CAJ | 학술논문

目的评价富氢液对大鼠全脑缺血再灌注时海马微小RNA 210(miR-210)和miR-21表达的影响.方法健康雄性SD大鼠72只,9～ 10周龄,体重250～ 300 g,采用随机数字表法,将其分为3组(n=24):假手术组(S组)、缺血再灌注组(I/R组)和富氢液组(H组).采用四血管阻塞法制备大鼠全脑缺血再灌注损伤模型.H组于再灌注即刻、6h时腹腔注射0.6 mmol/L富氢液5 ml/kg,S组和I/R组以等容量生理盐水替代.于再灌注24、72 h时处死,取双侧海马组织,采用实时定量逆转录PCR法检测miR-210和miR-21的表达水平,另取全脑组织,行HE染色,光镜下观察海马CA1区病理学结果,并进行锥体细胞计数.结果与S组比较,I/R组miR-210和miR-21表达上调,锥体细胞计数减少(P＜0.05)；与I/R组比较,H组miR-210和miR-21表达下调,锥体细胞计数增多(P＜0.05).S组海马CA1区锥体细胞正常,I/R组锥体细胞病理学损伤明显,H组锥体细胞病理学损伤程度减轻.结论富氢液减轻大鼠全脑缺血再灌注损伤的机制与下调海马miR-210和miR-21表达有关.
목적 평개부경액대대서전뇌결혈재관주시해마미소RNA 210(miR-210)화miR-21표체적영향.방법 건강웅성SD대서72지,9～ 10주령,체중250～ 300 g,채용수궤수자표법,장기분위3조(n=24):가수술조(S조)、결혈재관주조(I/R조)화부경액조(H조).채용사혈관조새법제비대서전뇌결혈재관주손상모형.H조우재관주즉각、6h시복강주사0.6 mmol/L부경액5 ml/kg,S조화I/R조이등용량생리염수체대.우재관주24、72 h시처사,취쌍측해마조직,채용실시정량역전록PCR법검측miR-210화miR-21적표체수평,령취전뇌조직,행HE염색,광경하관찰해마CA1구병이학결과,병진행추체세포계수.결과 여S조비교,I/R조miR-210화miR-21표체상조,추체세포계수감소(P＜0.05)；여I/R조비교,H조miR-210화miR-21표체하조,추체세포계수증다(P＜0.05).S조해마CA1구추체세포정상,I/R조추체세포병이학손상명현,H조추체세포병이학손상정도감경.결론 부경액감경대서전뇌결혈재관주손상적궤제여하조해마miR-210화miR-21표체유관.
Objective To evaluate the effects of hydrogen-rich saline on the expression of miR-210 and miR-21 in hippocampus during global cerebral ischemia-reperfusion (I/R) in rats.Methods Seventy-two healthy male Sprague-Dawley rats,aged 9-10 weeks,weighing 250-300 g,were randomly divided into 3 groups (n =24 each):sham operation group (group S),group I/R,and hydrogen-rich saline group (group H).Global cerebral I/R was produced by 4-vessel occlusion method.In group H,0.6 mmol/L hydrogen-rich saline 5 ml/kg was injected intraperitoneally at 0 and 6 h of reperfusion,while the equal volume of normal saline was injected instead of hydrogen-rich saline in the other two groups.Rats were sacrificed at 24 and 72 h of reperfusion,and then the bilateral hippocampi were removed for detection of the expression of miR-210 and miR-21 using RT-PCR.The global brain tissues were also obtained and stained with HE for examination of the changes in pyramidal cells in the CA1 region of hippocampus.Results Compared with group S,the expression of miR-210 and miR-21 was significantly up-regulated,and the number of pyramidal cells was decreased in group I/R (P ＜ 0.05).Compared with group I/R,the expression of miR-210 and miR-21 was significantly down-regulated,and the number of pyramidal cells was increased in group H (P ＜ 0.05).The pathological changes were significantly ameliorated in group H.Conciusion The mechanism by which hydrogen-rich saline attenuates global cerebral I/R injury is related to downregulation of the expression of miR-210 and miR-21 in rat hippocampus.