CAJ | 학술논문

目的评价依达拉奉后处理联合远隔缺血后处理对大鼠心肌缺血再灌注损伤的影响.方法健康雄性SD大鼠40只,8周龄,体重250～300 g,采用随机数字表法,将其分为5组(n=8):假手术组(S组)、缺血再灌注组(I/R组)、依达拉奉后处理组(E组)、远隔缺血后处理组(P组)、依达拉奉联合远隔缺血后处理组(EP组).采用结扎左冠状动脉前降支30 min,再灌注180 min制备心肌缺血再灌注模型.E组和EP组再灌注前1 min静脉注射依达拉奉3mg/kg;P组和EP组左冠状动脉结扎20 min时实施远隔后处理:用止血带结扎大鼠双后肢,持续10 min.于心肌缺血再灌注期间记录左心室峰压(LVSP)、左心室舒张末压(LVEDP)、左室内压最大上升速率(+dp/dtmax)、左室内压最大下降速率(-dp/dtmax).结果与S组比较,其它各组再灌注期间LVSP、+dp/dtmax、-dp/dtmax降低,LVEDP升高(P＜0.05)；与I/R组比较,E组、P组及EP组再灌注期间LVSP、+dp/dtmax、-dp/dtmax升高,LVEDP降低(P＜0.05)；与E组和P组比较,EP组再灌注期间LVSP、+dp/dtmax、-dp/dtmax升高,LVEDP降低(P＜0.05).结论依达拉奉后处理联合远隔缺血后处理可减轻心肌缺血再灌注损伤,且效果强于两者单独应用.
목적 평개의체랍봉후처리연합원격결혈후처리대대서심기결혈재관주손상적영향.방법 건강웅성SD대서40지,8주령,체중250～300 g,채용수궤수자표법,장기분위5조(n=8):가수술조(S조)、결혈재관주조(I/R조)、의체랍봉후처리조(E조)、원격결혈후처리조(P조)、의체랍봉연합원격결혈후처리조(EP조).채용결찰좌관상동맥전강지30 min,재관주180 min제비심기결혈재관주모형.E조화EP조재관주전1 min정맥주사의체랍봉3mg/kg;P조화EP조좌관상동맥결찰20 min시실시원격후처리:용지혈대결찰대서쌍후지,지속10 min.우심기결혈재관주기간기록좌심실봉압(LVSP)、좌심실서장말압(LVEDP)、좌실내압최대상승속솔(+dp/dtmax)、좌실내압최대하강속솔(-dp/dtmax).결과 여S조비교,기타각조재관주기간LVSP、+dp/dtmax、-dp/dtmax강저,LVEDP승고(P＜0.05)；여I/R조비교,E조、P조급EP조재관주기간LVSP、+dp/dtmax、-dp/dtmax승고,LVEDP강저(P＜0.05)；여E조화P조비교,EP조재관주기간LVSP、+dp/dtmax、-dp/dtmax승고,LVEDP강저(P＜0.05).결론 의체랍봉후처리연합원격결혈후처리가감경심기결혈재관주손상,차효과강우량자단독응용.
Objective To evaluate the effects of edaravone postconditioning and remote ischemic postconditioning on myocardial ischemia/reperfusion (I/R) injury in rats.Methods Forty male Sprague-Dawley rats,aged 8 weeks,weighing 250-300 g,were randomly divided into 5 groups (n =8 each):sham operation group (group S); group I/R; edaravone postconditioning group (group E); remote ischemic postconditioning group (group P); edaravone postconditioning and remote ischemic postconditioning group (group EP).Myocardial I/R was induced by occlusion of anterior desending branch of left coronary artery for 30 min followed by 180 min reperfusion.Edaravone 3 mg/kg was injected intravenously at 1 min before reperfusion in groups E and EP.The animals underwent 10 min ischemia of bilateral hind limbs starting from 20 min of myocardial ischemia in groups P and EP.Left ventricular systolic pressure (LVSP),left ventricular end-diastolic pressure (LVEDP) and ± dp/dtmax were measured and recorded during reperfusion.Results Compared with group S,LVSP and ± dp/dtmax were significantly decreased and LVEDP was increased in the other groups (P ＜ 0.05).LVSP and ± dp/dtmax were significantly higher and LVEDP was lower during reperfusion in groups E,P and EP than in group I/R,and in group EP than in groups E and P (P ＜ 0.05).Conclusion Edaravone postconditioning and remote ischemic postconditioning can alleviate myocardial I/R injury and offers better efficacy than either alone.