CAJ | 학술논문

目的评价利多卡因致大鼠惊厥时海马神经元含NR2B亚基的N-甲基-D-天门冬氨酸(NMDA)受体表达的变化.方法健康雄性SD大鼠24只,体重220～250 g,6月龄,采用随机数字表发法,将其分为2组(n=12):生理盐水组(N组)和利多卡因组(L组).L组经尾静脉输注2％利多卡因4 mg· kg-1·min-1至大鼠发生RacineⅢ级惊厥,N组以等容量等速率生理盐水替代.于惊厥停止后即刻、6、12 h(T1～3)时取海马,采用免疫印迹和RT-PCR法检测NR2B蛋白及mRNA的表达水平.结果与N组比较,L组NR2B蛋白及mRNA表达上调.与T1时比较,L组T2,3时NR2B蛋白及mRNA表达下调(P＜0.05).与T2时比较,L组T3时NR2B蛋白表达下调(P＜0.05).结论利多卡因诱发大鼠惊厥时海马神经元含NR2B亚基的NMDA受体表达上调,该变化可能与利多卡因诱发惊厥发作有关.
목적 평개리다잡인치대서량궐시해마신경원함NR2B아기적N-갑기-D-천문동안산(NMDA)수체표체적변화.방법 건강웅성SD대서24지,체중220～250 g,6월령,채용수궤수자표발법,장기분위2조(n=12):생리염수조(N조)화리다잡인조(L조).L조경미정맥수주2％리다잡인4 mg· kg-1·min-1지대서발생RacineⅢ급량궐,N조이등용량등속솔생리염수체대.우량궐정지후즉각、6、12 h(T1～3)시취해마,채용면역인적화RT-PCR법검측NR2B단백급mRNA적표체수평.결과 여N조비교,L조NR2B단백급mRNA표체상조.여T1시비교,L조T2,3시NR2B단백급mRNA표체하조(P＜0.05).여T2시비교,L조T3시NR2B단백표체하조(P＜0.05).결론 리다잡인유발대서량궐시해마신경원함NR2B아기적NMDA수체표체상조,해변화가능여리다잡인유발량궐발작유관.
Objective To evaluate the changes in the expression of NR2B subunit-containing N-methyl-D-aspartate receptors in hippocampal neurons of rats with lidocaine-induced convulsion.Methods Twenty-four male Sprague-Dawley rats,aged 6 months,weighing 220-250 g,were randomly divided into 2 groups (n =12 each):normal saline group (group N) and lidocaine group (group L).In L group,2％ lidocaine was injected via the tail vein at a rate of 4 mg· kg-1· min-1 until the animals developed convulsion (Racine Ⅲ).The equal volume of normal saline was given instead in group N.Hippocampi were isolated at 0,6 and 12 h after convulsion stopped (T1-3) to detect the expression of NR2B protein and mRNA using Western blot and RT-PCR.Results Compared with group N,NR2B protein and mRNA expression was significantly up-regulated in group L (P ＜ 0.05).NR2B protein and mRNA expression was significantly lower at T1 than at T2 and T3 in group L (P ＜ 0.05).NR2B protein expression was significantly lower at T3 than at T2 in group L (P ＜ 0.05).Conclusion The expression of NR2B subunit-containing N-methyl-D-aspartate receptors is up-regulated in hippocampal neurons of rats with lidocaine-induced convulsion,and the change may be due to convulsion induced by lidocaine.