中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2012年
12期
1437-1440
,共4页
硫辛酸%心肺转流术%糖尿病%认知障碍
硫辛痠%心肺轉流術%糖尿病%認知障礙
류신산%심폐전류술%당뇨병%인지장애
Thioctic acid%Cardiopulmonary bypass%Diabetes mellitus%Cognition disorders
目的 评价α-硫辛酸对糖尿病大鼠体外循环(CPB)后认知功能的影响.方法 成年雄性SD大鼠,体重400～ 450 g,16 ～ 22周龄,腹腔注射1％链脲佐菌素60 mg/kg建立糖尿病模型.取糖尿病模型制备成功的大鼠32只,采用随机数字表法,将其随机分为2组(n=16):糖尿病组(D组)和α-硫辛酸组(L组).L组在糖尿病模型建立后第6周腹腔注射α-硫辛酸30 mg/kg,1次/d,连续7d,D组给予等容量生理盐水.2组于停止腹腔给药后进行CPB.分别于糖尿病模型建立前、糖尿病模型建立后第5周、CPB模型建立前、CPB结束时、CPB停止后3d及CPB停止后5d时取10只大鼠,采集静脉血样,测定血浆TNF-α及IL-10的浓度；分别于糖尿病模型建立前、CPB模型建立前和CPB停止后5d时取10只大鼠,测定认知功能,认知功能测试后处死,取海马组织,测定神经元NF-κB活性.结果 与D组比较,L组血浆TNF-α浓度、电击次数和海马组织神经元NF-κB活性降低,血浆IL-10浓度升高(P＜ 0.05或0.01).结论 α-硫辛酸可改善糖尿病大鼠CPB后认知功能,其机制与抑制海马神经元NF-κB激活有关.
目的 評價α-硫辛痠對糖尿病大鼠體外循環(CPB)後認知功能的影響.方法 成年雄性SD大鼠,體重400～ 450 g,16 ～ 22週齡,腹腔註射1％鏈脲佐菌素60 mg/kg建立糖尿病模型.取糖尿病模型製備成功的大鼠32隻,採用隨機數字錶法,將其隨機分為2組(n=16):糖尿病組(D組)和α-硫辛痠組(L組).L組在糖尿病模型建立後第6週腹腔註射α-硫辛痠30 mg/kg,1次/d,連續7d,D組給予等容量生理鹽水.2組于停止腹腔給藥後進行CPB.分彆于糖尿病模型建立前、糖尿病模型建立後第5週、CPB模型建立前、CPB結束時、CPB停止後3d及CPB停止後5d時取10隻大鼠,採集靜脈血樣,測定血漿TNF-α及IL-10的濃度；分彆于糖尿病模型建立前、CPB模型建立前和CPB停止後5d時取10隻大鼠,測定認知功能,認知功能測試後處死,取海馬組織,測定神經元NF-κB活性.結果 與D組比較,L組血漿TNF-α濃度、電擊次數和海馬組織神經元NF-κB活性降低,血漿IL-10濃度升高(P＜ 0.05或0.01).結論 α-硫辛痠可改善糖尿病大鼠CPB後認知功能,其機製與抑製海馬神經元NF-κB激活有關.
목적 평개α-류신산대당뇨병대서체외순배(CPB)후인지공능적영향.방법 성년웅성SD대서,체중400～ 450 g,16 ～ 22주령,복강주사1％련뇨좌균소60 mg/kg건립당뇨병모형.취당뇨병모형제비성공적대서32지,채용수궤수자표법,장기수궤분위2조(n=16):당뇨병조(D조)화α-류신산조(L조).L조재당뇨병모형건립후제6주복강주사α-류신산30 mg/kg,1차/d,련속7d,D조급여등용량생리염수.2조우정지복강급약후진행CPB.분별우당뇨병모형건립전、당뇨병모형건립후제5주、CPB모형건립전、CPB결속시、CPB정지후3d급CPB정지후5d시취10지대서,채집정맥혈양,측정혈장TNF-α급IL-10적농도；분별우당뇨병모형건립전、CPB모형건립전화CPB정지후5d시취10지대서,측정인지공능,인지공능측시후처사,취해마조직,측정신경원NF-κB활성.결과 여D조비교,L조혈장TNF-α농도、전격차수화해마조직신경원NF-κB활성강저,혈장IL-10농도승고(P＜ 0.05혹0.01).결론 α-류신산가개선당뇨병대서CPB후인지공능,기궤제여억제해마신경원NF-κB격활유관.
Objective To evaluate the effect of α-lipoic acid on the cognitive function after cardiopulmonary bypass (CPB) in diabetic rats.Methods Health adult male Sprague-Dawley rats,weighing 400-500 g,aged 16-22 weeks,were used in this study.Diabetes mellitus was induced by intraperitoneal streptozocin 60 mg/kg and confirmed by blood glucose≥ 16.7 mmol/L.Thirty-two diabetic rats were randomly divided into 2 groups (n =16 each):diabetes mellitus group (group D) and α-lipoi cacid group (group L).In group L,α-lipoic acid 30 mg/kg was intraperitoneally injected once a day for 7 consecutive days starting from 6th week after induction of diabetes mellitus.While the equal volume of normal saline was given instead in group D.The two groups underwent CPB after the last administration.Before induction of diabetes mellitus,on 5th week after induction of diabetes mellitus,before CPB,at the end of CPB,and on 3 and 5 days after termination of CPB,10 rats were chosen from each group and venous blood samples were collected for determination of plasma TNF-α and IL-10 concentrations.Ten rats in each group were chosen for detection of cognitive function before induction of diabetes mellitus,before CPB and 5 days after termination of CPB.The rats were then sacrificed and hippocampi were isolated for measurement of NF-κB activity.Results Compare with group D,the plasma TNF-α concentration,times of electric shock and activity of NF-κB in hippocampal tissues were significantly decreased and the plasma IL-10 concentration was increased in group L (P ＜ 0.05 or 0.01).Conclusion α-lipoic acid can improve the cognitive function after CPB in diabetic rats and inhibition of activation of NF-κB in hippocampal neurons is involved in the mechanism.
