CAJ | 학술논문

目的评价手术创伤对老龄大鼠认知功能及海马铁调素和膜铁转运蛋白1(FP1)表达的影响.方法健康老龄雄性SD大鼠100只,18月龄,体重400～ 500 g,采用随机数字表法,将其分为2组(n=50):对照组(C组)腹腔注射水合氯醛麻醉,但不进行手术；手术创伤组(ST组)腹腔注射水合氯醛麻醉后行改良性剖腹探查术30 min.于术后24h时随机取10只大鼠,行水迷宫实验,测定认知功能,持续6d,分别于水迷宫实验第1、3、5、7天时随机取10只大鼠处死,取脑,分离海马,采用PCR和Western blot法检测海马铁调素和FP1的表达.结果与C组比较,ST组水迷宫实验第3、4、5天时大鼠逃避潜伏期延长,原平台象限停留时间缩短,穿越原平台次数减少(P＜0.05),各时点海马铁调素表达上调,FP1表达下调(P＜0.05).结论手术创伤可降低老龄大鼠认知功能,其机制可能与上调海马铁调素表达,下调FP1表达有关.
목적 평개수술창상대노령대서인지공능급해마철조소화막철전운단백1(FP1)표체적영향.방법 건강노령웅성SD대서100지,18월령,체중400～ 500 g,채용수궤수자표법,장기분위2조(n=50):대조조(C조)복강주사수합록철마취,단불진행수술；수술창상조(ST조)복강주사수합록철마취후행개량성부복탐사술30 min.우술후24h시수궤취10지대서,행수미궁실험,측정인지공능,지속6d,분별우수미궁실험제1、3、5、7천시수궤취10지대서처사,취뇌,분리해마,채용PCR화Western blot법검측해마철조소화FP1적표체.결과 여C조비교,ST조수미궁실험제3、4、5천시대서도피잠복기연장,원평태상한정류시간축단,천월원평태차수감소(P＜0.05),각시점해마철조소표체상조,FP1표체하조(P＜0.05).결론 수술창상가강저노령대서인지공능,기궤제가능여상조해마철조소표체,하조FP1표체유관.
Objective To evaluate the effect of surgical trauma on the cognitive function and expression of hepcidin and ferroportin 1 (FP1) in hippocampus in aged rats.Methods One hundred male Sprague-Dawley rats,aged 18 months,weighing 400-500 g,were randomly divided into 2 groups with 50 rats in each group:control group (group C) and surgical trauma group (group ST).The rats were anesthetized with chloral hydrate,but underwent no operation in group C.The rats Were anesthetized with chloral hydrate and underwent 30 min of modified exploratory laparotomy in group ST.Ten rats were chosen from each group at 24 h after operation and the cognitive function was assessed using Morris water-maze test for 6 consecutive days.Ten rats were sacrificed on 1st,3rd,5th and 7th days after beginning of Morris water-maze test and brains were removed for determination of hepcidin and FP1 expression in hippocampus by PCR and Western blot.Results Compared with group C,the escape latency was significantly prolonged,the time of staying at the original platform quadrant and frequency of crossing the original platform were decreased on 3rd,4th and 5th days after beginning of Morris water-maze test,and the expression of hepcidin was up-regulated and the expression of FP1 was down-regulated at each time point in group ST (P ＜ 0.05).Conclusion Surgical trauma can decrease the cognitive function in aged rats and the mechanism may be related to up-regulation of hepcidin expression and down-regulation of FP1 expression in hippocampus.