CAJ | 학술논문

目的评价含饱和氢气肾保存液对大鼠肾脏冷缺血再灌注损伤的影响.方法健康雄性Wistar大鼠24只,周龄8～10周,体重200～ 250 g,采用随机数字表法,将其随机分为3组(n=8):对照组(H1组)大鼠仅切除右肾；普通肾保存液组(H2组)大鼠采用冷缺血再灌注模型,用4℃普通HC-A肾保存液对左肾行冷灌注和冷保存；含饱和氢气肾保存液组(H3组)大鼠操作同H2组,灌注液及保存液换用自制的4℃含饱和氢气HC-A肾保存液.于再灌注24 h时抽取下腔静脉血样,测定血清BUN、Cr、TNF-α和IL-6浓度；切取左肾,测定肾组织MDA和8-羟基脱氧鸟苷(8-OHdG)含量,光镜下观察肾组织病理学结果.结果与H1组相比,H2组和H3组大鼠血清BUN、Cr、TNF-α和IL-6浓度及肾组织MDA和8-OHdG含量均升高(P＜ 0.05);与H2组相比,H3组血清BUN、Cr、TNF-α和IL-6浓度及肾组织MDA和8-OHdG的含量均降低(P＜0.05).H1组肾组织形态结构未见明显异常,H2组肾小管损伤明显,H3组肾小管损伤较H2组减轻.结论含饱和氢气肾保存液可明显减轻大鼠肾脏冷缺血再灌注损伤.
목적 평개함포화경기신보존액대대서신장랭결혈재관주손상적영향.방법 건강웅성Wistar대서24지,주령8～10주,체중200～ 250 g,채용수궤수자표법,장기수궤분위3조(n=8):대조조(H1조)대서부절제우신；보통신보존액조(H2조)대서채용랭결혈재관주모형,용4℃보통HC-A신보존액대좌신행랭관주화랭보존；함포화경기신보존액조(H3조)대서조작동H2조,관주액급보존액환용자제적4℃함포화경기HC-A신보존액.우재관주24 h시추취하강정맥혈양,측정혈청BUN、Cr、TNF-α화IL-6농도；절취좌신,측정신조직MDA화8-간기탈양조감(8-OHdG)함량,광경하관찰신조직병이학결과.결과 여H1조상비,H2조화H3조대서혈청BUN、Cr、TNF-α화IL-6농도급신조직MDA화8-OHdG함량균승고(P＜ 0.05);여H2조상비,H3조혈청BUN、Cr、TNF-α화IL-6농도급신조직MDA화8-OHdG적함량균강저(P＜0.05).H1조신조직형태결구미견명현이상,H2조신소관손상명현,H3조신소관손상교H2조감경.결론 함포화경기신보존액가명현감경대서신장랭결혈재관주손상.
Objective To investigate the effects of hydrogen-rich HC-A solution,the self-made kidney preservation solution,on renal cold ischemia/reperfusion (I/R) injury in rats.Methods Twenty-four healthy male Wistar rats,aged 8-10 weeks,weighing 200-250 g,were randomly divided into 3 groups (n =8 each):control group (H1 group),common kidney preservation solution group (H2 group) and hydrogen-rich HC-A kidney preservation solution group (H3 group).In H1 group,only the right kidney was removed.In H2 group,the left kidney was perfused with and cold stored in 4 ℃ common HC-A kidney preservation solution.In H3 group,the left kidney was perfused with and cold stored in a container filled with 4 ℃ common HC-A kidney preservation solution.Blood samples were obtained from the inferior vena cava at 24 h of reperfusion to detect the levels of serum blood urea nitrogen (BUN),creatinine (Cr),TNF-α and IL-6.Left kidneys were removed for determination of malondialdehyde (MDA) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) contents and for examination of the pathological changes in renal tissues (by light microscopy).Results The levels of serum BUN,Cr,TNF-α and IL-6 and contents of MDA and 8-OHdG were significantly higher in H2 and H3 groups than in H1 group,and lower in H3 group than in H2 group (P ＜ 0.05).There were no significant pathological changes in renal tissues in H1 group,the damage to renal tubules was obvious in H2 group and the damage to renal tubules was significantly ameliorated in H3 group as compared with H2 group.Conclusion Hydrogen-rich kidney preservation solution can attenuate renal cold I/R injury in rats.