CAJ | 학술논문

目的评价背根神经节小胶质细胞活化在大鼠术后持续性痛形成中的作用.方法成年雄性SD大鼠70只,体重200 ～ 250 g,采用皮肤肌肉切口牵拉法建立术后持续性痛模型,采用随机数字表法,将其分为2组(n=35):假手术组(S组)和术后持续性痛组(SMIR组).分别于术前ld、术后1、3、7、12、22和32 d时,测定机械痛阈.上述各时点痛阈测试完毕后,随机取5只大鼠,计数背根神经节小胶质细胞.结果与S组比较,SMIR组术后3～22 d时机械痛阈降低,术后3～12d时背根神经节小胶质细胞计数升高(P＜0.05).结论背根神经节小胶质细胞的活化可能参与了大鼠术后持续性痛的形成.
목적 평개배근신경절소효질세포활화재대서술후지속성통형성중적작용.방법 성년웅성SD대서70지,체중200 ～ 250 g,채용피부기육절구견랍법건립술후지속성통모형,채용수궤수자표법,장기분위2조(n=35):가수술조(S조)화술후지속성통조(SMIR조).분별우술전ld、술후1、3、7、12、22화32 d시,측정궤계통역.상술각시점통역측시완필후,수궤취5지대서,계수배근신경절소효질세포.결과 여S조비교,SMIR조술후3～22 d시궤계통역강저,술후3～12d시배근신경절소효질세포계수승고(P＜0.05).결론 배근신경절소효질세포적활화가능삼여료대서술후지속성통적형성.
Objective To evaluate the role of microglial activation in dorsal root ganglia in a rat model of persistent postoperative pain evoked by skin/muscle incision and retraction (SMIR).Methods Seventy male Sprague-Dawley rats,weighing 200-250 g,were randomly divided into 2 groups (n =35 each):group sham operation (group S) and group SMIR.The rat model of persistent postoperative pain evoked by SMIR was established according to the method described by Flatters.Pain behavior was assessed by mechanical paw withdrawal threshold to yon Frey filament stimulation at 1 day before and 1,3,7,12,22 and 32 days after operation.Five animals were sacrificed at each time point in each group for microglia count in dorsal root ganglia.Results Compared with group S,mechanical paw withdrawal threshold was significantly decreased at 3-22 days after operation,and microglia count was significantly increased at 3-12 days after operation in group SMIR (P ＜ 0.05).Conclusion Microglial activation in dorsal root ganglia may be involved in the development of SMIR-evoked persistent postoperative pain in rats.