CAJ | 학술논문

目的探讨不同压力高压氧治疗(HBO)对大鼠神经病理性痛的影响.方法成年雄性SD大鼠24只,采用随机数字表法,将其分为4组(n=6):假手术组(S组)、神经病理性痛组(NP组)、2.O标准大气压高压氧治疗组(HBO2.0组)、2.5标准大气压高压氧治疗组(HBO2.5组).将HBO2.0组和HBO2.5组大鼠置于高压氧舱,以10 kPa/min的速率向舱内匀速加压直至目标压力并保持60 min.然后以10 kPa/min的速率匀速减压至正常大气压.从术后1d开始高压氧治疗,连续5d,1次/d.S组和NP组单纯放入氧舱100 min而不接受治疗.于出高压氧舱后即刻(T0)、1 h(T1)和2 h(T2)时测定机械缩足反应阈(MWT)和热缩足反应潜伏期(TWL).结果与S组比较,NP组MWT降低,TWL缩短(P＜0.05).与NP组比较,HBO2.0组和HBO2.5组高压氧治疗期间T1时MWT升高,TWL延长(P＜0.05),T2时MWT和TWL差异无统计学意义(P＞0.05).HBO2.0组和HBO2.5组间各时点MWT和TWL比较差异无统计学意义(P＜0.05).结论不同压力高压氧治疗均可减轻大鼠神经病理性痛,但压力改变不影响镇痛效果.
목적 탐토불동압력고압양치료(HBO)대대서신경병이성통적영향.방법 성년웅성SD대서24지,채용수궤수자표법,장기분위4조(n=6):가수술조(S조)、신경병이성통조(NP조)、2.O표준대기압고압양치료조(HBO2.0조)、2.5표준대기압고압양치료조(HBO2.5조).장HBO2.0조화HBO2.5조대서치우고압양창,이10 kPa/min적속솔향창내균속가압직지목표압력병보지60 min.연후이10 kPa/min적속솔균속감압지정상대기압.종술후1d개시고압양치료,련속5d,1차/d.S조화NP조단순방입양창100 min이불접수치료.우출고압양창후즉각(T0)、1 h(T1)화2 h(T2)시측정궤계축족반응역(MWT)화열축족반응잠복기(TWL).결과 여S조비교,NP조MWT강저,TWL축단(P＜0.05).여NP조비교,HBO2.0조화HBO2.5조고압양치료기간T1시MWT승고,TWL연장(P＜0.05),T2시MWT화TWL차이무통계학의의(P＞0.05).HBO2.0조화HBO2.5조간각시점MWT화TWL비교차이무통계학의의(P＜0.05).결론 불동압력고압양치료균가감경대서신경병이성통,단압력개변불영향진통효과.
Objective To investigate the effect of different pressures of hyperbaric oxygen (HBO) treatment on neuropathic pain (NP) in rats.Methods Twenty-four healthy male Sprague-Dawley rats were randomly divided into 4 groups (n =6 each):sham operation group (group S),NP group,treatment with HBO at 2.0 atmosphere absolute group (group HBO2.0) and treatment with HBO at 2.5 atmosphere absolute group (group HBO2.5).The animals were anesthetized with intraperitoneal 5 ％ chloral hydrate 300 mg/kg.NP was induced by chronic constrictive injury.The left sciatic nerve was exposed and 4 loose ligatures were placed on the sciatic nerve at 1-mm intervals with 4-0 silk thread.After the rats were placed in the HBO chamber,the pressure was increased at a rate of 10 kPa/min until the desired pressure was reached,and then the pressure was maintained at this level for 60 min.The pressure was then decreased at a rate of 10 kPa/min until the normal pressure was reached.HBO treatment was performed once a day for 5 consecutive days starting from 1st day after operation in groups HBO2.0 and HBO2.5.While the rats were only placed in the HBO chamber and stayed for 100 min in groups S and NP.The mechanical paw withdrawal threshold (MWT) and thermal paw withdrawal latency (TWL) were measured at 0,1and 2 h after leaving the HBO chamber (T0.2).Results Compared with group S,MWT was significantly decreased and TWL was shortened in group NP (P ＜ 0.05).Compared with group NP,MWT was significantly increased and TWL prolonged at T1 during the treatment (P ＜ 0.05),while no significant change was found in MWT and TWL at T2 during the treatment in groups HBO2.0 and HBO2.5 (P ＞ 0.05).There was no significant difference in MWT and TWL at each time point during the treatment between groups HBO2.0 and HBO2.5 (P ＞ 0.05).Conclusion Treatment with different pressures of HBO can reduce NP in rats,but the change in pressure dose not affect the analgesic efficacy.