CAJ | 학술논문

目的评价大肠杆菌密度感应调节子C(qseC)在失血性休克大鼠肠道细菌移位中的作用.方法成年雄性SD大鼠30只,体重250 ～ 300 g,采用随机数字表法,将大鼠随机分为5组(n=6):对照组(C组)、MC1000-假休克组(M-SS组)、MC1000△qseC-假休克组(△-SS组)、MC1000-休克组(M-HS组)和MC1000△qseC-休克组(△-HS组).大鼠连续3d饮用150 μg/ml链霉素的消毒水溶液,抑制肠道内固有菌群；C组第4天开始,连续3d灌饲生理盐水(1 ml/100 g,1次/d),其余4组第4天开始,连续3d分别灌饲大肠杆菌MC1000或MC1000△qseC菌液(1 ml/100 g,1次/d).采用左股动脉放血法制备失血性休克模型.手术结束后24 h时采用链霉素抗性特点和菌落PCR技术对从内脏分离出的细菌进行鉴定；观察细菌移位情况和计数内脏组织细菌数.结果与C组比较,M-HS组和△-HS组大鼠细菌移位率、肠系膜淋巴结细菌含量、脾脏和肝脏菌落数和总菌落数均升高(P＜0.05)；与M-SS组和△-SS组比较,M-HS组大鼠细菌移位率、肠系膜淋巴结细菌含量、脾脏和肝脏菌落数和总菌落数均升高(P＜0.05)；与M-HS组比较,△-HS组大鼠细菌移位率、肠系膜淋巴结、脾脏和肝脏菌落数和总菌落数均降低(P＜0.05).结论大肠杆菌qseC参与了失血性休克大鼠肠道细菌移位的过程.
목적 평개대장간균밀도감응조절자C(qseC)재실혈성휴극대서장도세균이위중적작용.방법 성년웅성SD대서30지,체중250 ～ 300 g,채용수궤수자표법,장대서수궤분위5조(n=6):대조조(C조)、MC1000-가휴극조(M-SS조)、MC1000△qseC-가휴극조(△-SS조)、MC1000-휴극조(M-HS조)화MC1000△qseC-휴극조(△-HS조).대서련속3d음용150 μg/ml련매소적소독수용액,억제장도내고유균군；C조제4천개시,련속3d관사생리염수(1 ml/100 g,1차/d),기여4조제4천개시,련속3d분별관사대장간균MC1000혹MC1000△qseC균액(1 ml/100 g,1차/d).채용좌고동맥방혈법제비실혈성휴극모형.수술결속후24 h시채용련매소항성특점화균락PCR기술대종내장분리출적세균진행감정；관찰세균이위정황화계수내장조직세균수.결과 여C조비교,M-HS조화△-HS조대서세균이위솔、장계막림파결세균함량、비장화간장균락수화총균락수균승고(P＜0.05)；여M-SS조화△-SS조비교,M-HS조대서세균이위솔、장계막림파결세균함량、비장화간장균락수화총균락수균승고(P＜0.05)；여M-HS조비교,△-HS조대서세균이위솔、장계막림파결、비장화간장균락수화총균락수균강저(P＜0.05).결론 대장간균qseC삼여료실혈성휴극대서장도세균이위적과정.
Objective To investigate the role of quorum sensing Escherichia coli regulator C (qseC) in intestinal bacterial translocation in rats subjected to hemorrhagic shock.Methods Thirty Sprague-Dawley rats,weighing 250-300 g,were randomly divided into 5 groups (n =6 each):control group (group C),MC1000-sham shock group (group M-SS),MC1000qseC-sham shock group (group △-SS),MC1000-hemorrhagic shock group (group M-HS),and MC1000△ qseC-hemorrhagic shock group (group △-HS).The rats drank 150 μg/ml of disinfect water containing streptomycin in 3 consecutive days to inhibit the autochthonous flora in the intestinal tract.From 4th day,the rats were fed with Escherichia Coli MC1000 or MC1000△ qseC 1 ml/100 g by gastric perfusion once a day for another 3 consecutive days in the other 4 groups,while the rats were fed with normal saline instead in group C.Hemorrhagic shock was induced by blood-letting.The mesenteric lymph node (MLN),spleen and liver specimens were obtained at 24 h after operation for bacterial culture and the bacteria were identified.Bacterial translocation from gut to MLN,spleen and liver was observed and the number of bacteria in MLN,spleen and liver tissues were counted.Results The rate of bacterial translocation was significantly higher,and the number of bacterial colonies in MLN,spleen and liver tissues and the total number of bacterial colonies were significantly larger in groups M-HS and △-HS than in group C,and in group M-HS than in groups M-SS and △-SS (P ＜ 0.05).The rate of bacterial translocation was significantly lower,and the number of bacterial colonies in MLN,spleen and liver tissues and the total number of bacterial colonies were significantly smaller in group △-HS than in group MHS.Conclusion QseC is involved in the intestinal bacterial translocation following hemorrhagic shock in rats.