CAJ | 학술논문

目的评价腹腔注射不同剂量异丙酚对哮喘小鼠气道高反应性、肺部炎症以及Th1/Th2平衡的调节作用.方法健康雌性BALB/c小鼠100只,6～8周龄,体重18 ～ 20 g,采用随机数字表法,将小鼠分为5组(n=20)∶对照组(C组)、哮喘组(A组)、低剂量异丙酚组(50 mg/kg i.p.,LP组)、中等剂量异丙酚组(100 mg/kg i.p.,MP组)和高剂量异丙酚组(150 mg/kg i.p.,HP组).使用卵清蛋白(OVA)构建过敏性哮喘模型小鼠,腹腔注射-诱导过敏性哮喘模型.各组随机取10只于模型构建成功后24 h即高剂量麻醉处死,行心脏取血及支气管肺泡灌洗,以ELISA法检测血清OVA特异性IgE和支气管肺泡灌洗液中特征性细胞因子IL-4、IL-5、IFN-γ浓度,并行细胞计数/分类计数.各组剩余的10只于模型构建成功后24h行肺功能检测和取肺进行HE染色,比较各组小鼠气道反应性和肺组织的病理组织学评分.结果腹腔注射异丙酚(LP组和MP组)可明显地抑制哮喘小鼠的气道高反应性.不同剂量异丙酚干预组(LP组、MP组和HP组)均可明显抑制哮喘小鼠肺组织中嗜酸性粒细胞浸润,降低IL-4、IL-5和血清OVA特异性IgE水平,上调IFN-γ/IL-4比值.结论异丙酚能够有效抑制哮喘小鼠肺部炎症,上调Th1/Th2比值,改善肺功能,发挥气道保护作用.
목적 평개복강주사불동제량이병분대효천소서기도고반응성、폐부염증이급Th1/Th2평형적조절작용.방법 건강자성BALB/c소서100지,6～8주령,체중18 ～ 20 g,채용수궤수자표법,장소서분위5조(n=20)∶대조조(C조)、효천조(A조)、저제량이병분조(50 mg/kg i.p.,LP조)、중등제량이병분조(100 mg/kg i.p.,MP조)화고제량이병분조(150 mg/kg i.p.,HP조).사용란청단백(OVA)구건과민성효천모형소서,복강주사-유도과민성효천모형.각조수궤취10지우모형구건성공후24 h즉고제량마취처사,행심장취혈급지기관폐포관세,이ELISA법검측혈청OVA특이성IgE화지기관폐포관세액중특정성세포인자IL-4、IL-5、IFN-γ농도,병행세포계수/분류계수.각조잉여적10지우모형구건성공후24h행폐공능검측화취폐진행HE염색,비교각조소서기도반응성화폐조직적병리조직학평분.결과 복강주사이병분(LP조화MP조)가명현지억제효천소서적기도고반응성.불동제량이병분간예조(LP조、MP조화HP조)균가명현억제효천소서폐조직중기산성립세포침윤,강저IL-4、IL-5화혈청OVA특이성IgE수평,상조IFN-γ/IL-4비치.결론 이병분능구유효억제효천소서폐부염증,상조Th1/Th2비치,개선폐공능,발휘기도보호작용.
Objective To investigate the immunoregulatory effects of propofol on airway hyperresponsiveness,airway inflammation and Thl/Th2 ratio in the asthmatic mice.Methods One hundred female BALB/c mice,aged 6-8 weeks,weighing 18-20 g,were randomly divided into 5 groups (n =20,each):control group (normal saline i.p.,group C),asthma group (group A),low-dose propofol (50 mg/kg i.p.,group LP),medium-dose propofol (100 mg/kg i.p.,group MP) and high-dose propofol (150 mg/kg i.p.,group HP).Mice of groups A,LP,MP and HP were sensitized with ovalbumin (OVA),mice of group C were sensitized with normal saline.24 h after the last challenge,animals were sacrificed by lethal dose of pentobarbital sodium.Blood and bronchoalveolar lavage fluid (BALF) were collected for determination of serum OVA-specific IgE and the levels of cytokines (IL-4,IL-5 and IFN-γ) in the BALF.Airway responsiveness was measured by the forced-oscillation technique and histological inflammation scores were measured by staining with hematoxylin and eosin.Results Propofol (group LP and group MP) attenuated airway hyperresposiveness to the muscarinic agonist methacholine in OVA-induced asthma.Different doses of propofol (group LP,group MP and group HP) decreased eosinoplils influx in lungs.In addition,propofol treatment reduced expression of IL-4,IL-5 and serum OVA-specific IgE and increased the ratio of IFN-γ/IL-4.Conclusion The study demonstrates a potential protective value of propofol in alleviating airway inflammation,up-regulating Th1/Th2 ratio and attenuating airway hyperresposiveness in the asthmatic mice.