中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2012年
12期
1485-1487
,共3页
胆碱能拮抗剂%内毒素类%呼吸窘迫综合征,成人%缺氧诱导因子1,α亚基
膽堿能拮抗劑%內毒素類%呼吸窘迫綜閤徵,成人%缺氧誘導因子1,α亞基
담감능길항제%내독소류%호흡군박종합정,성인%결양유도인자1,α아기
Cholinergic antagonists%Endotoxins%Respiratory distress syndrome,adult%Hypoxia-inducible factor 1,alpha subunit
目的 评价盐酸戊乙奎醚预先给药对大鼠内毒素性急性肺损伤时缺氧诱导因子-1α(HIF-1α)表达的影响.方法 健康成年雌性SD大鼠120只,体重180 ～ 220 g,采用随机数字表法,将大鼠随机分为3组(n=40):对照组(C组)、急性肺损伤组(ALI组)和盐酸戊乙奎醚预先给药组(P组).采用腹腔注射内毒素5 mg/kg制备大鼠内毒素性急性肺损伤模型.C组腹腔注射等量生理盐水,P组于注射内毒素前30 min时腹腔注射盐酸戊乙奎醚2 mg/kg.于注射内毒素后2、4、8和24 h时各组随机取8只大鼠处死取肺,采用RT-PCR法检测肺组织HIF-1α mRNA的表达.于注射内毒素后6h时随机取8只大鼠处死取肺,测定湿干重比(W/D比),用ELISA法检测肺组织IL-6的含量,光镜下观察肺组织病理学结果.结果 与C组比较,ALI组和P组注射内毒素后6h时W/D比、IL-6含量、各时点HIF-1α表达水平升高(P＜0.05)；与ALI组比较,P组注射内毒素后6h时W/D比、IL-6含量、各时点HIF-1α表达水平降低(P＜0.05).P组肺组织病理学损伤程度较ALI组减轻.结论 盐酸戊乙奎醚预先给药通过下调肺组织HIF-1α表达,抑制炎性反应,从而减轻大鼠内毒素性急性肺损伤.
目的 評價鹽痠戊乙奎醚預先給藥對大鼠內毒素性急性肺損傷時缺氧誘導因子-1α(HIF-1α)錶達的影響.方法 健康成年雌性SD大鼠120隻,體重180 ～ 220 g,採用隨機數字錶法,將大鼠隨機分為3組(n=40):對照組(C組)、急性肺損傷組(ALI組)和鹽痠戊乙奎醚預先給藥組(P組).採用腹腔註射內毒素5 mg/kg製備大鼠內毒素性急性肺損傷模型.C組腹腔註射等量生理鹽水,P組于註射內毒素前30 min時腹腔註射鹽痠戊乙奎醚2 mg/kg.于註射內毒素後2、4、8和24 h時各組隨機取8隻大鼠處死取肺,採用RT-PCR法檢測肺組織HIF-1α mRNA的錶達.于註射內毒素後6h時隨機取8隻大鼠處死取肺,測定濕榦重比(W/D比),用ELISA法檢測肺組織IL-6的含量,光鏡下觀察肺組織病理學結果.結果 與C組比較,ALI組和P組註射內毒素後6h時W/D比、IL-6含量、各時點HIF-1α錶達水平升高(P＜0.05)；與ALI組比較,P組註射內毒素後6h時W/D比、IL-6含量、各時點HIF-1α錶達水平降低(P＜0.05).P組肺組織病理學損傷程度較ALI組減輕.結論 鹽痠戊乙奎醚預先給藥通過下調肺組織HIF-1α錶達,抑製炎性反應,從而減輕大鼠內毒素性急性肺損傷.
목적 평개염산무을규미예선급약대대서내독소성급성폐손상시결양유도인자-1α(HIF-1α)표체적영향.방법 건강성년자성SD대서120지,체중180 ～ 220 g,채용수궤수자표법,장대서수궤분위3조(n=40):대조조(C조)、급성폐손상조(ALI조)화염산무을규미예선급약조(P조).채용복강주사내독소5 mg/kg제비대서내독소성급성폐손상모형.C조복강주사등량생리염수,P조우주사내독소전30 min시복강주사염산무을규미2 mg/kg.우주사내독소후2、4、8화24 h시각조수궤취8지대서처사취폐,채용RT-PCR법검측폐조직HIF-1α mRNA적표체.우주사내독소후6h시수궤취8지대서처사취폐,측정습간중비(W/D비),용ELISA법검측폐조직IL-6적함량,광경하관찰폐조직병이학결과.결과 여C조비교,ALI조화P조주사내독소후6h시W/D비、IL-6함량、각시점HIF-1α표체수평승고(P＜0.05)；여ALI조비교,P조주사내독소후6h시W/D비、IL-6함량、각시점HIF-1α표체수평강저(P＜0.05).P조폐조직병이학손상정도교ALI조감경.결론 염산무을규미예선급약통과하조폐조직HIF-1α표체,억제염성반응,종이감경대서내독소성급성폐손상.
Objective To investigate the effects of penehyclidine hydrochloride (PHCD) pretreatment on hypoxia-inducible factor-1alpha (HIF-1α) expression in lung tissues in rats with acute lung injury (ALI) induced by lipopolysaccharide (LPS).Methods One hundred and twenty female Sprague-Dawley rats,weighing 180-220g,were randomly divided into 3 groups (n =40 each):control group (group C),ALI group and PHCD pretreatment group (group P).ALI was induced by intraperitoneal LPS 5 mg/kg in ALI and P groups.The equal volume of normal saline was given intraperitoneally in group C.Group P received intraperitoneal PHCD 2 mg/kg at 30 min before LPS administration.Eight rats in each group were chosen at 2,4,8 and 24 h after LPS administration,and the lung was removed for determination of HIF-1α mRNA expression in lung tissues by RT-PCR.Eight rats in each group were chosen at 6 h after LPS administration,and the lung was removed for determination of W/D lung weight ratio and IL-6 content (by ELISA) and for microscopic examination.Results The W/D lung weight ratio and IL-6 content at 6 h after LPS administration and HIF-1α mRNA expression at each time point after LPS administration were significantly higher in groups P and ALI than in group C (P ＜ 0.05),and lower in group P than in group ALI(P ＜ 0.05).The pathological damage was significantly ameliorated in group P as compared with group ALI.Conclusion PHCD can reduce LPS-induced ALI through down-regulation of HIF-1α expression in lung tissues and inhibition of the inflammatory response in rats.
