CAJ | 학술논문

目的评价乳化异氟醚预处理对大鼠肾缺血再灌注损伤的影响.方法选择雄性SD大鼠32只,体重220～300 g,10～ 13周龄,采用随机数字表法,将其分为4组(n=8):假手术组(S组)、肾缺血再灌注组(I/R组)、乳化异氟醚预处理组(E组)和脂肪乳剂预处理组(I组).I/R组、E组和I组采用夹闭左侧肾蒂45 min后恢复再灌注的方法建立肾缺血再灌注模型.E组和I组分别静脉输注8%乳化异氟醚、30%脂肪乳剂4ml·kg-·h-30 min,洗脱15 min后制备模型.于再灌注3h时采集腹主动脉血样5ml,测定血清肌酐(Cr)、胱抑素C(Cys C)、TNF-α、IL-6、IL-10浓度；取左肾组织,行HE染色,光镜下观察病理学结果,并行肾脏近曲小管坏死程度分级.结果与S组比较,其余3组血清Cr、Cys C、TNF-α、IL-6、IL-10浓度及肾脏近曲小管坏死程度均升高(P＜0.05)；与I/R组和I组比较,E组血清Cr、Cys C、TNF-α、IL-6浓度及肾脏近曲小管坏死程度均降低(P＜0.05),血清IL-10浓度升高(P＜0.05).I/R组和I组上述各指标比较差异无统计学意义(P＞0.05).I/R组和I组肾组织病理学损伤严重,E组肾组织病理学损伤明显减轻.结论 8％乳化异氟醚预处理可减轻大鼠肾缺血再灌注损伤,其机制可能与抑制全身炎性反应有关.
목적 평개유화이불미예처리대대서신결혈재관주손상적영향.방법 선택웅성SD대서32지,체중220～300 g,10～ 13주령,채용수궤수자표법,장기분위4조(n=8):가수술조(S조)、신결혈재관주조(I/R조)、유화이불미예처리조(E조)화지방유제예처리조(I조).I/R조、E조화I조채용협폐좌측신체45 min후회복재관주적방법건립신결혈재관주모형.E조화I조분별정맥수주8%유화이불미、30%지방유제4ml·kg-·h-30 min,세탈15 min후제비모형.우재관주3h시채집복주동맥혈양5ml,측정혈청기항(Cr)、광억소C(Cys C)、TNF-α、IL-6、IL-10농도；취좌신조직,행HE염색,광경하관찰병이학결과,병행신장근곡소관배사정도분급.결과 여S조비교,기여3조혈청Cr、Cys C、TNF-α、IL-6、IL-10농도급신장근곡소관배사정도균승고(P＜0.05)；여I/R조화I조비교,E조혈청Cr、Cys C、TNF-α、IL-6농도급신장근곡소관배사정도균강저(P＜0.05),혈청IL-10농도승고(P＜0.05).I/R조화I조상술각지표비교차이무통계학의의(P＞0.05).I/R조화I조신조직병이학손상엄중,E조신조직병이학손상명현감경.결론 8％유화이불미예처리가감경대서신결혈재관주손상,기궤제가능여억제전신염성반응유관.
Objective To evaluate the effect of 8% emulsified isoflurane preconditioning on renal ischemia-reperfusion (I/R) injury in rats.Methods Thirty-two male Sprague-Dawley rats,aged 10-13 weeks,weighing 220-300 g,were randomly divided into 4 groups (n =8 each):sham operation group (group S); I/R group;emulsified isoflurane preconditioning group (group E) ; intralipid preconditioning group (group I).Renal ischemia was induced by occlusion of the left renal pedicle for 45 min with atraumatic microclips followed by 3 h reperfusion.8 % emulsified isoflurane and 30 % intralipid 4 ml· kg-1· h-1 were infused intravenously for 30 min followed by 15 min washout before renal UR in groups E and I,respectively.Arterial blood samples were taken at 3 h of reperfusion to determine the concentrations of serum creatinine (Cr),cystatin C (Cys C),TNF-α,IL-6 and IL-10.The animals were then sacrificed and left kidneys were removed and stained with hematoxylin-eosin for microscopic examination and assessment of necrosis of renal proximal convoluted tubules (0 =normal,4 =necrosis of whole segment of proximal convoluted tubules).Results Compared with group S,the serum Cr,Cys C,TNF-α,IL-6 and IL-10 concentrations and severity of necrosis of renal proximal convoluted tubules were significantly increased in groups I/R,E and I (P ＜ 0.05).The serum Cr,Cys C,TNF-α and IL-6 concentrations and severity of necrosis of renal proximal convoluted tubules were significantly lower,while the serum IL-10 concentration was higher in group E than in groups I/R and I (P ＜0.05).There was no significant difference in the indexes mentioned above between groups L and I/R (P ＞ 0.05).The damage to renal tissues was less serious in group E than in groups I/R and L.Conclusion Preconditioning with 8 % emulsified isoflurane can attenuate renal I/R injury by inhibiting inflammatory responses in rats.