed under epidural anesthesia.Patient-controlled epidural analgesia with ropivacaine was used for postoperative analgesia.VAS score was maintained ＜ 5.Blood samples were collected from the peripheral vein at 1 day before operation (baseline),the end of operation and 1 day after operation to measure CD4+,CD8+ and CD3+ cell count by flow cytometry.The ratio of the number of CD4+ cells to the number of CD8+ cells was calculated.Results There were no significant differences in the number of CD4+,CD8+ and CD3 + cells and ratio of the number of CD4 + cells to the number of CD8 + cells between groups C,P and M (P ＞0.05).Conclusion Artificial circadian rhythm of melatonin exerts no influence on the postoperative cellular immune function in patients undergoing gynecological operation.
