CAJ | 학술논문

目的探讨糖原合成酶激酶-3β(GSK-3β)在瑞芬太尼诱发大鼠脊髓背角神经元NMDA受体微小兴奋性突触后膜电流(mEPSCs)中的作用.方法取出生14～ 18 d的Wistar大鼠24只,体重50 ～ 60 g,制备腰段脊髓切片,取切片144张,采用随机数字表法,将其分为4组(n=36):对照组(C组):置入人工脑脊液中培养；甘氨酸组(G组):置入含甘氨酸(浓度0.24 μmol/L)的人工脑脊液中孵育；瑞芬太尼组(R组):置入含瑞芬太尼(浓度为4 nmol/L)的人工脑脊液中孵育；瑞芬太尼+GSK-3β抑制剂TDZD-8组(RT组):置入含瑞芬太尼和TDZD-8(浓度分别为4 nmol/L和10 μmol/L)的人工脑脊液中孵育.各组孵育60 min后应用全细胞膜片钳技术记录脊髓背角神经元NMDA受体mEPSCs的幅值和频率.结果与C组比较,R组mEPSCs的幅值和频率升高(P＜0.01),G组和RT组差异无统计学意义(P＞ 0.05)；与R组比较,RT组mEPSCs幅值和频率降低(P＜0.01).结论脊髓背角神经元GSK-3β参与了瑞芬太尼增强NMDA受体mEPSCs的作用,提示其可能参与了瑞芬太尼诱发的痛觉过敏.
목적 탐토당원합성매격매-3β(GSK-3β)재서분태니유발대서척수배각신경원NMDA수체미소흥강성돌촉후막전류(mEPSCs)중적작용.방법 취출생14～ 18 d적Wistar대서24지,체중50 ～ 60 g,제비요단척수절편,취절편144장,채용수궤수자표법,장기분위4조(n=36):대조조(C조):치입인공뇌척액중배양；감안산조(G조):치입함감안산(농도0.24 μmol/L)적인공뇌척액중부육；서분태니조(R조):치입함서분태니(농도위4 nmol/L)적인공뇌척액중부육；서분태니+GSK-3β억제제TDZD-8조(RT조):치입함서분태니화TDZD-8(농도분별위4 nmol/L화10 μmol/L)적인공뇌척액중부육.각조부육60 min후응용전세포막편겸기술기록척수배각신경원NMDA수체mEPSCs적폭치화빈솔.결과 여C조비교,R조mEPSCs적폭치화빈솔승고(P＜0.01),G조화RT조차이무통계학의의(P＞ 0.05)；여R조비교,RT조mEPSCs폭치화빈솔강저(P＜0.01).결론 척수배각신경원GSK-3β삼여료서분태니증강NMDA수체mEPSCs적작용,제시기가능삼여료서분태니유발적통각과민.
Objective To investigate the role of glycogen synthesis kinase-3β (GSK-3β3) in remifentanilinduced N-methyl-D-aspartate (NMDA) receptor-mediated miniature excitatory postsynaptic currents (mEPSCs) in rat spinal dorsal horn neurons.Methods Twenty-four Wistar rats,aged 14-18 days,weighing 50-60 g,were used.L1-S11 segments of the spinal cord were removed for preparation of spinal cord slices.The slices were randomly divided into 4 groups (n =36 each):control group (group C),glycine group (group G),remifentanil group (group R),and remifentanil + GSK-3β inhibitor TDZD-8 group (group RT).The animals were anesthetized with intraperitoneal chloral hydrate 400 mg/kg.The slices were incubated in plain artificial cerebrospinal fluid (ACSF)for 60 min in group C.The slices were incubated for 60 min in ACSF containing glycine 0.24 μmol/L,remifentanil 4 nmol/L,and remifentanil 4 nmol/L+ TDZD-8 10 μmol/L in groups G,R and RT,respectively.The whole-cell patch-clamp technique was used to record the amplitude and frequency of NMDA receptor mEPSCs in spinal dorsal horn neurons.Results Compared with group C,the amplitude and frequency of mEPSCs were significantly increased in group R (P ＜ 0.01),and no significant change was found in groups G and RT (P ＞ 0.05).Compared with group R,the amplitude and frequency of mEPSCs were significantly decreased in group RT (P ＜ 0.01).Conclusion GSK-3β in spinal dorsal horn neurons is involved in remifentanil-induced enhancement of NMDA receptor mEPSCs,indicating that it may be involved in remifentanil-induced hyperalgesia.