CAJ | 학술논문

目的评价2型糖尿病因素对舒芬太尼后处理减轻大鼠心肌缺血再灌注损伤的影响.方法健康雄性清洁级SD大鼠,体重160～ 180 g.采用高脂饲料喂养诱导胰岛素抵抗+腹腔注射链脲佐菌素方法制备2型糖尿病模型.取造模成功的大鼠18只,采用随机数字表法,将其分为3组(n=6):糖尿病假手术组(DM-S组)、糖尿病缺血再灌注组(DM-I/R组)、糖尿病舒芬太尼后处理组(DM-SP组).另取健康大鼠18只,采用随机数字表法,将其分为3组(n=6):非糖尿病假手术组(NDM-S组)、非糖尿病缺血再灌注组(NDM-I/R组)、非糖尿病舒芬太尼后处理组(NDM-SP组).采用左冠状动脉前降支下穿线,平衡30 min,阻断30 min,再灌注120 min的方法制备心肌缺血再灌注损伤模型.NDM-SP组和DM-SP组于再灌注前5 min经右颈静脉注射舒芬太尼1.0 μg/kg.于缺血前即刻、缺血30 min、再灌注120 min(T1-3)时记录MAP、SP和HR,计算SP和HR乘积(RPP).于再灌注120 min时经右颈动脉采集血样,测定血浆cTnⅠ浓度.处死后取心脏,测定心肌梗死体积.结果非糖尿病与糖尿病大鼠心肌缺血再灌注时MAP、RPP降低,血浆cTnⅠ浓度升高,心肌发生梗死样改变.舒芬太尼后处理可减小非糖尿病大鼠心肌缺血再灌注时心肌梗死体积,降低血浆cTnI浓度,升高MAP和RPP,但对糖尿病大鼠心肌缺血再灌注时各指标无明显影响.结论 2型糖尿病因素可阻碍舒芬太尼后处理对大鼠的心肌保护作用.
목적 평개2형당뇨병인소대서분태니후처리감경대서심기결혈재관주손상적영향.방법 건강웅성청길급SD대서,체중160～ 180 g.채용고지사료위양유도이도소저항+복강주사련뇨좌균소방법제비2형당뇨병모형.취조모성공적대서18지,채용수궤수자표법,장기분위3조(n=6):당뇨병가수술조(DM-S조)、당뇨병결혈재관주조(DM-I/R조)、당뇨병서분태니후처리조(DM-SP조).령취건강대서18지,채용수궤수자표법,장기분위3조(n=6):비당뇨병가수술조(NDM-S조)、비당뇨병결혈재관주조(NDM-I/R조)、비당뇨병서분태니후처리조(NDM-SP조).채용좌관상동맥전강지하천선,평형30 min,조단30 min,재관주120 min적방법제비심기결혈재관주손상모형.NDM-SP조화DM-SP조우재관주전5 min경우경정맥주사서분태니1.0 μg/kg.우결혈전즉각、결혈30 min、재관주120 min(T1-3)시기록MAP、SP화HR,계산SP화HR승적(RPP).우재관주120 min시경우경동맥채집혈양,측정혈장cTnⅠ농도.처사후취심장,측정심기경사체적.결과 비당뇨병여당뇨병대서심기결혈재관주시MAP、RPP강저,혈장cTnⅠ농도승고,심기발생경사양개변.서분태니후처리가감소비당뇨병대서심기결혈재관주시심기경사체적,강저혈장cTnI농도,승고MAP화RPP,단대당뇨병대서심기결혈재관주시각지표무명현영향.결론 2형당뇨병인소가조애서분태니후처리대대서적심기보호작용.
Objective To investigate the effect of type 2 diabetes mellitus (DM) on the attenuation of myocardial ischemia-reperfusion (I/R) injury by sufentanil postconditioning in rats.Methods Male pathogen-free Sprague-Dawley rats,weighing 160-180 g,were used in the study.A model for type 2 DM was established by the feeding of high-fat diet-induced insulin resistance and intraperitoneal streptozocin 35 mg/kg.DM was confirmed by blood glucose level ≥ 16.7 mmol/L one week after injection.Eighteen type 2 diabetic rats were randomly divided into 3 groups (n =6 each):DM sham operation group (DM-S group) ; DM-I/R group; DM sufentanil postconditioning group (DM-SP group).Another 18 healthy nondiabetic rats were chosen and randomly divided into 3 groups (n =6 each):nondiabetes mellitus sham operation group (NDM-S group) ; nondiabetes mellitus I/R group (NDM-I/R group) ; nondiabetes mellitus sufentanil postconditioning group (NDM-SP group).Myocardial I/R was induced by 30 min occlusion of the left anterior descending branch of coronary artery (after 30 min of equilibration) followed by 120 min of reperfusion.Sufentanil 1.0 μg/kg was injected via the right jugular vein 5 min before reperfusion in NDM-SP and DM-SP groups.MAP,SP and HR were recorded immediately before ischemia,at 30 min of ischemia and at 120 min of reperfusion and rate-pressure product (RPP) was calculated.Arterial blood samples were collected at 120 min of reperfusion for measurement of plasma cardiac troponin Ⅰ (cTnⅠ) concentration.The rats were then sacrificed for determination of the myocardial infract size.Results MAP and RPP were decreased,while the plasma cTnl concentration was increased during reperfusion in diabetic and nondiabetic rats.Sufentanil postconditioning decreased the myocardial infract size and plasma concentrations of cTnⅠ,and increased MAP and RPP during reperfusion in nondiabetic rats,but had no effect on the parameters in diabetic rats.Conclusion Type 2 DM interferes with sufentanil postconditioning-induced myocardial protection in rats.